HESI ADMISSION ASSESSMENT (A2) EXAM
(LATEST UPDATE 2026/2027) | QUESTIONS
AND ANSWERS | GRADE A | 100% CORRECT
(VERIFIED SOLUTIONS)
AT CHAMBERLAIN COLLEGE OF NURSING
HESI ADMISSION ASSESSMENT (A2)
BEST, SAFE STRUCTURE (RECOMMENDED):
Questions 1–25: Biology
Questions 26–75: Vocabulary
Questions 76–100: Anatomy & Physiology
Questions 101–150: Reading Comprehension
Questions 151–200: Math / Grammar / Mixed
SEQUENTIAL PARTS:
Part 1: Questions 1–25
Part 2: Questions 26–50
Part 3: Questions 51–75
Part 4: Questions 76–100
Part 5: Questions 101–150
Part 6: Questions 151–200
BIOLOGY (QUESTIONS 1–25)
BIOLOGY / CELL PHYSIOLOGY – QUESTIONS
1. Which movement requires a carrier protein but no direct cellular energy?
A. Active transport
B. Osmosis
C. Facilitated diffusion
D. Simple diffusion
,Correct Answer: C. Facilitated diffusion
Rationale:
Facilitated diffusion is a passive transport process that relies on carrier or channel proteins to move
substances across the cell membrane. The movement occurs down the concentration gradient, from high to
low concentration. Because no ATP is required, it is considered a form of passive transport. This mechanism
is especially important for polar molecules like glucose.
2. Which term denotes the movement of glucose molecules from an area of lower
concentration to an area of higher concentration?
A. Diffusion
B. Facilitated diffusion
C. Osmosis
D. Active transport
Correct Answer: D. Active transport
Rationale:
Active transport moves substances against their concentration gradient, from lower to higher concentration.
This process requires cellular energy in the form of ATP and uses specific carrier proteins. Glucose can be
actively transported in certain tissues such as the intestines and kidneys. Without energy, this movement
would not occur.
3. Plasmolysis is a term describing?
A. Cell swelling in hypotonic solution
B. Cell bursting in isotonic solution
C. Cellular shrinkage in hypertonic solution
D. Cell division
Correct Answer: C. Cellular shrinkage in hypertonic solution
Rationale:
Plasmolysis occurs when a cell is placed in a hypertonic solution and loses water through osmosis. As water
exits the cell, the plasma membrane pulls away from the cell wall. This process is commonly observed in
plant cells. It demonstrates the effects of osmotic pressure on cells.
4. The movement of substances from lesser concentration to higher concentration is called?
A. Diffusion
B. Facilitated diffusion
,C. Osmosis
D. Active transport
Correct Answer: D. Active transport
Rationale:
Active transport requires energy to move molecules against their concentration gradient. This mechanism is
essential for maintaining proper cellular conditions. Ion pumps such as the sodium-potassium pump are
classic examples. Without active transport, cells could not regulate internal ion balance.
5. Which particular structure is present in both eukaryotic and prokaryotic cells?
A. Nucleus
B. Mitochondria
C. Cell membrane
D. Endoplasmic reticulum
Correct Answer: C. Cell membrane
Rationale:
All living cells possess a cell membrane that encloses the cytoplasm. This membrane regulates the
movement of substances into and out of the cell. Prokaryotes lack membrane-bound organelles, but the
plasma membrane is always present. It is essential for cell survival.
6. Plant cells differ from animal cells in?
A. Presence of mitochondria
B. Presence of ribosomes
C. Presence of cell wall
D. Presence of nucleus
Correct Answer: C. Presence of cell wall
Rationale:
Plant cells possess a rigid cell wall made primarily of cellulose. This structure provides mechanical support
and protection. Animal cells lack cell walls and rely on the cytoskeleton for shape. The cell wall is a defining
feature of plant cells.
7. Which cell type lacks a true nucleus and membrane-bound organelles?
A. Eukaryotic cell
B. Plant cell
, C. Animal cell
D. Prokaryotic cell
Correct Answer: D. Prokaryotic cell
Rationale:
Prokaryotic cells, such as bacteria, do not have a membrane-bound nucleus. Their genetic material is found
in a nucleoid region. They also lack organelles like mitochondria and ER. This simpler structure
distinguishes them from eukaryotic cells.
8. Which organelle contains hydrolytic enzymes and is called the “suicide bag”?
A. Ribosome
B. Golgi apparatus
C. Lysosome
D. Peroxisome
Correct Answer: C. Lysosome
Rationale:
Lysosomes contain digestive enzymes capable of breaking down cellular waste. If these enzymes are
released, they can digest the cell itself. For this reason, lysosomes are sometimes called “suicide bags.” They
play a crucial role in intracellular digestion.
9. Pinocytosis is the process of?
A. Engulfing solid particles
B. Taking in liquids
C. Releasing substances
D. Active transport
Correct Answer: B. Taking in liquids
Rationale:
Pinocytosis is a form of endocytosis known as “cell drinking.” The cell membrane encloses extracellular
fluid and dissolved substances into vesicles. This process does not discriminate specific molecules. It helps
cells sample their environment.
