MGY 250 Unit 6 Quiz Questions with Correct Answers Latest Update 2025/2026

MGY 250 Unit 6 Quiz Questions with Correct Answers Latest Update 2025/2026 According to Dr. Roth, the bottleneck with genome sequencing in terms of personalized medicine is in: Interpreting genomes The cost of sequencing Sequencing technology is not advanced enough Not having enough genomes sequenced - Answers Interpreting genomes The Human Genome Project: Was created from Craig Venter's DNA alone Used many cell types Sequenced several individuals to generate a reference sequence Was a solely Canadian project - Answers Sequenced several individuals to generate a reference sequence The method most likely to be used to test for a pathogenic allele at a well-known location such as the BRCA1 gene would be: Whole Genome Sequencing Whole Exome Sequencing Genotyping RNA-sequencing - Answers Genotyping Oftentimes the result of an experiment is considered statistically significant if the p-value is less than 0.05 (0.05 is denoted alpha). For GWAS, the significance threshold is set to values more like 10-7. This is because: All statistical tests should set alpha to 10-7 GWAS is a very conservative test When you are doing so many tests, like with GWAS, the probability of finding a significant result by chance is very high, so the p-value is adjusted to avoid false negatives When you are doing so many tests, like with GWAS, the probability of finding a significant result by chance is very high, so the p-value is adjusted to avoid false positives - Answers When you are doing so many tests, like with GWAS, the probability of finding a significant result by chance is very high, so the p-value is adjusted to avoid false positives The data from GWAS: Indicate many suggestive loci that are part of the haplotype block associated with the variant Indicate a locus that is associated with the variant Reveal the variant that causes disease unambiguously Indicates rare variants associated with disease - Answers Indicate many suggestive loci that are part of the haplotype block associated with the variant Most 'functional variants', as described by Dr. Roth: Are beneficial Are not subject to natural selection Are deleterious Are neutral - Answers Are deleterious In a burden of mutation analysis, the mutational make-up of cases and controls is: Cases: functional mutations, controls: neutral mutations Cases: neutral mutations, controls: neutral + beneficial mutations Cases: neutral + functional mutations, controls: neutral mutations Cases: deleterious mutations, controls: neutral mutations - Answers Cases: neutral + functional mutations, controls: neutral mutations Sometimes, expressing the human version of a gene in yeast is sufficient for yeast growth when the native yeast copy of the gene is knocked out. If so, a putative disease variant in that human gene can be tested for functionality by expressing it as the only copy of that gene in yeast. This strategy is best classified as: Comparative genomics Trans-species complementation