BTEC 6303 Final Exam 2023 with 100% correct answers

What is the purpose of having rrnBT4 in an expression vector? - correct answerEnhances the stability of the GOI transcript Why must reverse transcriptase be used to create a eukaryotic expression library? - correct answerThe resulting cDNAs do not contain non-protein coding introns Which type of ligation-independent cloning technique takes advantage of the recombination between lambda phage attB and attP sequences in the vectors and in the DNA fragment to be inserted? - correct answerGateway cloning "After cleavage of the formyl-methionine from the N-terminus of a peptide, certain amino acids remaining on the N-terminus will make the protein susceptible to proteases. Which are they? - correct answerF-phenylalanine L-leucine Y- Tyrosine W- Tryptophan What is true of alkaline phosphatase? -"In restriction cloning, both cloning vector and insert are treated with alkaline phosphatase" -Alkaline phosphatase promotes the formation of phosphodiester bonds between the restriction-enzyme digested plasmid ends -Alkaline phosphatase removes the phosphate group from the 3' ends of restriction-enzyme digested DNA strand -Alkaline phosphatase works on both strands of a cut DNA fragment -Alkaline phosphatase transfers a phosphate group from a sensor protein to a transcriptional response regulator - correct answer-Alkaline phosphatase works on both strands of a cut DNA fragment Having codons in the gene of interest that are not preferred in the expression host can lead to: - correct answeramino ac