10. A membrane that allows some substances to pass while restricting others is?
A. Impermeable
B. Permeable
(LATEST UPDATE 2026/2027) | QUESTIONS
AND ANSWERS | GRADE A | 100% CORRECT
(VERIFIED SOLUTIONS)
AT CHAMBERLAIN COLLEGE OF NURSING
HESI ADMISSION ASSESSMENT (A2)
BEST, SAFE STRUCTURE (RECOMMENDED):
Questions 1–25: Biology
Questions 26–75: Vocabulary
Questions 76–100: Anatomy & Physiology
Questions 101–150: Reading Comprehension
Questions 151–200: Math / Grammar / Mixed
SEQUENTIAL PARTS:
Part 1: Questions 1–25
Part 2: Questions 26–50
Part 3: Questions 51–75
Part 4: Questions 76–100
Part 5: Questions 101–150
Part 6: Questions 151–200
BIOLOGY (QUESTIONS 1–25)
BIOLOGY / CELL PHYSIOLOGY – QUESTIONS
1. Which movement requires a carrier protein but no direct cellular energy?
A. Active transport
B. Osmosis
C. Facilitated diffusion
D. Simple diffusion
,Correct Answer: C. Facilitated diffusion
Rationale:
Facilitated diffusion is a passive transport process that relies on carrier or channel proteins to move
substances across the cell membrane. The movement occurs down the concentration gradient, from high to
low concentration. Because no ATP is required, it is considered a form of passive transport. This mechanism
is especially important for polar molecules like glucose.
2. Which term denotes the movement of glucose molecules from an area of lower
concentration to an area of higher concentration?
A. Diffusion
B. Facilitated diffusion
C. Osmosis
D. Active transport
Correct Answer: D. Active transport
Rationale:
Active transport moves substances against their concentration gradient, from lower to higher concentration.
This process requires cellular energy in the form of ATP and uses specific carrier proteins. Glucose can be
actively transported in certain tissues such as the intestines and kidneys. Without energy, this movement
would not occur.
3. Plasmolysis is a term describing?
A. Cell swelling in hypotonic solution
B. Cell bursting in isotonic solution
C. Cellular shrinkage in hypertonic solution
D. Cell division
Correct Answer: C. Cellular shrinkage in hypertonic solution
Rationale:
Plasmolysis occurs when a cell is placed in a hypertonic solution and loses water through osmosis. As water
exits the cell, the plasma membrane pulls away from the cell wall. This process is commonly observed in
plant cells. It demonstrates the effects of osmotic pressure on cells.
4. The movement of substances from lesser concentration to higher concentration is called?
A. Diffusion
B. Facilitated diffusion
,C. Osmosis
D. Active transport
Correct Answer: D. Active transport
Rationale:
Active transport requires energy to move molecules against their concentration gradient. This mechanism is
essential for maintaining proper cellular conditions. Ion pumps such as the sodium-potassium pump are
classic examples. Without active transport, cells could not regulate internal ion balance.
5. Which particular structure is present in both eukaryotic and prokaryotic cells?
A. Nucleus
B. Mitochondria
C. Cell membrane
D. Endoplasmic reticulum
Correct Answer: C. Cell membrane
Rationale:
All living cells possess a cell membrane that encloses the cytoplasm. This membrane regulates the
movement of substances into and out of the cell. Prokaryotes lack membrane-bound organelles, but the
plasma membrane is always present. It is essential for cell survival.
6. Plant cells differ from animal cells in?
A. Presence of mitochondria
B. Presence of ribosomes
C. Presence of cell wall
D. Presence of nucleus
Correct Answer: C. Presence of cell wall
Rationale:
Plant cells possess a rigid cell wall made primarily of cellulose. This structure provides mechanical support
and protection. Animal cells lack cell walls and rely on the cytoskeleton for shape. The cell wall is a defining
feature of plant cells.
7. Which cell type lacks a true nucleus and membrane-bound organelles?
A. Eukaryotic cell
B. Plant cell
, C. Animal cell
D. Prokaryotic cell
Correct Answer: D. Prokaryotic cell
Rationale:
Prokaryotic cells, such as bacteria, do not have a membrane-bound nucleus. Their genetic material is found
in a nucleoid region. They also lack organelles like mitochondria and ER. This simpler structure
distinguishes them from eukaryotic cells.
8. Which organelle contains hydrolytic enzymes and is called the “suicide bag”?
A. Ribosome
B. Golgi apparatus
C. Lysosome
D. Peroxisome
Correct Answer: C. Lysosome
Rationale:
Lysosomes contain digestive enzymes capable of breaking down cellular waste. If these enzymes are
released, they can digest the cell itself. For this reason, lysosomes are sometimes called “suicide bags.” They
play a crucial role in intracellular digestion.
9. Pinocytosis is the process of?
A. Engulfing solid particles
B. Taking in liquids
C. Releasing substances
D. Active transport
Correct Answer: B. Taking in liquids
Rationale:
Pinocytosis is a form of endocytosis known as “cell drinking.” The cell membrane encloses extracellular
fluid and dissolved substances into vesicles. This process does not discriminate specific molecules. It helps
cells sample their environment.
10. A membrane that allows some substances to pass while restricting others is?
A. Impermeable
B. Permeable
