ASCP CG (Classic Cytogenetics) Exam with Questions and Answers

ASCP CG (Classic Cytogenetics) Exam with Questions and Answers At 48hrs of exposure to PHA, what will be happening to T-lymphocytes? ANSWER Most of the mitoses observed are the result of one cell division. At 72hrs of exposure to PHA, what will be happening to T-lymphocytes? ANSWER Most cells have undergone 2 cell divisions. At 96hrs of exposure to PHA, what will be happening to T-lymphocytes? ANSWER Cells have exhausted media and begin to die. What is LPS? ANSWER A poly-clonal B-cell activator which can stimulate the division of B-lymphocytes. What is pokeweed? ANSWER A mitogen that stimulates both T- and B- cell lymphocytes. How do you test the sterility of culture media? ANSWER A small aliquot is placed into a 37 degree incubator overnight, and checked for turbidity the next day. What chemicals cause cell cycle synchronization? ANSWER Ethidium bromide, Actinomycin D, BrdU, MTX, FudR and excess thymidine. Which chemicals reduce chromosome contraction during G2 and M? ANSWER Ethidium bromide, Actinomycin D and BrdU.