BIOL 3130 MIDTERM 1 EXAM STUDY SET WITH COMPLETE SOLUTIONS 100% VERIFIED LATEST UPDATE!!

BIOL 3130 MIDTERM 1 EXAM STUDY SET WITH COMPLETE SOLUTIONS 100% VERIFIED
LATEST UPDATE!!




What are the following subunits of an RNA Polymerase? - (ANSWER)1.) 2 large subunit = B & B'

2.) Sigma subunit

3.) 2 copies of Alpha subunit

4. Omega subunit



What are the sizes of the following RNA Polymerase? - (ANSWER)B = 150 KD

B' = 160 KD

Omega = 10 KD

sigma = 70 KD

Alpha = 40 KD



What is a holoenzyme? - (ANSWER)It is an enzyme that contains all of the subunits of a RNA Polymerase,
which allows effective transcription



What is the difference between Core polymerase and Holoenzyme? - (ANSWER)Core polymerase lacks a
subunit (ex: sigma); holoenzyme have all of the subunit that is required to function properly



What are the roles of sigma subunit in the RNA Polymerase? - (ANSWER)1.) Scans and detects the
promoter region

2.) It facilitates binding of the RNA Polymerase tightly onto the promoter region



Can transcription occur without sigma subunit in the RNA Polymerase? - (ANSWER)Yes, but it must bind
to nicks and gaps or non-specifically (not in RNA Polymerase binding sites)



What are promoters? - (ANSWER)Promoters are regions in the strand where RNA Polymerase binds to
and starts transcription (initiation phase)



During the experiment of using T4 (native and intact DNA template) and Calf thymus (nicked), why is
there a difference between the transcription activity of different types of RNA Polymerases? -