SDS-page Study guides, Class notes & Summaries

ASCP MB TEST VERIFIED SOLUTIONS 100% PASS What is biotin and digoxigenin most commonly used for in the lab? Why are they safer? Probe labeling (aka tagging nucleic acids so that they can be visualized). They are safer because they are nonradioactive. Name the three basic methods used to label a DNA probe: 1. End Labeling 2. Nick Translation 3. Random priming What is SDS? Name 3 of its applications in the lab. Sodium Dodecyl Sulfate. A detergent. 1. Denatures proteins. 2. Used i...

Western blotting a. Proteins from SDS-PAGE are transferred to a nitrocellulose membrane using electrical current a. membrane is blocked so antibody will not bind nonspecifically b. Primary antibody is added and incubated with the membrane c. To detect antibodies that have bound to protein a secondary anti-immunoglobulin antibody coupled to a reporter a. Excess secondary antibody is washed off the membrane b. Substrate is added that precipitates upon reaction c. Reveals visible band where...

AAMC FL 1 Exam Questions and Answers 2024 If Reaction 2 (Figure 4) is repeated with HCl and the compound shown below, which of the following compounds is NOT a direct product (without rearrangement)? -Answer-Compound A requires a hydride shift to form- therefore not a direct product. NOT an SN2 reaction because of tertiary alcohol- it is SN1 What is the energy of the photons emitted by the LED at a frequency of 610 THz? (Note: h = 6.6 × 10-34 J·s) -Answer-The photon energy is the energy...

BACE Exam Practice Test Questions with Complete Solutions What is an example of a product easily found in nature? - ANSWER Antibiotic In a biotechnology lab, what is tap water is best used for? - ANSWER Washing glassware. What is the order of amino acids on a polypeptide chain encoded by? - ANSWER mRNA How do DNA molecules vary from one species to another? - ANSWER Number of genes and non-coding regions. What is agar? - ANSWER Solid media on ...

Gel electrophoresis separates molecules based on what?Changes in size, changes, and shape What side of the electrophoresis chamber does the DNA move and why?Towards the positive side because they are negatively charged What is the SDS-PAGE used today used to separate?denatured proteins During the gel electrophoresis, buffer solution is used to conduct electricity and maintain the pH. T or F?T To prepare .o% agarose gel in 50mL of 1X TAE buffer, how many grams of agarose is needed?.4g

Which part of a microscope is responsible for gathering diffuse rays from the microscope light source and illuminating the specimen with a small cone of bright light? - Condenser lens The magnification of a microscope is equal to.... - the product of magnification of the ocular times the objective lens The absorbance of UV or visible light by a compound and the subsequent release by that compound of some of the energy as longer wavelength (usually visible) light is known as - fluorescen...

basic units of life that compose all living things - Answer-Cells characteristics shared by all cells - Answer--Cells are composed of 4 major macromolecules -Genetic material is carried and stored in the form of DNA -DNA is transcribed into RNA and RNA is translated into proteins -Their appearance, behavior, and function is dictated by proteins -They reproduce on their own -Respond to stimuli -Adapt to their environment -Are highly organized -Maintain a constant internal environment Ho...

BBMB-301 Test questions and Answers for Final studying (Iowa State) Which class of biopolymers is responsible for separating distinct aqueous compartments within Eukaryotic cells? Lipids The ______ is surrounded by a double membrane and produces approximately 90% of the energy, in the form of ATP, used by a typical living cell Mitochondrion T or F: The central dogma in biochemistry describes the flow of information from RNA to DNA to polypeptide to functional protein False: its DNA to RN...

Which of the following is an example of a product easily found in nature? - Answer- Antibiotic In a biotechnology lab, tap water is best used for - Answer- washing glassware The order of amino acids on a polypeptide chain is encoded by - Answer- mRNA How do DNA molecules vary from one species to another? - Answer- Number of genes and non-coding regions What is agar? - Answer- Solid media on which bacteria grow What part of an amino acid differs from one to the other? - Answer- R g...

Worksheet 4: Attempt review My LMS Subjects / 2021-PSB-MED3LAB(SP-T3-PT) / Assessment / Worksheet 4 Started on Thursday, 28 October 2021, 12:05 AM State Finished Completed on Friday, 29 October 2021, 5:41 PM Time taken 1 day 17 hours Grade 19.50 out of 21.50 (91%) Information Laboratory Module 4 Assessment In this laboratory module, you prepared plasmid DNA (i.e. performed a “miniprep”) from overnight cultures of positive GFP-producing and negative non-GFP-producing transformed ce...