HTL ASCP
University Of Mississippi Medical Center
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HTL (Histotechnologist) Bundle Set
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HTL (Histotechnologist) Bundle Set
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HTL ASCP Exam questions with correct answers
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Acetic Acid - Answer Non coagulant, penetrates quickly and leaves tissues very soft. Used often as an additive to precipitate and preserve the nucleoproteins and fix the nuclei. 
 
Acetic Acid - Answer Swells tissues dramatically by breaking collagen bonds and allowing bonds to absorb water. Used as additive to counter-act the shrinking effect of other fixatives. 
 
Formaldehyde - Answer Non coagulant and additive fixative that reacts with amino groups that link protein chains together....
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HTL ASCP Exam- Nerve questions with correct answers
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Cresyl Echt Violet (Luna) method - Answer Nissl substances (basophilic) dyed, alcohol used to differentiate. 
 
Cresyl Echt Violet (Vacca) method - Answer Nissl substances (basophilic) dyed, acetic acid used to differentiate. 
 
Bodain Method for Nerve Fibers - Answer Protargol (silver solution) with added COPPER destains connective tissue. Hydroquinone reduces, gold chloride, sodium thiosulfate follows.
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HTL ASCP Exam – Instrumentation questions with correct answers
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Polarizing Microscope - Answer Microscope used to identify crystals, talc, silica, or urates. 
 
Birefringence - Answer Transmitting light unequally in different directions- 
 
Phase-Contrast Microscope - Answer Microscope used to examine unstained objects. Not routinely used in histopath labs.
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HTL ASCP Exam – Processing questions with correct answers
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Dehydrating Agents - Answer Alcohol, dioxane, acetone, tetrahydrofuran. 
 
Clearing Agents - Answer Xylene, toluene, benzene, acetone, chloroform, cedarwood oil, tetrahydrofuran, dioxane, limonene, aliphatic hydrocarbons. 
 
Universal Solvent - Answer Solvents that perform both the dehydration and clearing steps. Example Dioxane, Tetrahydrofuran.