HTL – Carbohydrates questions with correct answers

Glycogen is most abundant in - Answer Liver, heart (cardiac muscle cells), womb Staining reactions for glycogen: - Answer PAS (+), diastase (sensitive), Bauer-feulgen (+), Best carmine (+) Which two glycogen fixatives preserves the most glycogen? - Answer Bouins & Rossman's Other glycogen fixatives - Answer 80% alcohol, alcoholic formalin (37% formalin, 95% alcohol), acetic alcohol formalin, aqueous solution (formalin) This type of fixative should be avoided for glycogen fixative due to? - Answer Mercuric chloride fixatives (zenkers and helly) Why is mercuric chloride avoided for glycogen Staining - Answer Produces an uneven glycogen staining What is the oldest method for carbohydrate staining? - Answer Iodine method Results of the iodine method - Answer Plant starch, amylose, amylopectin = blue, glycogen and amyloid = Red-brown Staining mechanism of this stain: positive reaction depends