Lab Quiz-2
Total Points 15
Name:
lQ. Define:
Course 306
Pt 1x2=2
--
one
r,J ,,,,,, uo oT:;;P' a le ne·
Pure culture: A" c vi 1\J v(_
cl ,f l'.r~,o-k I, rfy d1{~,€n-J
Differential stain: Jr pe of helps fe(
ct.-a,qcl-1,,,·;t,c.5
Jey,~
{ex ::-711 rJ )
y_, s rvql J ,~ r,,
bcn-f,,,, o
2Q. You are given a bacterial culture. After Gram staining, you have found that it is Gram
negative rod shaped. What selective and differential media will you use in the next step to
identify if these unknown bacteria are Escherichia coli? Pt 1
Et16 ( £OS·, A 5\ve.) Aj C\ y ,,,,-
1It" t
-
\
/\'\ r1'n
b·S
~
• ,M a h V\ ·, ~ \ S '1 I+
Ay,-•~?
~
/\'.
• .J
3Q. Fill in the Blanks: Pt lxS= 5
______l....,.p-'h_o.__k_e_1")_01....t.S ~ 5 /
a) Partial breakdown of red blood cells: _A
b) No breakdown of red blood cells: G&I"" rn 'I hPrr, •~Si J /
-
_C_,; . o.&. ; ;,lo,_;_r_ _ in the media. 'f
---
c) EMB (Eosin-Methylene Blue) agar is a differential media because of the presence of
d) Upon treatment with 95% ethanol, the cell wall of j '•"' f'Sil:ve bacte~ comes
dehydrated, trapping the dye inside the cell.
e) If the bacterium growfng on MSA (Mannitol Salt Agar) plate, the bacterium produce ~ ci ~ v /
~
•
\J which change the PH and the agar turns from red to yellow.
•
.,.
4Q. After decolorization step, what will be the color of Gram-negative bacteria? Pt 1
A p I() I( / red Color
J
Total Points 15
Name:
lQ. Define:
Course 306
Pt 1x2=2
--
one
r,J ,,,,,, uo oT:;;P' a le ne·
Pure culture: A" c vi 1\J v(_
cl ,f l'.r~,o-k I, rfy d1{~,€n-J
Differential stain: Jr pe of helps fe(
ct.-a,qcl-1,,,·;t,c.5
Jey,~
{ex ::-711 rJ )
y_, s rvql J ,~ r,,
bcn-f,,,, o
2Q. You are given a bacterial culture. After Gram staining, you have found that it is Gram
negative rod shaped. What selective and differential media will you use in the next step to
identify if these unknown bacteria are Escherichia coli? Pt 1
Et16 ( £OS·, A 5\ve.) Aj C\ y ,,,,-
1It" t
-
\
/\'\ r1'n
b·S
~
• ,M a h V\ ·, ~ \ S '1 I+
Ay,-•~?
~
/\'.
• .J
3Q. Fill in the Blanks: Pt lxS= 5
______l....,.p-'h_o.__k_e_1")_01....t.S ~ 5 /
a) Partial breakdown of red blood cells: _A
b) No breakdown of red blood cells: G&I"" rn 'I hPrr, •~Si J /
-
_C_,; . o.&. ; ;,lo,_;_r_ _ in the media. 'f
---
c) EMB (Eosin-Methylene Blue) agar is a differential media because of the presence of
d) Upon treatment with 95% ethanol, the cell wall of j '•"' f'Sil:ve bacte~ comes
dehydrated, trapping the dye inside the cell.
e) If the bacterium growfng on MSA (Mannitol Salt Agar) plate, the bacterium produce ~ ci ~ v /
~
•
\J which change the PH and the agar turns from red to yellow.
•
.,.
4Q. After decolorization step, what will be the color of Gram-negative bacteria? Pt 1
A p I() I( / red Color
J
