CHEMISTRY COMPREHENSIVE EXAM QUESTIONS AND CORRECT ANSWERS

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CHEMISTRY COMPREHENSIVE EXAM QUESTIONS AND CORRECT ANSWERS

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2025/2026

Institution Analytical Chemistry

Course Analytical Chemistry

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CHEMISTRY COMPREHENSIVE EXAM QUESTIONS AND CORRECT ANSWERS

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CHEMISTRY COMPREHENSIVE EXAM
QUESTIONS AND CORRECT ANSWERS
TheA2glycosylatedA2hemoglobinA2valueA2representsA2aA2timeA2averageA2ofA2glucoseA2con
centrationA2duringA2theA2preceding:A2-A2Ans---8A2toA212A2weeks

GivenA2theA2followingA2results:

AlkalineA2phosphataseA2SlightA2increaseA2
AminotransferaseA2MarkedA2increase
AspartateA2aminotransferaseA2MarkedA2increase
AlanineA2gamma-glutamylA2transferaseA2SlightA2increase

ThisA2isA2mostA2consistentA2with:A2-A2Ans---AcuteA2hepatitis

TheA2reasonA2carbonA2monoxideA2isA2soA2toxicA2isA2becauseA2it:A2-A2Ans---
hasA2200A2timesA2theA2affinityA2ofA2oxygenA2forA2hemoglobinA2bindingA2sites

AA2trueA2statementA2aboutA2highA2performanceA2liquidA2chromatographyA2(HPLC)A2isA2tha
tA2it:A2-A2Ans---
canA2beA2usedA2forA2absorbtion,A2partition,A2ionA2exchange,A2andA2gelA2permeationA2chro
matography

InA2theA2internationalA2systemA2ofA2unitsA2serumA2ureaA2isA2expressedA2inA2millimolesA2pe
rA2liter.A2AA2serumA2ureaA2nitrogenA2concentrationA2ofA228A2mg/
dLA2wouldA2beA2equivalentA2toA2whatA2concentrationA2ofA2urea?
(Urea:A2NH2CONH2A2atomicA2wtA2NA2=A214,A2CA2=A212,A20=A216,A2HA2=A21)A2-A2Ans---
10.0A2mEq/L

AA248A2yearA2oldA2womanA2withA2mildA2jaundiceA2hasA2theA2followingA2serumA2enzymeA2re
sults:AlkalineA2phosphataseA2(untreated)A2=A2600U/LA2(adults:A21-150A2U/L)Gamma-
glutamylA2transferaseA2=A2230A2U/LA2(normal:A20A2-A255A2U/
L)AlkalineA2phosphatase(afterA210A2mins.A2incubationA2atA256A2degreesA2C)A2=A2300A2U/
LA2(50%A2ofA2originalA2activity)ASTA2=A2400A2U/LA2(normal:A20A2-A250A2U/
L)WhichA2ofA2theA2followingA2sourcesA2mostA2contributesA2toA2theA2patient'sA2serumA2alkal
ineA2phosphataseA2activity?A2-A2Ans---Liver

AsparateA2aminotransferaseA2(AST)A2isA2characteristicallyA2elevatedA2inA2diseasesA2ofA2t
he:A2-A2Ans---Liver

NinetyA2percentA2ofA2theA2copperA2presentA2inA2theA2bloodA2isA2boundA2to:A2-A2Ans---
Ceruloplasmin

, IfA2theA2totalA2bilirubinA2isA24.3A2mg/dLA2andA2theA2conjugatedA2bilirubinA2isA22.1A2mg/
dL,A2theA2unconjugatedA2bilirubinA2is:A2-A2Ans---2.2A2mg/dL

TheA2selectivityA2ofA2anA2ion-selectiveA2electrodeA2isA2determinedA2byA2the:A2-A2Ans---
propertiesA2ofA2theA2membraneA2used

WhichA2ofA2theA2followingA2serumA2proteinsA2migratesA2withA2theA2beta-
globulinsA2onA2celluloseA2acetateA2atA2pHA28.6?A2-A2Ans---
c3A2componentA2ofA2complement

InA2aA2double-beamA2photometer,A2theA2additionalA2beamA2isA2usedA2to:A2-A2Ans---
correctA2forA2variationsA2inA2lightA2sourceA2intensity

InA2aA2spectrophotometer,A2lightA2ofA2aA2specificA2wavelengthA2canA2beA2isolatedA2fromA2
whiteA2lightA2withA2a(n):A2-A2Ans---DiffractionA2grating

ToA2ensureA2anA2accurateA2ammoniaA2levelA2result,A2theA2specimenA2shouldA2be:A2-
A2Ans---Spun,A2separated,A2iced,A2andA2testedA2immediately

SerumA2fromA2aA2patientA2withA2metastaticA2carcinomaA2ofA2theA2prostateA2wasA2separate
dA2fromA2theA2clotA2andA2storedA2atA2roomA2temperature.A2TheA2followingA2resultsA2wereA2
obtained:
PatientA2ReferenceA2Range
Calcium:A210.8A2mg/dLA28.8-10.3A2mg/dL
LD:420A2U/LA250-150A2U/L
AcidA2phosphataseA2:0.1A2U/LA20-5.5A2U/L

TheA2technicianA2shouldA2repeatA2the:A2-A2Ans---
AcidA2phosphataseA2withA2freshlyA2drawnA2serum

WhichA2ofA2theA2followingA2statementsA2aboutA2fluorometryA2areA2true?A2-A2Ans---
AA2compoundA2isA2saidA2toA2fluoresceA2whenA2itA2absorbsA2lightA2atA2oneA2wavelengthA2a
ndA2emitsA2lightA2atA2aA2secondA2wavelength.

TheA2directionA2inA2whichA2albuminA2migratesA2(ie,A2towardA2anodeA2orA2cathode)A2during
A2electrophoreticA2separationA2ofA2serumA2proteins,A2atA2pHA28.6,A2isA2determinedA2by:A2-
A2Ans---theA2ionizationA2ofA2theA2carboxylA2groups,A2yieldingA2aA2netA2negativeA2charge

BloodA2samplesA2wereA2collectedA2atA2theA2beginningA2ofA2anA2exerciseA2classA2andA2afte
rA230A2minutesA2ofA2aerobicA2activity.A2WhichA2ofA2theA2followingA2wouldA2beA2mostA2consi
stentA2withA2theA2postexerciseA2sample?A2-A2Ans---
elevatedA2lacticA2acid,A2elevatedA2pyruvate

TheA2mostA2consistentA2analyticalA2errorA2involvedA2inA2theA2routineA2determinationA2ofA2
HDLcholesterolA2isA2causedA2by:A2-A2Ans---aA2smallA2concentrationA2ofA2APO-
BA2containingA2lipoproteinsA2afterA2precipitation

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