Maldi Samenvattingen, Notities en Examens

OL Lab 13: Protein Synthesis Learning Objectives  Explain the translation process from mRNA to amino acid  Explain post-translational modification of proteins  Explain protein synthesis processing in the ribosome  Explain the primary, secondary, tertiary and quaternary structures of protein  Explain the basic principles of mass spectrometry (MALDI-TOF) Introduction In the Protein Synthesis lab, you will learn about the difference between protein synthesis in prokaryote (u...

The advantages of using real-time PCR to detect Chlyamidia and Gonorrhea is... - Answer - allow detection of organisms that are hard to culture - can be automated for high volume processing MALDI-TOF improves traditional culture methods by... - Answer -reduces need for biochem tests -pathogen-same method for all bacteria and fungi -cheaper cost per sample Describe the precautions that the State lab takes to prevent contamination (3) - Answer -designated clean (pre) and dirty (pos...

How to calculate signal to noise - Answer-S/N = mean/STDEV -inverse of RSD -STDEV: 5 (#STDEV gaussian curve) -STDEV: peak-to-peak noise/5 S/N relationships - Answer--increase S/N, increase run time -decrease noise, increase S/N Beer's Law - AnswerBeer's Law Calculations - AnswerHard ionization vs soft ionization - Answer--hard: no M+ peak (or small) -soft: M+ peak is visible MS: hard ionization sources - Answer--impart high quantities of residual energy in the subject molecule invokin...

MMSC 438 EXAM 2 SET Q&A Most common STD - ANSWER Chlamydia (1.7 mil cases) Skin normal biota is coagulase-negative staph, cornybacterium, and microbacterium smegmatis - ANSWER males (all ages) Skin normal biota staph and corynbacterium - ANSWER females (prepuberty and post-menopause) Normal biota of the skin for reproductive-age females in some cases- they carry it asymptomatically - ANSWER GBS Normal biota of the skin for ___________ includes Enterobacteriaceae and anerobes, st...

Instrumental Analysis ACS Exam Questions With 100% Correct Answers How to calculate signal to noise - answerS/N = mean/STDEV -inverse of RSD -STDEV: 5 (#STDEV gaussian curve) -STDEV: peak-to-peak noise/5 S/N relationships - answer-increase S/N, increase run time -decrease noise, increase S/N Beer's Law - answer Beer's Law Calculations - answer Hard ionization vs soft ionization - answer-hard: no M+ peak (or small) -soft: M+ peak is visible MS: hard ionization sources - answer-impar...

Instrumental Analysis ACS Exam Questions With 100% Correct Answers How to calculate signal to noise - answerS/N = mean/STDEV -inverse of RSD -STDEV: 5 (#STDEV gaussian curve) -STDEV: peak-to-peak noise/5 S/N relationships - answer-increase S/N, increase run time -decrease noise, increase S/N Beer's Law - answer Beer's Law Calculations - answer Hard ionization vs soft ionization - answer-hard: no M+ peak (or small) -soft: M+ peak is visible MS: hard ionization sources - answer-i...

Most common STD - CORRECT ANSWER Chlamydia (1.7 mil cases) Skin normal biota is coagulase-negative staph, cornybacterium, and microbacterium smegmatis - CORRECT ANSWER males (all ages) Skin normal biota staph and corynbacterium - CORRECT ANSWER females (prepuberty and post-menopause) Normal biota of the skin for reproductive-age females in some cases- they carry it asymptomatically - CORRECT ANSWER GBS Normal biota of the skin for ___________ includes Enterobacteriace...

CMMB 461 FINALBasic components of mass spec - correct answer 1. Ion source 2. mass analyzer 3. detector Two main types of ionization - correct answer 1. Electrospray ionization 2. MALDI Electrospray Ionization (ES) - correct answer Peptide solution is passed through a narrow metal needle with a high electrical potential resulting in highly charged droplets of protonated peptide ions. Matrix-assisted laser desorption ionization (MALDI) - correct answer Sample is co-crystallized wi...

Chem 420 Exam Questions And Answers (Verified And Updated) Three types of electrical domains? - answerAnalog Signals - magnitude of voltage, current, charge, or power; continuous in both amplitude and time Time-domain Signals - time relationship of signal fluctuations; frequency, pulse width, phase Digital Info - Data encoded in discrete levels; great accuracy and precision Where does noise come from? (2 types) - answerChemical noise: temperature, pressure, humidity, fumes, etc Instrumen...

Chem 420 Exam Questions And Answers (Verified And Updated) Three types of electrical domains? - answerAnalog Signals - magnitude of voltage, current, charge, or power; continuous in both amplitude and time Time-domain Signals - time relationship of signal fluctuations; frequency, pulse width, phase Digital Info - Data encoded in discrete levels; great accuracy and precision Where does noise come from? (2 types) - answerChemical noise: temperature, pressure, humidity, fumes, etc Instrumen...