BIOD 171 Lab 5 - notebook Portage learning

AH05 Testing bacteria for antibiotic sensitivity

Objective: To determine the threshold of antibiotic sensitivity across
bacterial strains using the Kirby-Bauer method.
(AKA standardized Disc Susceptibility Test)

Procedure
1. Streak bacteria A across an LB agar plate for confluent growth using a
sterile L-separator.
2. Evenly place the paper antibiotic discs on the plate.
3. Invert place and incubate at 30 degrees Celsius overnight.
4. Measure zones of inhibition (diameter).




m
er as
5. Compare results with sensitivity chart.




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eH w
Notes:




o.
Antibiotic Measurements
rs e (mm)
ou urc
Vancomycin 30
Clindamycin 16
o

Oxacillin 17
aC s
vi y re


Tobramycin 22
Erythromycin 24
Penicillin 36
ed d




Gentamicin 10
ar stu




Cefazolin 19
is




Results:
Th




 List diameter of zone inhibition for each disc.
 Reference chart to determine sensitivity level:
o Resistant > Intermediate > Susceptible
sh




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