HTL ASCP Exam questions with correct answers

Acetic Acid - Answer Non coagulant, penetrates quickly and leaves tissues very soft. Used often as an additive to precipitate and preserve the nucleoproteins and fix the nuclei. Acetic Acid - Answer Swells tissues dramatically by breaking collagen bonds and allowing bonds to absorb water. Used as additive to counter-act the shrinking effect of other fixatives. Formaldehyde - Answer Non coagulant and additive fixative that reacts with amino groups that link protein chains together. Formaldehyde - Answer Greatest binding occurs between pH 7.5-8. Formaldehyde - Answer Preserves lipids but does not make them insoluble and can be lost during subsequent processing. Formaldehyde - Answer Does not preserve carbohydrates but stabilizes and fixes the proteins to trap glycogen. Formaldehyde - Answer Penetrates tissue quickly, fixes slowly and has the least shrinking effect any fixative. Formaldehyde - Answer Beside ethanol and acetone, this hardens tissue more than any other fixative. Microcrystalline dark brown pigment - Answer Formed