GMU BIO 306 Lab Quiz -3 2025

6
Lab Quiz 3
Course 306 Total Point-15
1r
Name:

lQ. Examine the diagram below of a culture tube. Based on the growth pattern observed,
--
identify the oxygen requirement of the bacteria cultured in tube B. Pt 1
A B C D E



oxygen requirement: ob\; ~ ~ q lit~~ t
(~h ds 4t '"'1 \,,H4
,~)



ot•O\},c_


v
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Pt 1x2=2
2Q. The instructor asks the student to perform 1:10 dilution using the provided
volume illustrated on Figure 1. The instructor hands the student an aliquot of the P200
stock culture that has a measured CFU/ml count of 125x105. {vo' -r o;II.JHr»)

m
0




i) Please calculate the required diluent (in ml) needed to successfully
perform~this dilution.
ii) What will be the CFU/ml after dilution? 2
I '2. 5
5
\JO\. of soi "i\t ~ "o\. of
Q',\\,i,,<'




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3Q. If 0.1 ml of a 10- dilution of a culture is plated out and 60 colonies appear after incubation,
6
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what will be the CFU/ml in undiluted culture? Pt 2

'vo
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4Q. For collecting a bacterial sample, you used P20 pipetman which is set as follows. How much
volume of the sample can you take with this set up? Pt 1
0

: Volume:-~_•~ - µ I /
(P20 pipetman) .L