Study guides, Class notes & Summaries

histology - ANSWER-study of tissues tissues - ANSWER-an assemblage of cells that function in unison to perform specific functions in the body epithelial (category of tissue) - ANSWER-cellular tissue that covers all exposed body surfaces, coats interior openings and body cavities. connective (category of tissue) - ANSWER-structural support, framework for communication, energy storage muscle (category of tissue) - ANSWER-movement (locomotion) neural (category of tissue) - ANSWER-car...

match the celsius temp w/ its common application in the lab setting. 4 degrees -20 degrees 37 60 -70 -150 - ANSWER-routine refrigeration of solution temp of cryostats incubator temp oven temp to melt paraffin storage of frozen sections temp to snap freeze the problem in the above image could be a result of: - ANSWER-aggressive trimming of the block what can be done to eliminate the problem seen in Image A? (unit exm 1) - ANSWER-trim blocks more slowly and select sections wi...

lysis or dissolving of cells by enzymatic action - ANSWER-Autolysis Breakdown of tissue by bacterial action - ANSWER-Putrefaction fixative classified as aldehyde - ANSWER-Formalin found in Bouin's fluid, is explosive and poisonous - ANSWER-Picric Acid good fixative for nuclei & gives great detail - ANSWER-Zenker's Fluid recommended for fixation of tissues for electron microscopy - ANSWER-Gluteraldehyde cytology fixative - ANSWER-95% ethanol fixative classified as oxidizing...

Resolution - ANSWER-Ability of a microscope to distinguish two objects as separate What do you call a measurement of 10^-6 m? - ANSWER-Micrometer, or micron Light Microscope - ANSWER-microscope that uses a beam of light passing through one or more lenses to magnify an object What resolving power does a light microscope have? - ANSWER-0.25 micrometers Types of Light microscopes (4) - ANSWER-- Simple (Dissecting) - Compound (What we use in lab to view slides regularly) - Dark Field (...

Five major groups of fixatives: - ANSWER-Aldehydes, Mercurials, Alcohols, Oxidizing agents, Picrates Formaldehyde (formalin) and glutaraldehyde are part of what fixation group? - ANSWER-Aldehydes Of the Aldehydes, which is better for immunohistochemisty and why? - ANSWER-Formaldehyde because it cross links the proteins without denaturation (aka does not harm the structure of the proteins). Of the Aldehydes, which is better for electron microscopy and why? - ANSWER-Glutaraldehyde because...

Explain the steps involved in tissue processing - ANSWER-1. Surgery 2. Fixation 3. Grossing 4. Fixation 5. Processing 6. Embedding 7. Staining 8. Cover-slipping Discuss the process of fixation - ANSWER-Stabilization of proteins to prevent autolysis and putrefaction. -Maintain proper relationship between cells and extracellular material (keep as "life-life" as possible) -15-20 times volume fixative of tissue -10% NBF most common fixative Describe the goal of tissue proces...

1.Nuclear pores: A)Combine to form a hexagonal structure B)Have 50nm in diameter C)Their number is not connected with cellular activity D)Nuclear lamina are connected to their inner rings E)A and C are correct F)B and D are correct - ANSWER-E)A and C are correct The conducting zone of the respiratory system: A) Includes the upper airways, trachea and the bronchial tree (including the terminal bronchioles) B) Is designed for airflow and respiration C) Includes only the nose and the ph...

Oxytocin and antidiuretic hormone are two of the hormones secreted by the - ANSWER-Pituitary The primary antibody must be diluted 1:100 for an immunohistochemical procedure. How many micro liters of the primary antibody would be required to prepare a total volume of 2mL? - ANSWER-240 How many grams of NaOH (MW=40) are required to prepare a liter of 1N NaOH? - ANSWER-40 A one normal solution is equivalent to: - ANSWER-1 gram-equivalent weight of solute per 1L of solution (g/L) The num...

T/F To be characterized as a profession, considerable specialized training must be required. - ANSWER-T Histology is the study of - ANSWER-tissue T/F The purpose of having a procedure for specimen receipt is to monitor the specimen and the accompanying paperwork. - ANSWER-T T/F Accessioning is giving a specimen a common number, one that is not unique to the patient. - ANSWER-f T/F The tasks performed by the histotechnologist require patience, mechanical ability, knowledge of biology,...

Acetic acid - ANSWER-This fixative does not fix or destroy carbohydrates, and it does not fix lipids. It penetrates very rapidly and leaves tissue very soft. It is added to many fixative mixtures because of its ability to fix nuclei. Glutaraldehyde - ANSWER-Techniques using Schiff reagent, such as the periodic acid-Schiff (PAS) stain, cannot be used on __________ fixed tissues because false positive results will be obtained. Glyoxal - ANSWER-This fixative is the smallest dialdehyde and is ...