ASCP Molecular Biology Certification Exam ,Most Recent Exam
|| 2025 /2026 Actual Complete Real Exam Questions With
verified Answers (Correct Answers) Already Graded A+ ,
Guaranteed success| Newest Exam | Just Released !!
STUDY GUIDE EXAM
Histone acetylation close to the gene will increase or
decrease expression? -
ANSWER-
increase
siRNAs complementary to the gene transcript will
increase or decrease
expression? - ANSWER-
decrease
Calculate the DNA concentration from the
following:
260=0.172 (D.F. 1:100) - ANSWER-860 ug/mL = .172 abs * 50
ug/mL * 100 DF
[DNA] = 1535 ug/mL. You have 0.5 mL. What is the total yield.
- ANSWER-
767.5 ug = 1535 ug/mL *
0.5Ml
,[DNA] = 767.5 ug/mL. You have 0.5 mL What is the total yield.
- ANSWER-
383.75 ug = 767.5 ug/mL *
0.5 mL
[DNA] = 860 ug/mL. You have 0.5 mL. What is the total yield. -
ANSWER-430
ug = 860 ug/mL *
0.5mL
Calculate the RNA concentration from the following:
260=0.307 (DF 1:100) - ANSWER-1228 ug/mL = 0.307 abs * 40
ug/mL * 100
DF
An RNA preparation has the following readings:
260=0.208
280=0.096
Is this RNA suitable for use? - ANSWER-Yes, 2.17 is suitable for
RNA analysis
A260/A280 = 0.208/0.096
How does PFGE separate larger fragments more efficiently than
standard electrophoresis? - ANSWER-Repeated reorientation
forces larger fragments through the gel matrix more efficiently
,If fragments are dissolved in 50% formamide will the
stringency be higher or
lower? - ANSWER-
Higher
If fragments are dissolved in a high concentration of NaCl will
the stringency be
higher or lower? - ANSWER-
Lower
Does heating a solution from 65C to 75C during hybridization
raise or lower
stringency? - ANSWER-
raises
What would the autoradiogram show if the stringency was to
high? - ANSWER-
no
bands
Calculate the Tm of the following primers:
Forward: GGAGCTTTGTTTCAACCAAG
Reverse: ATTAAATGCGGAATTGCCCA - ANSWER-Forward:
Tm=(4C x
9GC)+(2C x 11AT) = 58C
Reverse Tm=(4C x 8GC)+(2C x 12AT) = 56C
Is 47;XXY a normal karyotype? - ANSWER-No, this is XXY
syndrome
, What is the genetic
abnormality of:
47,XY,+18 - ANSWER-Trisomy 18: Edwards
Syndrome
What is the genetic abnormality of:
46,XY,del(16p14) - ANSWER-Deletion in region 1, band 4 of the
short arm of chr 16
What is the genetic abnormality of:
iso(X)(q10) - ANSWER-Isochormosome comprised of the long
arms of the X chr
What is the genetic abnormality of:
46, XX, del(22q11.2) - ANSWER-Deletion in region 1, band 1,
sub-band 2 of the long arm of chr 22 (diGeorge's syndrome)
What is the genetic:
abnormality of
45, X - ANSWER-Monosomy X, Turner's
syndrome
How are nucleotides joined together? - ANSWER-condensation
What does mRNA do? - ANSWER-carries code out of cell.
code for protein
structure.
|| 2025 /2026 Actual Complete Real Exam Questions With
verified Answers (Correct Answers) Already Graded A+ ,
Guaranteed success| Newest Exam | Just Released !!
STUDY GUIDE EXAM
Histone acetylation close to the gene will increase or
decrease expression? -
ANSWER-
increase
siRNAs complementary to the gene transcript will
increase or decrease
expression? - ANSWER-
decrease
Calculate the DNA concentration from the
following:
260=0.172 (D.F. 1:100) - ANSWER-860 ug/mL = .172 abs * 50
ug/mL * 100 DF
[DNA] = 1535 ug/mL. You have 0.5 mL. What is the total yield.
- ANSWER-
767.5 ug = 1535 ug/mL *
0.5Ml
,[DNA] = 767.5 ug/mL. You have 0.5 mL What is the total yield.
- ANSWER-
383.75 ug = 767.5 ug/mL *
0.5 mL
[DNA] = 860 ug/mL. You have 0.5 mL. What is the total yield. -
ANSWER-430
ug = 860 ug/mL *
0.5mL
Calculate the RNA concentration from the following:
260=0.307 (DF 1:100) - ANSWER-1228 ug/mL = 0.307 abs * 40
ug/mL * 100
DF
An RNA preparation has the following readings:
260=0.208
280=0.096
Is this RNA suitable for use? - ANSWER-Yes, 2.17 is suitable for
RNA analysis
A260/A280 = 0.208/0.096
How does PFGE separate larger fragments more efficiently than
standard electrophoresis? - ANSWER-Repeated reorientation
forces larger fragments through the gel matrix more efficiently
,If fragments are dissolved in 50% formamide will the
stringency be higher or
lower? - ANSWER-
Higher
If fragments are dissolved in a high concentration of NaCl will
the stringency be
higher or lower? - ANSWER-
Lower
Does heating a solution from 65C to 75C during hybridization
raise or lower
stringency? - ANSWER-
raises
What would the autoradiogram show if the stringency was to
high? - ANSWER-
no
bands
Calculate the Tm of the following primers:
Forward: GGAGCTTTGTTTCAACCAAG
Reverse: ATTAAATGCGGAATTGCCCA - ANSWER-Forward:
Tm=(4C x
9GC)+(2C x 11AT) = 58C
Reverse Tm=(4C x 8GC)+(2C x 12AT) = 56C
Is 47;XXY a normal karyotype? - ANSWER-No, this is XXY
syndrome
, What is the genetic
abnormality of:
47,XY,+18 - ANSWER-Trisomy 18: Edwards
Syndrome
What is the genetic abnormality of:
46,XY,del(16p14) - ANSWER-Deletion in region 1, band 4 of the
short arm of chr 16
What is the genetic abnormality of:
iso(X)(q10) - ANSWER-Isochormosome comprised of the long
arms of the X chr
What is the genetic abnormality of:
46, XX, del(22q11.2) - ANSWER-Deletion in region 1, band 1,
sub-band 2 of the long arm of chr 22 (diGeorge's syndrome)
What is the genetic:
abnormality of
45, X - ANSWER-Monosomy X, Turner's
syndrome
How are nucleotides joined together? - ANSWER-condensation
What does mRNA do? - ANSWER-carries code out of cell.
code for protein
structure.
