ASCP STUDY GUIDE (MLT) EXAM
QUESTIONS AND ANSWERS 100% PASS
CK Is Associated with Cardiac, Muscles, & Brain Tissue. Elevated CK can be seen in Strokes,
Vigorous Exercise, and Muscular Dystrophy - ANS Elevated Serum Creatine Kinase Is
Associated with What Conditions?
Incubate at Room Temperature or 4oC to Enhance Weak Expression - ANS What Should Be
Done if Both Forward and Reverse ABO Results are NEGATIVE?
1.) Waist Line Over: 35 in Women, and 40 in Men
2.) Triglyceride Level over 150 mg/dL
3.) Fasting Blood Glucose over 100 mg/dL
4.) LDL Under: 50 in Women, and 40 in Men
5.) Blood Pressure ≥ 130/85 - ANS Parameters to define the presence of metabolic syndrome.
Class I: HLA-A, HLA-B, HLA-C Class II: HLA-DR - ANS Major Histocompatibility Complex
Antigens:
Class I & Class II
Accumulation of abnormal
inorganic acids, associated with diabetic ketoacidosis
1 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
,Excessive loss of bicarbonate, as in severe diarrhea
Renal Failure - ANS Metabolic Acidosis Caused By:
pH: Decreased
pCO2: Decreased - ANS Metabolic Acidosis Blood Gas Results:
Erythroid Value = Sum of all Nucleated RBC Precursors
Myeloid Value = Sum of Precursor Neutrophils, Eosinophils, and Basophils
*Lymphocytes and Monocytes are NOT counted*
Interpreted as overall Bone Marrow Cellularity - ANS Myeloid to Erythroid Ratio- (M:E) Ratio:
Myoglolbin - ANS Earliest Biochemical Marker for Myocardial Infraction
Lectin from the seeds of Dolichos Biflorus - ANS Anti-A1 Reagent is Made from What?
Rickettsia Rickettsii - ANS Rocky Mountain Spotted Fever is Transmitted by a Tick Infected
with what?
Oligopeptide in the blood that cause vasoconstriction of arterioles, increases blood pressure
and releases aldosterone. - ANS Angiotensin 2
Calcium and Phosphate - ANS Parathyroid Hormone Regulates what in the Body
Non-Pathogenic - ANS Barr Bodies are Classified as what?
2 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
,Interferon Gamma - ANS Cytokine most important for Increased Macrophage Cell Activity?
28 Days, unless the unit expires sooner. - ANS Shelf Life for Irradiated Blood?
MCV= 80-100 fL.
MCH= 27-31 pg.
MCHC= 32-36% - ANS Normal Ranges:
MCV
MCH
MCHC
MCV= (Hematocrit % / RBC's) x 10
MCH= (Hemoglobin / RBC's) x 10
MCHC= (Hemoglobin / Hematocrit) x 100 - ANS Formulas for:
MCV
MCH
MCHC
Muscle Mass - ANS Creatine Excretion Correlates be with:
37o C for 1 hour - ANS If not tested right away Semen should be stored:
Light Spin, Followed by a Hard Spin - ANS Procedure for preparation of Platelet concentrate
from whole-blood:
3 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
, Transferrin Saturation, due to its sensitivity and specificity for iron overload - ANS Best way to
test for Hereditary Hemochromatosis:
Positive QC: Pseudomonas Aeruginosa
Negative QC: Escherichia Coli - ANS Best Stock Cultures to perform QC testing of oxidase
production?
Middle Aged Adults - ANS What age group is Hereditary Hemochromatosis usually detected?
It is caused by the engrafting of immunocompetent T lymphocytes into a severely
immunosuppressed recipient. They can be prevented by gamma irradiation of cellular blood
components. - ANS What is responsible for causing Graft-Versus-Host Reactions?
Forward Scatter: Size
Side Scatter: Granularity - ANS In Flow Cytometry, Forward Scatter and Side Scatter tell us
what about the cells?
Eosinophils. Crystals have a strong association with parasitic infections or allergic reactions -
ANS Charcot-Leyden Crystals in stool are breakdown products of what?
Used to calculate LDL Cholesterol.
LDL = [Total Cholesterol] - [HDL] - [Triglyceride/5] - ANS What is the Friedewald Formula, and
what does it calculate?
Megoblastic Anemia, and Lead Poisoning - ANS What Diseases are Cabot Rings associated
with?
