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Exam (elaborations)
BIOSCI 201 mid term test prep Exam questions and 
answers Graded A+ 
Co-translational translocation -Protein enters the ER while it is still being 
made by the ribosome; uses SRP and happens on the rough ER. 
Post-translational translocation -Protein is f
NewBIOSCI 201 mid term test prep Exam questions and 
answers Graded A+ 
Co-translational translocation -Protein enters the ER while it is still being 
made by the ribosome; uses SRP and happens on the rough ER. 
Post-translational translocation -Protein is fully made in the cytosol first 
and then transported into an organelle using chaperones. 
Difference: timing (co vs post) -Co-translational happens during 
translation, post-translational h
Exam (elaborations)
BIOSCI 201 Module A Exam questions and answers 
Graded A+ 
Transmission Electron Microscopy (TEM) -High-resolution imaging 
technique using electrons to visualise cell structures like ribosomes 
Cell Culture -Growing cells outside the organism for experim
PopularBIOSCI 201 Module A Exam questions and answers 
Graded A+ 
Transmission Electron Microscopy (TEM) -High-resolution imaging 
technique using electrons to visualise cell structures like ribosomes 
Cell Culture -Growing cells outside the organism for experimental study 
Primary Cells -D
Package deal
ANA Pictures Clinical Immunology Exam questions and answers
NewANA Pictures Clinical Immunology Exam questions and answers
Exam (elaborations)
Biosci 201 - Module 1 Exam questions and answers 
Graded A+ 
Where does post translational translocation occur -cytosolic proteins 
translated in cytosol (mitochondria, nucleus and chloroplast) 
Where does co-translational translocation occur? -Secretory
NewBiosci 201 - Module 1 Exam questions and answers 
Graded A+ 
Where does post translational translocation occur -cytosolic proteins 
translated in cytosol (mitochondria, nucleus and chloroplast) 
Where does co-translational translocation occur? -Secretory proteins 
translated on ribosomes attatched to RER (membrane, golgi and secreted) 
Pulse chase experiment -radiolabelled aa are incorporated into new 
proteins and chased with normal aa. Proteins were seen to attach to ER. 
Cell fractination -Ho...
Exam (elaborations)
Biosci 201 - module 2 Exam questions and answers 
Graded A+ 
Griffiths experiment -genetic material could be transferred between dead 
bacteria and living bacteria 
how did griffiths experiment work -rough strain and heat killed have no 
previously killed
NewBiosci 201 - module 2 Exam questions and answers 
Graded A+ 
Griffiths experiment -genetic material could be transferred between dead 
bacteria and living bacteria 
how did griffiths experiment work -rough strain and heat killed have no 
previously killed the mouse however when combined there is a transformation 
and transfer of bacterial phenotype to become virulent 
Avery-McCarty-Mcleo
Exam (elaborations)
BIOSCI 201 - Module A Exam questions and 
answers Graded A+ 
Translation on attached RER 
Targeted to PM for secretion 
Translation on free ribosomes 
targeted to cytosol 
cell fractionalization 
seperation of parts depending on size and density 
Readout
NewBIOSCI 201 - Module A Exam questions and 
answers Graded A+ 
Translation on attached RER 
Targeted to PM for secretion 
Translation on free ribosomes 
targeted to cytosol 
cell fractionalization 
seperation of parts depending on size and density 
Readout experiment 
In vitro translation of IgG mRN
Exam (elaborations)
BIOSCI 201 - Molecular biology - The central dogma 
and beyond Exam questions and answers Graded 
A+ 
Central dogma analogy 
Nucleus = reading room / blueprint (DNA) 
Cytoplasm = job site / photocopies (RNA) 
Cellular machine = protein 
RNA is the ...? of
NewBIOSCI 201 - Molecular biology - The central dogma 
and beyond Exam questions and answers Graded 
A+ 
Central dogma analogy 
Nucleus = reading room / blueprint (DNA) 
Cytoplasm = job site / photocopies (RNA) 
Cellular machine = protein 
RNA is the ...? of the
