BIOL 1208 Fall 2025
Techniques In-Class Assignment
Name: Nathan Beall
Lab Partner Name(s): Jackson
Remember: Although you work with partners for lab experiments, written assignments must be completed
independently and answered in your own words. All text must be able to be processed in Turnitin, so do not
use screenshots, etc. See the syllabus for more information.
EXERCISE I: PIPETTES AND PIPETTING
1. Insert a photo of your completed Serological Pipettes Exercise here:
• Crop photo and resize to 2x3
2. Insert a photo of your completed Micropipette Exercise (card) here:
• Crop photo and resize to 2x3
3. What is the range limit of the following pipettes?
• P100 micropipette: 10-100 microliters
• P1000 micropipette: 100-1000 microliters
4. What are the purposes of the first and second stops of a micropipette?
• First: to get the exact volume of liquid.
• Second: to fully dispose of the liquid.
For Questions 5 & 6: Using the appropriate micropipette, dispense 250 µL of water onto the scale. Follow the scale
directions at your bench to Tare/Zero scale before dispensing water into weigh boat.
5. List micropipette (P100 or P1000) selected: P1000
6. Scale readout of volume in grams: 0.256 g
7. A lab technician dispenses liquid onto a scale and the readout volume is 0.05 grams. How many microliters was
their pipette set to?
• He likely had it set to 50 Microliters
EXERCISE II: SPECTROMETRY
8. If an object is orange, which colors are being absorbed (ROYGBV)? Transmitted?
• Absorbed: Red, Yellow, Green, Blue, and Violet.
• Transmitted: Orange
9. What is directly measured by a spectrometer?
• The lights wave lengths.
10. What is the purpose of a blank in spectrometry?
• The blank serves as a baseline to base the sample off of.
11. What are the units for absorbance? For concentration?
• Absorbance: ODU
• Concentration: M
A. COBALT CHLORIDE
12. Based only on your observation of the colors of the solutions, which cobalt chloride (CoCl2) sample was more
concentrated (A or B)? How do you know?
• Solution A was more concentrated because it had a higher calculated concentration.
13. Provide the absorbance values from the spectrometer reading for both solutions. Use the graph to visually
estimate the concentration. Use the standard curve equation to calculate the concentration (Remember, solve
1
Techniques In-Class Assignment
Name: Nathan Beall
Lab Partner Name(s): Jackson
Remember: Although you work with partners for lab experiments, written assignments must be completed
independently and answered in your own words. All text must be able to be processed in Turnitin, so do not
use screenshots, etc. See the syllabus for more information.
EXERCISE I: PIPETTES AND PIPETTING
1. Insert a photo of your completed Serological Pipettes Exercise here:
• Crop photo and resize to 2x3
2. Insert a photo of your completed Micropipette Exercise (card) here:
• Crop photo and resize to 2x3
3. What is the range limit of the following pipettes?
• P100 micropipette: 10-100 microliters
• P1000 micropipette: 100-1000 microliters
4. What are the purposes of the first and second stops of a micropipette?
• First: to get the exact volume of liquid.
• Second: to fully dispose of the liquid.
For Questions 5 & 6: Using the appropriate micropipette, dispense 250 µL of water onto the scale. Follow the scale
directions at your bench to Tare/Zero scale before dispensing water into weigh boat.
5. List micropipette (P100 or P1000) selected: P1000
6. Scale readout of volume in grams: 0.256 g
7. A lab technician dispenses liquid onto a scale and the readout volume is 0.05 grams. How many microliters was
their pipette set to?
• He likely had it set to 50 Microliters
EXERCISE II: SPECTROMETRY
8. If an object is orange, which colors are being absorbed (ROYGBV)? Transmitted?
• Absorbed: Red, Yellow, Green, Blue, and Violet.
• Transmitted: Orange
9. What is directly measured by a spectrometer?
• The lights wave lengths.
10. What is the purpose of a blank in spectrometry?
• The blank serves as a baseline to base the sample off of.
11. What are the units for absorbance? For concentration?
• Absorbance: ODU
• Concentration: M
A. COBALT CHLORIDE
12. Based only on your observation of the colors of the solutions, which cobalt chloride (CoCl2) sample was more
concentrated (A or B)? How do you know?
• Solution A was more concentrated because it had a higher calculated concentration.
13. Provide the absorbance values from the spectrometer reading for both solutions. Use the graph to visually
estimate the concentration. Use the standard curve equation to calculate the concentration (Remember, solve
1
