ASCP SCYM STUDY QUESTIONS WITH CORRECT ANSWERS 2026

ASCP SCYM STUDY QUESTIONS WITH CORRECT ANSWERS
2026


Sample Processing - CORRECT ANSWERS -Optimized antibody concentration

Defined range of cell #

Define sample handling, time and temperature

Define acceptable time of delay for acquisition



CD2 - CORRECT ANSWERS -T cells: NK cells



CD3 - CORRECT ANSWERS -T cells



CD4 - CORRECT ANSWERS -T helper cells, monocytes (dimly expressed)



CD5 - CORRECT ANSWERS -T cells; Some B Cells



CD8 - CORRECT ANSWERS -T cytotoxic cells, macrophages



CD10 - CORRECT ANSWERS -Immature T, B Cells, PMN's



CD13 - CORRECT ANSWERS -Monocytes, PMN's



CD14 - CORRECT ANSWERS -Monocytes, PMN's (dimly expressed)



CD16 - CORRECT ANSWERS -NK cells, PMN's, monocytes

,CD19 - CORRECT ANSWERS -B Cells



CD20 - CORRECT ANSWERS -B Cells



CD25 - CORRECT ANSWERS -Activated T Cells



CD33 - CORRECT ANSWERS -Monocytes



CD34 - CORRECT ANSWERS -Stem/Progenitor Cells



CD45 - CORRECT ANSWERS -Leukocytes



CD56 - CORRECT ANSWERS -NK cells, monocytes (dimly expressed)



Anti-Kappa - CORRECT ANSWERS -B Cells



Anti-Lambda - CORRECT ANSWERS -B Cells



Tdt - CORRECT ANSWERS -Immature Lymphocytes



Monoclonal Antibody Staining Direct Staining - CORRECT ANSWERS -Rapid single step staining

Can use multiple antibodies from the same host



Monoclonal Antibody Staining Indirect Staining - CORRECT ANSWERS -Secondary amplifies
single

, A few labeled secondaries can detect many primaries



Clinical Applications of Flow - CORRECT ANSWERS --Instrument Performance

-Integrity of the Specimen

-Quality in Processing of Specimens

-Appropriate Reagents and Controls

-Qualified Technical Staff

-Regulatory requirements from CAP, TJC, CLIA, and the FDA exist to ensure the accurate
reporting of flow cytometric results.



Lymphocytes Subset Enumeration (Immunophenotyping) - CORRECT ANSWERS -2 Tubes: 1-
CD3/CD4/CD8/CD45

2- CD3/CD19/CD16&CD56/CD45

-May include analysis of CD4 and CD8 T Cells, B Cells and NK Cells.

-Results reported as proportion (%) of total lymphocyte population.



CD4 Counts in HIV Infection - CORRECT ANSWERS --Asses degree of immunodeficiency

-Diagnose of AIDS

-Institution of Anti-Retroviral (ARV) therapy and opportunistic infection (OI) prophylaxis

-Monitoring immune reconstitution



CD4 Immunophenotyping Panels - CORRECT ANSWERS -



2 color - CORRECT ANSWERS -Isotype Ctrl

CD45/CD14

CD3/CD4

CD3/CD8