BIOL 1050 PRACTICAL EXAM
QUESTIONS WITH CORRECT
ANSWERS
What is the difference between a paired design experiment and an unpaired design
experiment? - Correct Answers -A paired experiment inovles the same subjects
w/different treatmnets. An unpaired design experiment involves different subjects w/the
same treatment.
What is H0? - Correct Answers -Null Hypothesis; opposite of what you expect
What is H1? - Correct Answers -Alternative Hypothesis; what you EXPECT to happen
What does a P value less than 0.05 indicate? - Correct Answers -The results are
statically significant; they prove the alternative hypothesis
What does a P value greater than 0.05 indicate? - Correct Answers -The results are not
significant; the null hypothesis is accepted
What color should the Bradford reagent appear in the presence of protein? - Correct
Answers -Blue
In the regression equation, what does the y and x values stand for?
Example: y = 0.0167x + 0.1496 - Correct Answers -y: absorbance
x: protein concentration
Use the standard cure plot to convert absorbance values to a concentration.
,Example: An absorbance of 0.16 was obtained from the spectrophotometer. Using the
graph below determine the protein concentration of this sample. - Correct Answers -100
mg/dL
Using regression equation, convert absorbance value to protein concentration.
An absorbance value of 0.4 was obtained for a protein sample. Using the regression
equation provided, what is the protein concentration in the sample? y = 0.174x - 0.024 -
Correct Answers -2.44
The following information is stamped on serological pipettes: "1 mL in 1/100". What is
the size of this pipette, in terms of volume capacity?s - Correct Answers -1 mL
Based on the stamp at the top of this serological pipette how many mL of liquid can it
hold? - Correct Answers -10 mL
Percent Solution Formula - Correct Answers -% desired = amount desired / 100mL of
fluid
Describe how you would prepare 280mL of a 1.8% solution of Na2CO3. - Correct
Answers -Percent Solution
(1.8) * (280/100) = 5.04 g Na2CO3 in 280mL of water
Percent by Volume Solution Equation - Correct Answers -C1V1=C2V2
, Describe how you would prepare 210mL of 0.6% Na2Co3 solution from a 1.8% stock
solution using the percent by volume method. - Correct Answers -Percent by Volume
(60)(210) = (180)(x) = 70 mL of stock
How many mL of a 2.9% Na2CO3 stock solution would you add to make 300 mL of a
0.7% Na2CO3 solution? (C1V1=C2V2) - Correct Answers -72.41 mL
Molar Solution Equation - Correct Answers -Grams required = molecular weight *
volume desired in liters * molarity desired
250 mL to liters - Correct Answers -Divide by 1000; 0.25
How would you prepare 250mL of a 0.1 M Na2Co3 solution. - Correct Answers -106g *
0.25 *0.1 = 2.65 g Na2Co3
What is the equation used to determine pOH from pH measured for a solution?
Example: If a given solution has a pH of 6 what is the pOH of that solution? - Correct
Answers -pH + pOH = 14
6 + x = 14
pOH = 8
Know how to determine H+, OH- from pH measured for a solution. - Correct Answers -
pH = -log[H+]
pOH = -log[OH-]
[H+] = 10^-pH
[OH-] = 10^-pOH
If given a pH of 11 calculate the [H+] concentration of the solution. - Correct Answers -
pH = -log[H+]
1x10^-11
If given a hydroxide ion concentration [OH-] of 6.16595 × 10-5 what is the pOH of the
solution? - Correct Answers -pOH = -log[OH-]
4.21
pH = 6, [H+] = ? - Correct Answers -10^-6 = 1 x 10^-6
pOH = 13, [OH-] = ? - Correct Answers -10^-13 = 1 x 10^-13
QUESTIONS WITH CORRECT
ANSWERS
What is the difference between a paired design experiment and an unpaired design
experiment? - Correct Answers -A paired experiment inovles the same subjects
w/different treatmnets. An unpaired design experiment involves different subjects w/the
same treatment.
What is H0? - Correct Answers -Null Hypothesis; opposite of what you expect
What is H1? - Correct Answers -Alternative Hypothesis; what you EXPECT to happen
What does a P value less than 0.05 indicate? - Correct Answers -The results are
statically significant; they prove the alternative hypothesis
What does a P value greater than 0.05 indicate? - Correct Answers -The results are not
significant; the null hypothesis is accepted
What color should the Bradford reagent appear in the presence of protein? - Correct
Answers -Blue
In the regression equation, what does the y and x values stand for?
Example: y = 0.0167x + 0.1496 - Correct Answers -y: absorbance
x: protein concentration
Use the standard cure plot to convert absorbance values to a concentration.
,Example: An absorbance of 0.16 was obtained from the spectrophotometer. Using the
graph below determine the protein concentration of this sample. - Correct Answers -100
mg/dL
Using regression equation, convert absorbance value to protein concentration.
An absorbance value of 0.4 was obtained for a protein sample. Using the regression
equation provided, what is the protein concentration in the sample? y = 0.174x - 0.024 -
Correct Answers -2.44
The following information is stamped on serological pipettes: "1 mL in 1/100". What is
the size of this pipette, in terms of volume capacity?s - Correct Answers -1 mL
Based on the stamp at the top of this serological pipette how many mL of liquid can it
hold? - Correct Answers -10 mL
Percent Solution Formula - Correct Answers -% desired = amount desired / 100mL of
fluid
Describe how you would prepare 280mL of a 1.8% solution of Na2CO3. - Correct
Answers -Percent Solution
(1.8) * (280/100) = 5.04 g Na2CO3 in 280mL of water
Percent by Volume Solution Equation - Correct Answers -C1V1=C2V2
, Describe how you would prepare 210mL of 0.6% Na2Co3 solution from a 1.8% stock
solution using the percent by volume method. - Correct Answers -Percent by Volume
(60)(210) = (180)(x) = 70 mL of stock
How many mL of a 2.9% Na2CO3 stock solution would you add to make 300 mL of a
0.7% Na2CO3 solution? (C1V1=C2V2) - Correct Answers -72.41 mL
Molar Solution Equation - Correct Answers -Grams required = molecular weight *
volume desired in liters * molarity desired
250 mL to liters - Correct Answers -Divide by 1000; 0.25
How would you prepare 250mL of a 0.1 M Na2Co3 solution. - Correct Answers -106g *
0.25 *0.1 = 2.65 g Na2Co3
What is the equation used to determine pOH from pH measured for a solution?
Example: If a given solution has a pH of 6 what is the pOH of that solution? - Correct
Answers -pH + pOH = 14
6 + x = 14
pOH = 8
Know how to determine H+, OH- from pH measured for a solution. - Correct Answers -
pH = -log[H+]
pOH = -log[OH-]
[H+] = 10^-pH
[OH-] = 10^-pOH
If given a pH of 11 calculate the [H+] concentration of the solution. - Correct Answers -
pH = -log[H+]
1x10^-11
If given a hydroxide ion concentration [OH-] of 6.16595 × 10-5 what is the pOH of the
solution? - Correct Answers -pOH = -log[OH-]
4.21
pH = 6, [H+] = ? - Correct Answers -10^-6 = 1 x 10^-6
pOH = 13, [OH-] = ? - Correct Answers -10^-13 = 1 x 10^-13
