A gene regulatory sequence which function as the binding site
for basal transcription factors to recruit RNA polymerase 2 and
initiate transcription is called? Correct Answers Promoter
A subunit within the general transcription factor ? binds to the
Tata box during transcriptional activation Correct Answers
TFIID
According to the reading what particular feature of molecular
hybridization makes it a powerful tool in molecular biology?
Correct Answers Highly specific
An activator functions by binding what element to increase
transcription in a gene specific manner Correct Answers
activators bind enhancers
an element that is a regulatory DNA sequence that binds
transcription factors to increase transcriptional activation energy
in gene specific manner Correct Answers Enhancer
Antibody generated against mouse IgG constant domain and
produced in rabbits is referred to as? Correct Answers Rabbit
anti-mouse
binds the pre-initiation complex Correct Answers TFIIH
chemical crosslinkers, give example Correct Answers Arrange
molecules in fixed close proximity.
Fromaldehyde
, Chromatin Immunoprecipitation (ChIP) Correct Answers can
determine if proteins can bind to a particular region of DNA in
the chromatin of living cells
Chromatin Immunoprecipitation (ChIP) is another technique
commonly used to study gene regulation transcription factors -
DNA interactions in (vitro/vivo). Correct Answers VIVO
clonal expansion Correct Answers the rapid multiplication of B
or T cell clones after activation by an antigen
Deleting which segment of the eucaryotic promoter would lead
to the most significant impact on transcription? Correct Answers
The TATA box
(20-30 bases upstream)
Enhancers can be found where relative to the transcriptional
start site? Correct Answers Upstream or downstream
Enzymes which function by adding a functional group to
specific histone protein amino acid residues, resulting in more
open loosely packed chromatin are Correct Answers Histone
acetyl-transferases
Function of 7-methylguanosine cap Correct Answers
Immediately caps the RNA which exits the channel of RNA Pol
2