[GPHT] MTLE Must-knows UPDATED
ACTUAL Exam Questions and CORRECT
Answers
Nitric acid - CORRECT ANSWER - most common and fastest decalcifying agent
Perenyi's fluid - CORRECT ANSWER - Decalcifying agent and tissue softener
Phloroglucin-Nitric acid - CORRECT ANSWER - Most rapid decalcifying agent
Composition of Perenyi's Fluid - CORRECT ANSWER - 10% Nitric acid
0.5% Chromic acid
Absolute ethyl alcohol
Hydrochloric acid - CORRECT ANSWER - Recommended for surface decalcification of
tissue block
Composition of Von Ebner's - CORRECT ANSWER - Sodium chloride
Hydrochloric acid
Distilled water
Chromic acid (Flemming's fluid) - CORRECT ANSWER - Used as a fixative and a
decalcifying agent
EDTA (Versene) - CORRECT ANSWER - most common chelating agent in the market
Ion exchange resins - CORRECT ANSWER - hastens decalcification by removing calcium
ions from formic acid containing decalcifying solutions
,Composition of electrolytic decalcification - CORRECT ANSWER - 88% Formic acid
Conc. HCl
Distilled H2O
Chemical method (Calcium oxalate test) - CORRECT ANSWER - Recommended method
for routine decalcification
Tissue softeners - CORRECT ANSWER - Perenyi's fluid
4% aqueous phenol solution
Molliflex
2% HCl or 1% HCl in 70% alcohol
Less than 10x - CORRECT ANSWER - Whatever dehydrating agent is used, amount
should not be ____ the volume of the tissue
Ethyl alcohol - CORRECT ANSWER - most commonly used and best dehydrating agent
Methyl alcohol - CORRECT ANSWER - toxic dehydrating agent
Isopropyl alcohol - CORRECT ANSWER - used in microwave processing schedules
Butyl alcohol - CORRECT ANSWER - for plant & animal microtechniques
Acetone - CORRECT ANSWER - raw material in making methampetamine
Dioxane (Diethylene dioxide) - CORRECT ANSWER - excellent dehydrating and clearing
agent
, Dioxane (Diethylene dioxide) - CORRECT ANSWER - formed peroxide may explode
upon air exposure and cumulatively toxic and carcinogenic
Tetrahydrofuran - CORRECT ANSWER - Dehydrant and clearing agent also miscible to
water and paraffin which can cause eye and skin irritations
Ethanol and Paraffin wax - CORRECT ANSWER - Clearing is a process wherein alcohol
is removed replaced with a solvent that is fully miscible with?
Xylene
Dioxane
Chloroform
Cedarwood oil - CORRECT ANSWER - Most commonly used clearing agents for
dealcoholization in the embedding process
Xylene - CORRECT ANSWER - Most commonly used clearing agent
Xylene - CORRECT ANSWER - Most rapid clearing agent
milky - CORRECT ANSWER - Xylene becomes ______ when an incompletely
dehydrated tissue is immersed in it
Chloroform - CORRECT ANSWER - clearing agent for tough tissues (skin, fibrinoid,
decalcified tissues), nervous tissue lymph nodes and embryos
Benzene - CORRECT ANSWER - Excessive exposure may be extremely toxic and may be
carcinogenic
Aplastic anemia - CORRECT ANSWER - Benzene may damage bone marrow and cause?
ACTUAL Exam Questions and CORRECT
Answers
Nitric acid - CORRECT ANSWER - most common and fastest decalcifying agent
Perenyi's fluid - CORRECT ANSWER - Decalcifying agent and tissue softener
Phloroglucin-Nitric acid - CORRECT ANSWER - Most rapid decalcifying agent
Composition of Perenyi's Fluid - CORRECT ANSWER - 10% Nitric acid
0.5% Chromic acid
Absolute ethyl alcohol
Hydrochloric acid - CORRECT ANSWER - Recommended for surface decalcification of
tissue block
Composition of Von Ebner's - CORRECT ANSWER - Sodium chloride
Hydrochloric acid
Distilled water
Chromic acid (Flemming's fluid) - CORRECT ANSWER - Used as a fixative and a
decalcifying agent
EDTA (Versene) - CORRECT ANSWER - most common chelating agent in the market
Ion exchange resins - CORRECT ANSWER - hastens decalcification by removing calcium
ions from formic acid containing decalcifying solutions
,Composition of electrolytic decalcification - CORRECT ANSWER - 88% Formic acid
Conc. HCl
Distilled H2O
Chemical method (Calcium oxalate test) - CORRECT ANSWER - Recommended method
for routine decalcification
Tissue softeners - CORRECT ANSWER - Perenyi's fluid
4% aqueous phenol solution
Molliflex
2% HCl or 1% HCl in 70% alcohol
Less than 10x - CORRECT ANSWER - Whatever dehydrating agent is used, amount
should not be ____ the volume of the tissue
Ethyl alcohol - CORRECT ANSWER - most commonly used and best dehydrating agent
Methyl alcohol - CORRECT ANSWER - toxic dehydrating agent
Isopropyl alcohol - CORRECT ANSWER - used in microwave processing schedules
Butyl alcohol - CORRECT ANSWER - for plant & animal microtechniques
Acetone - CORRECT ANSWER - raw material in making methampetamine
Dioxane (Diethylene dioxide) - CORRECT ANSWER - excellent dehydrating and clearing
agent
, Dioxane (Diethylene dioxide) - CORRECT ANSWER - formed peroxide may explode
upon air exposure and cumulatively toxic and carcinogenic
Tetrahydrofuran - CORRECT ANSWER - Dehydrant and clearing agent also miscible to
water and paraffin which can cause eye and skin irritations
Ethanol and Paraffin wax - CORRECT ANSWER - Clearing is a process wherein alcohol
is removed replaced with a solvent that is fully miscible with?
Xylene
Dioxane
Chloroform
Cedarwood oil - CORRECT ANSWER - Most commonly used clearing agents for
dealcoholization in the embedding process
Xylene - CORRECT ANSWER - Most commonly used clearing agent
Xylene - CORRECT ANSWER - Most rapid clearing agent
milky - CORRECT ANSWER - Xylene becomes ______ when an incompletely
dehydrated tissue is immersed in it
Chloroform - CORRECT ANSWER - clearing agent for tough tissues (skin, fibrinoid,
decalcified tissues), nervous tissue lymph nodes and embryos
Benzene - CORRECT ANSWER - Excessive exposure may be extremely toxic and may be
carcinogenic
Aplastic anemia - CORRECT ANSWER - Benzene may damage bone marrow and cause?
