Plasmid vectors Guides d'étude, Notes de cours & Résumés

m   1. Which of the following is the mRNA start codon in most cases? A. UAA B. AGU C. AUG D. UGA 2. Which of the types of RNA is the smallest? A. mRNA B. tRNA C. rRNA D. 3. Which of the following is not considered a pyrimidine? A. C B. T C. U D. G 4. Which of the following is paired correctly? A. A-G B. C-G C. A-U D. G-T 5. Which of the following characterizes a Western blot? A. Antibody/protein hybridization B. DNA/RNA combination C. RNA ...

MCB Exam 3 Questions & Answers 2023/2024 What is meant by recombinant DNA? - ANSWER-DNA w/fragments from 2+ sources Name the tools used to make a recombinant DNA. (mechanisms of gene cloning) - ANSWER-1) Isolate plasmid DNA from bacterial cell 2) Isolate DNA of interest 3) Insert gene of interest into plasmid DNA 4) Insert now recombinant DNA into bacterium 5) Culture bacteria to make copies Restriction enzymes - ANSWER-endonuclease that cuts DNA at specific sites Cloning vecto...

What is the purpose of having rrnBT4 in an expression vector? - correct answerEnhances the stability of the GOI transcript Why must reverse transcriptase be used to create a eukaryotic expression library? - correct answerThe resulting cDNAs do not contain non-protein coding introns Which type of ligation-independent cloning technique takes advantage of the recombination between lambda phage attB and attP sequences in the vectors and in the DNA fragment to be inserted? - correct answerGatew...

Bio 202 EXAM 3 QUESTIONS AND CORRECT DETAILED ANSWERS How is recombinant DNA technology used to clone the human GH gene? 1st step! - ANSWER 1. use PCR to obtain the gene (Simply cutting the DNA with restriction enzymes would not be an effective way to isolate a specific gene. The genome would be cut into so many fragments, if you ran a gel it would be a smear! Which fragment is yours? You would need hybridization to isolate your fragment. Wouldn't have much DNA- not like the amo...

Bio 202 EXAM 3 QUESTIONS AND CORRECT DETAILED ANSWERS How is recombinant DNA technology used to clone the human GH gene? 1st step! - ANSWER 1. use PCR to obtain the gene (Simply cutting the DNA with restriction enzymes would not be an effective way to isolate a specific gene. The genome would be cut into so many fragments, if you ran a gel it would be a smear! Which fragment is yours? You would need hybridization to isolate your fragment. Wouldn't have much DNA- not like the amo...

Creating DNA Libraries -Collections of cloned DNA fragments from a particular organism contained in plasmid vectors within host bacteria -Screen library to pick out different genes of interest Genomic DNA Libraries -Used to locate fragment with DNA of interest -Chromosomal DNA from tissue of interest is isolated and digested using a restriction enzyme (sticky/blunt ends) -Vector also digested -DNA ligase used to ligate chromosomal DNA fragment inside vector DNA (plasmids) -Recombinant ...

Molecular Basis of Disease Unit 4 Verified Questions And Answers A+ spread of diseases (4 types) - ANSWER-1. endemic- constant presence/and or visual prevalence of a disease or infectious agent in a population within a geographic area 2. sporadic- disease that occurs infrequently and irregularly 3. epidemic- an increase, often sudden, in # of cases of disease above what's expected in that population in that area (outbreak, but more limited in area) 4. pandemic- spread over several co...

AP Bio Genetics, RNA, and DNA Practice Exam 110 Questions with Verified Answers In his transformation experiments, what did Griffith observe? a. Mutant mice were resistant to bacterial infections. b. Mixing a heat-killed pathogenic strain of bacteria with a living nonpathogenic strain can convert some of the living cells into the pathogenic form. c. Mixing a heat-killed nonpathogenic strain of bacteria with a living pathogenic strain makes the pathogenic strain nonpathogenic. d. Infecti...

Bio 202 EXAM 3 QUESTIONS AND CORRECT DETAILED ANSWERS How is recombinant DNA technology used to clone the human GH gene? 1st step! - ANSWER 1. use PCR to obtain the gene (Simply cutting the DNA with restriction enzymes would not be an effective way to isolate a specific gene. The genome would be cut into so many fragments, if you ran a gel it would be a smear! Which fragment is yours? You would need hybridization to isolate your fragment. Wouldn't have much DNA- not like the amo...

Bio 202 EXAM 3 QUESTIONS AND CORRECT DETAILED ANSWERS How is recombinant DNA technology used to clone the human GH gene? 1st step! - ANSWER 1. use PCR to obtain the gene (Simply cutting the DNA with restriction enzymes would not be an effective way to isolate a specific gene. The genome would be cut into so many fragments, if you ran a gel it would be a smear! Which fragment is yours? You would need hybridization to isolate your fragment. Wouldn't have much DNA- not like the amo...