SDS-page Study guides, Class notes & Summaries

ACS BIOCHEMISTRY EXAM QUESTIONS AND ANSWERS ALL CORRECT Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANSWER Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exc...

how much BSA is needed to prepare 50 mL of 5 mg/ml of BSA (w/v)? - Answer-(50 mL)(5 mg,mL) = 250 mg a technician is assigned the task of inserting a gene, LanA, into a plasmid to ultimately be expressed as a therapeutic protein in E coli. label the following steps (1-4) in the correct order the technician must perform them to accomplish her task. - Answer-1) design primers to amplify the lanA sequence using polymerase chain reaction (PCR) and perform the PCR reaction 2) digest and provide ...

Henderson-Hasselbach Equation - pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - Separates sample based on size with smaller molecules eluting later. Ion-Exch...

FL 5 AAMC MCAT question with complete solution 2023What is the chemical structure of a component found in four of the five cofactors used by Na+-NQR? - correct answer says it is composed of flavin structure is a nucleotide base structure B could be structure C also could be structure D is an amino acid down between B and C to help, look at the captioned note on the table. it says Fl represents flavin which is the redox active center in four of the five cofactors of Na+-NQR C has an ubiquinone AN...

how much BSA is needed to prepare 50 mL of 5 mg/ml of BSA (w/v)? - Answer- (50 mL)(5 mg,mL) = 250 mg a technician is assigned the task of inserting a gene, LanA, into a plasmid to ultimately be expressed as a therapeutic protein in E coli. label the following steps (1-4) in the correct order the technician must perform them to accomplish her task. - Answer- 1) design primers to amplify the lanA sequence using polymerase chain reaction (PCR) and perform the PCR reaction 2) digest and provid...

QBM Exam 3 Questions With Complete Answers Graded A+ Which of the following DNA purification techniques CANNOT be used for genomic DNA purification? miniprep Which DNA purification technique involves purifying the sample using an ultracentrifuge? Cesium chloride gradient What technique is used to increase plasmid copy number and purify plasmids? Miniprep. Which of the following wavelengths is incorrectly matched? 240 nm - RNA concentration. The absorbance of a DNA sample at A260 = 1...

Albert's Chapter 8 Exam 115 Questions with Verified Answers WITTF: machine that sorts fluorescently labeled cells and nonlabeled cells into separate containers using a strong electric field - CORRECT ANSWER fluorescence-activated cell sorter How are antibodies used to fluorescently label cells? - CORRECT ANSWER fluorescent molecule attached to antibody, which binds to a very specific cell type How does a fluorescent-activated cell sorter work? - CORRECT ANSWER individual cells travel s...

Title: Analysis of the Conservation of S. cerevisiae MET2 Gene in S. pombe Throughout Evolution. Abstract S. cerevisiae and S. pombe are both members of the Ascomycota phylum. The two yeast species diverged from a common ancestor 1 billion years ago. A research project was conducted to investigate the conservation of the S. cerevisiae MET2 gene in S. pombe. The experiments utilized to evaluate MET2 conservation between the two species were spot plating, yeast colony PCR, restriction diges...

QBM Exam 3 Questions With Complete Answers Graded A+ Which of the following DNA purification techniques CANNOT be used for genomic DNA purification? miniprep Which DNA purification technique involves purifying the sample using an ultracentrifuge? Cesium chloride gradient What technique is used to increase plasmid copy number and purify plasmids? Miniprep. Which of the following wavelengths is incorrectly matched? 240 nm - RNA concentration. The absorbance of a DNA sample...

FL 5 AAMC MCAT Exam Questions and Answers 100% Verified & Updated What is the chemical structure of a component found in four of the five cofactors used by Na+-NQR? -Answer-says it is composed of flavin structure is a nucleotide base structure B could be structure C also could be structure D is an amino acid down between B and C to help, look at the captioned note on the table. it says Fl represents flavin which is the redox active center in four of the five cofactors of Na+-NQR C has...