Exam (elaborations)
BIOSCI 201 Exam questions and answers Graded 
A+ 
what determines mitotic spindle angle -PAR protein 
what happens if PAR 3 is overexpressed -no rotation 
what happens if no PAR 3 -wrong orientation 
out of nematode and
NewBIOSCI 201 Exam questions and answers Graded 
A+ 
what determines mitotic spindle angle -PAR protein 
what happens if PAR 3 is overexpressed -no rotation 
what happens if no PAR 3 -wrong orientation 
out of nematode and
Exam (elaborations)
Biosci 201 - module 2 Exam questions and answers 
Graded A+ 
Griffiths experiment -genetic material could be transferred between dead 
bacteria and living bacteria 
how did griffiths experiment work -rough strain and heat killed have no 
previously killed
NewBiosci 201 - module 2 Exam questions and answers 
Graded A+ 
Griffiths experiment -genetic material could be transferred between dead 
bacteria and living bacteria 
how did griffiths experiment work -rough strain and heat killed have no 
previously killed the mouse however when combined there is a transformation 
and transfer of bacterial phenotype to become virulent 
Avery-McCarty-Mcleod -isolated and degraded the transforming 
component 
isolation -obtained only a f
Exam (elaborations)
biosci 201 test prep (rosales) Exam questions and 
answers Graded A+ 
how is myocardium repaired - 
Cells of the immune response -T lymphocytes 
b lymphocytes 
natural killer cells 
macrophages 
dendritic cells- alert lymphocytes to presence of stuff that
Newbiosci 201 test prep (rosales) Exam questions and 
answers Graded A+ 
how is myocardium repaired - 
Cells of the immune response -T lymphocytes 
b lymphocytes 
natural killer cells 
macrophages 
dendritic cells- alert lymphocytes to presence of stuff that's foreign 
reticular cellsTunics of Blood Vess
Exam (elaborations)
biosci 201 RD Exam questions and answers Graded 
A+ 
What is normal cell fate? 
They have restricted cell fates. Their proliferation and differentiation is tightly 
regulated, ending in long-term senescence or death. Cells are also constrained to 
a parti
Newbiosci 201 RD Exam questions and answers Graded 
A+ 
What is normal cell fate? 
They have restricted cell fates. Their proliferation and differentiation is tightly 
regulated, ending in long-term senescence or death. Cells are also constrained to 
a particular site in a particular tissue (strong adhesion to neighbouring cells). 
What are the characteristics of cancer cells? 
- uncontrolled proliferation: autonomous growth signalling (gain of function of 
oncogenes), lack of gro
Exam (elaborations)
biosci 201 notes Exam questions and answers 
Graded A+ 
co-translational translocation (6) e.g insulin, enzymes, growth factos, antibodies. 
collagen, venom -1. N' signal sequence (hydrophobic, ~6-12 amino acids) 
2. Signal recognition particle (SR
Newbiosci 201 notes Exam questions and answers 
Graded A+ 
co-translational translocation (6) e.g insulin, enzymes, growth factos, antibodies. 
collagen, venom -1. N' signal sequence (hydrophobic, ~6-12 amino acids) 
2. Signal recognition particle (SRP) binds the sequence and the ribosome (halts 
translation) 
3. SRP targets the complex to the ER by binding the SRP receptor in the 
membrane, GTP binding 
4. SRP receptor subu
Exam (elaborations)
biosci 201 module B lecrture 3 and 4 Exam 
questions and answers Graded A+ 
What are the three main processes involved in mRNA processing? 
Transcription, mRNA processing, and mRNA translation. 
What is the role of RNA Polymerase II (RNA Pol II) in mRNA p
Newbiosci 201 module B lecrture 3 and 4 Exam 
questions and answers Graded A+ 
What are the three main processes involved in mRNA processing? 
Transcription, mRNA processing, and mRNA translation. 
What is the role of RNA Polymerase II (RNA Pol II) in mRNA processing? 
RNA Pol II is responsible for transcribing mRNA and hosts enzymes for capping, 
splicing, cleavage, and polyadenylation. 
What is the significance of the 5′ cap on mRNA? 
The 5′ cap protects the RNA from degradation, masks the 5...
