MCB 2000 Kinetics Lab Quiz and Answers .

What enzyme are we using in this lab? - Answer Alkaline phosphatase What is the purpose of an enzyme? - Answer To lower the activation energy What substrate are we using in this lab? - Answer p-nitrophenyl phosphate Alkaline phosphate does what? and works at what pH? - Answer Removes a phosphate group and works best at high pH What is Vmax? - Answer Maximum velocity of reaction Units for Vmax? - Answer M/sec What is Km? - Answer Michaelis constant Units for Km? - Answer Moles or concentration unit What is the mathematical equation for determining Km? - Answer Vmax/2 What is the Michaelis-Menton Equation? - Answer V0 = Vmax [S] / Km + [S] What is Kcat? - Answer The turnover number of an enzyme is the number of substrate molecules (or moles of substrate) that are converted to products per unit time, when the enzyme is fully saturated with the substrate What is Kcat/Km - Answer A large ratio favors the formation of products and small ratio favors formation of reactants What is the term for Kcat/Km? - Answer Catalytic efficiency Is it easy or hard to accurately find a value for Vmax when using Michaelis-Menton plot? - Answer Hard