4 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
QUESTIONS AND ANSWERS 100% PASS
CK Is Associated with Cardiac, Muscles, & Brain Tissue. Elevated CK can be seen in Strokes,
Vigorous Exercise, and Muscular Dystrophy - ANS Elevated Serum Creatine Kinase Is
Associated with What Conditions?
Incubate at Room Temperature or 4oC to Enhance Weak Expression - ANS What Should Be
Done if Both Forward and Reverse ABO Results are NEGATIVE?
1.) Waist Line Over: 35 in Women, and 40 in Men
2.) Triglyceride Level over 150 mg/dL
3.) Fasting Blood Glucose over 100 mg/dL
4.) LDL Under: 50 in Women, and 40 in Men
5.) Blood Pressure ≥ 130/85 - ANS Parameters to define the presence of metabolic syndrome.
Class I: HLA-A, HLA-B, HLA-C Class II: HLA-DR - ANS Major Histocompatibility Complex
Antigens:
Class I & Class II
Accumulation of abnormal
inorganic acids, associated with diabetic ketoacidosis
1 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
,Excessive loss of bicarbonate, as in severe diarrhea
Renal Failure - ANS Metabolic Acidosis Caused By:
pH: Decreased
pCO2: Decreased - ANS Metabolic Acidosis Blood Gas Results:
Erythroid Value = Sum of all Nucleated RBC Precursors
Myeloid Value = Sum of Precursor Neutrophils, Eosinophils, and Basophils
*Lymphocytes and Monocytes are NOT counted*
Interpreted as overall Bone Marrow Cellularity - ANS Myeloid to Erythroid Ratio- (M:E) Ratio:
Myoglolbin - ANS Earliest Biochemical Marker for Myocardial Infraction
Lectin from the seeds of Dolichos Biflorus - ANS Anti-A1 Reagent is Made from What?
Rickettsia Rickettsii - ANS Rocky Mountain Spotted Fever is Transmitted by a Tick Infected
with what?
Oligopeptide in the blood that cause vasoconstriction of arterioles, increases blood pressure
and releases aldosterone. - ANS Angiotensin 2
Calcium and Phosphate - ANS Parathyroid Hormone Regulates what in the Body
Non-Pathogenic - ANS Barr Bodies are Classified as what?
2 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
,Interferon Gamma - ANS Cytokine most important for Increased Macrophage Cell Activity?
28 Days, unless the unit expires sooner. - ANS Shelf Life for Irradiated Blood?
MCV= 80-100 fL.
MCH= 27-31 pg.
MCHC= 32-36% - ANS Normal Ranges:
MCV
MCH
MCHC
MCV= (Hematocrit % / RBC's) x 10
MCH= (Hemoglobin / RBC's) x 10
MCHC= (Hemoglobin / Hematocrit) x 100 - ANS Formulas for:
MCV
MCH
MCHC
Muscle Mass - ANS Creatine Excretion Correlates be with:
37o C for 1 hour - ANS If not tested right away Semen should be stored:
Light Spin, Followed by a Hard Spin - ANS Procedure for preparation of Platelet concentrate
from whole-blood:
3 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
, Transferrin Saturation, due to its sensitivity and specificity for iron overload - ANS Best way to
test for Hereditary Hemochromatosis:
Positive QC: Pseudomonas Aeruginosa
Negative QC: Escherichia Coli - ANS Best Stock Cultures to perform QC testing of oxidase
production?
Middle Aged Adults - ANS What age group is Hereditary Hemochromatosis usually detected?
It is caused by the engrafting of immunocompetent T lymphocytes into a severely
immunosuppressed recipient. They can be prevented by gamma irradiation of cellular blood
components. - ANS What is responsible for causing Graft-Versus-Host Reactions?
Forward Scatter: Size
Side Scatter: Granularity - ANS In Flow Cytometry, Forward Scatter and Side Scatter tell us
what about the cells?
Eosinophils. Crystals have a strong association with parasitic infections or allergic reactions -
ANS Charcot-Leyden Crystals in stool are breakdown products of what?
Used to calculate LDL Cholesterol.
LDL = [Total Cholesterol] - [HDL] - [Triglyceride/5] - ANS What is the Friedewald Formula, and
what does it calculate?
Megoblastic Anemia, and Lead Poisoning - ANS What Diseases are Cabot Rings associated
with?
4 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