Exam (elaborations)
BIOSCI 201 Module B Exam questions and answers 
Graded A+ 
Gene Expression: 
What is gene expression? -The process of making RNA and protein from a 
gene 
Gene Expression: 
Why do different cells express different genes? -Because only specific 
genes are
NewBIOSCI 201 Module B Exam questions and answers 
Graded A+ 
Gene Expression: 
What is gene expression? -The process of making RNA and protein from a 
gene 
Gene Expression: 
Why do different cells express different genes? -Because only specific 
genes are transcri
Exam (elaborations)
biosci 201 lecture 5 and 6 module A Exam 
questions and answers Graded A+ 
What is the role of lysosomal proteins? 
They break down macromolecules into monomer building blocks and recycle 
organelles. 
What modification serves as the lysosomal sorting sig
Newbiosci 201 lecture 5 and 6 module A Exam 
questions and answers Graded A+ 
What is the role of lysosomal proteins? 
They break down macromolecules into monomer building blocks and recycle 
organelles. 
What modification serves as the lysosomal sorting signal? 
Mannose-6-phos
Exam (elaborations)
biosci 201 lecture 3 and 4 module A Exam 
questions and answers Graded A+ 
What are COP I and COP II coated vesicles? 
COP I and COP II are types of vesicles that transport proteins between the 
endoplasmic reticulum (ER) and the Golgi apparatus. 
What is
Newbiosci 201 lecture 3 and 4 module A Exam 
questions and answers Graded A+ 
What are COP I and COP II coated vesicles? 
COP I and COP II are types of vesicles that transport proteins between the 
endoplasmic reticulum (ER) and the Golgi apparatus. 
What is the primary function of COP I vesicles? 
COP I vesicles are involved in retrograde transport, moving proteins back to the 
ER. 
What initiates the formation of COP II vesicles? 
COP II vesicles are initia
Exam (elaborations)
biosci 201 lecture 1 and 2 module 1 Exam 
questions and answers Graded A+ 
What does the signal hypothesis address? 
It addresses how proteins are targeted to different parts of the cell. 
What are the two main methods of protein targeting in eukaryotic c
Newbiosci 201 lecture 1 and 2 module 1 Exam 
questions and answers Graded A+ 
What does the signal hypothesis address? 
It addresses how proteins are targeted to different parts of the cell. 
What are the two main methods of protein targeting in eukaryotic cells? 
Post-translational translocation and co-translational translocation. 
Where are cytosolic proteins translated? 
In the cytosol. 
What type of proteins ar
Exam (elaborations)
biosci 201 JM Exam questions and answers Graded 
A+ 
Proteins of the immune system 
Cytokines, prostaglandins 
Organs of the immune system 
Bone marrow, thymus, lymph nodes, spleen 
4 key features of the immune system 
Tolerance - can distinguish self-ant
Newbiosci 201 JM Exam questions and answers Graded 
A+ 
Proteins of the immune system 
Cytokines, prostaglandins 
Organs of the immune system 
Bone marrow, thymus, lymph nodes, spleen 
4 key features of the immune system 
Tolerance - can distinguish self-antigens from foreign antigens. 
Diversity - can recognise & respond to many different molecules. 
Specificity - can distinguish
Exam (elaborations)
BIOSCI 201 immunology Exam questions and 
answers Graded A+ 
What is the immune system and what is its primary function? 
The immune system is your defence system. 
It distinguishes between self and non-self (or altered self) and defends the body 
against
NewBIOSCI 201 immunology Exam questions and 
answers Graded A+ 
What is the immune system and what is its primary function? 
The immune system is your defence system. 
It distinguishes between self and non-self (or altered self) and defends the body 
against infections (bacteria, viruses, fungi, parasites) and cancer, and heals 
damage to tissues. 
Define "antigen". 
An antigen is a molecule
Exam (elaborations)
biosci 201 HS Exam questions and answers Graded 
A+ 
Cleavage in C. elegans 
The embryo is rapidly cleaved into smaller and smaller cells called blastomeres. At 
the two cell stage there are two cells AB (future anterior) and P1 (future 
posterior). 
Why
Newbiosci 201 HS Exam questions and answers Graded 
A+ 
Cleavage in C. elegans 
The embryo is rapidly cleaved into smaller and smaller cells called blastomeres. At 
the two cell stage there are two cells AB (future anterior) and P1 (future 
posterior). 
Why is cleavage so rapid in C. elegans? 
Early cleavage divisions don't depend on new gene activity from the zygotic DNA, 
instead, maternal p