Histotechnology questions with 100% correct answers rated A+

Microscope - correct answers Used to for the magnification of the tissues and cells. Microtome - correct answers Used for the cutting of the wax and tissues. Block holder (chuck) - correct answers Holds the tissue in position. Knife and Knife carrier - correct answers For actual cutting of tissue sections. Pawl, Ratcher Feed Wheel & Adjustment screw - correct answers Lines up the tissue block in proper position with knife, adjusting thickness of tissues for successive sections. Soft brush - correct answers After sectioning, all accumulated paraffin and tissues must be brushed away with a ______ Xylol - correct answers After carefully drying the machines and knife holder, the parts should be wiped with _____ Oiled - correct answers Movable portion should be ______ thoroughly to prevent rusting Covered - correct answers Always ______ when not in use. Rocking (Cambridge) Microtome - correct answers Invented by Paldwell Trefall in 1881. Rocking (Cambridge) Microtome - correct answers "Simplest" among types of microtomes Rocking (Cambridge) Microtome - correct answers For cutting serial sections of "LARGE BLOCKS" of paraffin embedded tissues. Rotary (Minot) Microtome - correct answers Invented by Minot . Rotary (Minot) Microtome - correct answers "Most common" type used for both routine and research Laboratories. Rotary (Minot) Microtome - correct answers For sectioning paraffin embedded sections. Sliding Microtome - correct answers Developed by Adams in 1789 Sliding Microtome - correct answers "Most dangerous" type of microtome due to its movable exposed knife. Sliding Microtome - correct answers For cutting celloidin-embedded tissues. Base sledge microtome & Standard sliding microtome - correct answers Two types of Sliding Microtome Freezing Microtome - correct answers Invented by Queckett in 1848. Freezing Microtome - correct answers Used to cut un-dehydrated tissues in frozen state (for unembedded frozen section. Cryostat/ Cold Microtome - correct answers Another name Rapid Frozen Section (RFS Cryostat/ Cold Microtome - correct answers Refrigerated apparatus used in "fresh tissue microtomy" Cryostat/ Cold Microtome - correct answers Most commonly used for "rapid preparation". Cryostat/ Cold Microtome - correct answers Adjustable thermostat at -5 to -30 degree Celsius Cryostat/ Cold Microtome - correct answers Usual type of microtome inside "Rotary Microtome" Ultra-thin Microtome - correct answers Used to produce sections "electron microscopy" - correct answers Tissues are embedded in "plastic resins" Glass and Diamond knives. - correct answers Knives used in Knives used Ultra-thin Microtome Glass Knives - correct answers semi-thin knives Diamond knives - correct answers semi-thin knives 0.5-1 micron - correct answers Thickness of semi-thin knives 500-1200 angstrom (50- 120nm - correct answers Thickness of Ultra-thin knives .Plane concave, Biconcave, Plane wedge - correct answers Types of conventional Steel Knives 25mm - correct answers Length of .Plane concave Plane concave - correct answers Use: for base sledge, rotary and rocking microtome. Plane concave - correct answers Less concave side: for Celloidin embedded tissue blocks Plane concave - correct answers More concave side: for Paraffin embedded tissue blocks. 120mm - correct answers Length of .Plane concave Biconcave - correct answers Both sides are concave for cutting paraffin embedded sections.