1|Page
ATI Anatomy and Physiology Practice Exam (NEW UPDATED VERSION)
LATEST ACTUAL EXAM QUESTIONS AND CORRECT ANSWERS (VERIFIED
QUESTIONS AND ANSWERS)- GUARANTEED PASS A+ UPDATED 2026-2027
ATI Anatomy & Physiology Practice Exam
Cellular & Tissue Level
1. Which organelle is responsible for protein synthesis?
A. Mitochondria
B. Ribosome
C. Lysosome
D. Golgi apparatus
Answer: B. Ribosome
Rationale: Ribosomes synthesize proteins using mRNA instructions. Mitochondria produce
ATP, lysosomes digest cellular waste, and Golgi modifies and packages proteins.
2. Which of the following are functions of the plasma membrane? (Select all that apply)
A. Regulate transport of substances
B. Generate ATP
C. Provide structural support
D. Store genetic information
Answer: A, C
Rationale: The plasma membrane controls substance entry/exit and provides structural support.
ATP is made in mitochondria, and genetic information is stored in the nucleus.
3. During mitosis, which phase involves separation of sister chromatids?
A. Prophase
B. Metaphase
C. Anaphase
D. Telophase
Answer: C. Anaphase
Rationale: Anaphase is when sister chromatids are pulled apart to opposite poles. Prophase
condenses chromosomes, metaphase aligns them, telophase reforms nuclei.
2026 2027 GRADED A+
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4. Which tissue type lines body surfaces and cavities?
A. Epithelial
B. Connective
C. Muscle
D. Nervous
Answer: A. Epithelial
Rationale: Epithelial tissue forms linings and barriers. Connective supports, muscle contracts,
and nervous transmits impulses.
5. Which connective tissue stores fat?
A. Cartilage
B. Bone
C. Adipose
D. Dense regular
Answer: C. Adipose
Rationale: Adipose tissue stores fat, providing energy, insulation, and cushioning.
6. Which cell organelle contains digestive enzymes to break down cellular debris?
A. Ribosome
B. Lysosome
C. Endoplasmic reticulum
D. Golgi apparatus
Answer: B. Lysosome
Rationale: Lysosomes digest unwanted cellular material and pathogens.
7. Which type of epithelial tissue allows rapid diffusion of gases?
A. Stratified squamous
B. Simple squamous
C. Simple cuboidal
D. Pseudostratified columnar
Answer: B. Simple squamous
Rationale: Thin, single-layered simple squamous epithelium facilitates gas exchange in lungs.
2026 2027 GRADED A+
ATI Anatomy and Physiology Practice Exam (NEW UPDATED VERSION)
LATEST ACTUAL EXAM QUESTIONS AND CORRECT ANSWERS (VERIFIED
QUESTIONS AND ANSWERS)- GUARANTEED PASS A+ UPDATED 2026-2027
ATI Anatomy & Physiology Practice Exam
Cellular & Tissue Level
1. Which organelle is responsible for protein synthesis?
A. Mitochondria
B. Ribosome
C. Lysosome
D. Golgi apparatus
Answer: B. Ribosome
Rationale: Ribosomes synthesize proteins using mRNA instructions. Mitochondria produce
ATP, lysosomes digest cellular waste, and Golgi modifies and packages proteins.
2. Which of the following are functions of the plasma membrane? (Select all that apply)
A. Regulate transport of substances
B. Generate ATP
C. Provide structural support
D. Store genetic information
Answer: A, C
Rationale: The plasma membrane controls substance entry/exit and provides structural support.
ATP is made in mitochondria, and genetic information is stored in the nucleus.
3. During mitosis, which phase involves separation of sister chromatids?
A. Prophase
B. Metaphase
C. Anaphase
D. Telophase
Answer: C. Anaphase
Rationale: Anaphase is when sister chromatids are pulled apart to opposite poles. Prophase
condenses chromosomes, metaphase aligns them, telophase reforms nuclei.
2026 2027 GRADED A+
, 2|Page
4. Which tissue type lines body surfaces and cavities?
A. Epithelial
B. Connective
C. Muscle
D. Nervous
Answer: A. Epithelial
Rationale: Epithelial tissue forms linings and barriers. Connective supports, muscle contracts,
and nervous transmits impulses.
5. Which connective tissue stores fat?
A. Cartilage
B. Bone
C. Adipose
D. Dense regular
Answer: C. Adipose
Rationale: Adipose tissue stores fat, providing energy, insulation, and cushioning.
6. Which cell organelle contains digestive enzymes to break down cellular debris?
A. Ribosome
B. Lysosome
C. Endoplasmic reticulum
D. Golgi apparatus
Answer: B. Lysosome
Rationale: Lysosomes digest unwanted cellular material and pathogens.
7. Which type of epithelial tissue allows rapid diffusion of gases?
A. Stratified squamous
B. Simple squamous
C. Simple cuboidal
D. Pseudostratified columnar
Answer: B. Simple squamous
Rationale: Thin, single-layered simple squamous epithelium facilitates gas exchange in lungs.
2026 2027 GRADED A+
