ASCP Molecular Biology Certification Exam fully
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Terms in this set (1434)
One carbon ring
Pyrimidine
Cytosine, Thymine, Uracil
What is the function of Carries genetic info out of nucleus
mRNA? Transcript translated to protein
What is the function of Carries aa to ribosome
tRNA? Anticodon pairs with codon on mRNA strand
part of ribosome structure
What is the function of
most abundant RNA
rRNA?
coordinated coupling of tRNA to mRNA codons
Product of pathway is noncompetitive inhibitor
Feedback inhibition Binds to allosteric site to slow down rxn b/c too much
product
Restriction enzymes
Cleaves phoshpodiester bonds w/i poly-nt chain
Endonucleases (Prok)
Recognition site is palindromic sequence
Types I-V
ORI sites nt sequence where replication is initiated
, Induces ss breaks
Remove DNA supercoils during TXN and DNA
Topoisomerase I replication; for strand breakage during recombination;
for chr condensation; and to disentangle intertwined
DNA during mitosis
cuts both strands of one DNA double helix, passes
topoisomerase II another unbroken DNA helix through it, and then
reanneals the cut strands
Unwinds supercoiling caused by unwinding at the rep
Gyrase (topoisomerase II)
fork by introducing DSBs
Helicase Breaks H-bonds of double helix at the replication fork
single-strand DNA binding Binds ssDNA and prevents it from re-annealing during
proteins (SSBPs) TXN, replication, repair, and recombination
Repeat sequence (TTAGGG) at the ends of chr,
Telomeres
protect chr from degradation
DNA dependent RNApol
RNA polymerase Transcribes DNA template to RNA (3'-->5'; anti-
parallel)
Complex of snRNPs
Splicesomes Removes introns from pre-mRNA and splices exons
together
Short regions of DNA that bind proteins (TXN factors)
Enhancers
that enhance TXN of a gene
enzyme that transcribes RNA to cDNA (lacks introns)
Reverse transcriptase
RNA --> RNA:DNA --> cDNA (dsDNA)
sections of DNA that begin with start codons and end
with stop codons
Open Reading Frame
DNA: 5' --> 3'
(ORF)
transcription: 3' --> 5' DNA --> RNA (promoter)
translation: 5' --> 3' mRNA
Measures amount of light absorbed
Spectrophotometer
Quantitative measurement of [DNA/RNA]
solved & updated (latest version verified for
accuracy)| Latest!!
Save
Terms in this set (1434)
One carbon ring
Pyrimidine
Cytosine, Thymine, Uracil
What is the function of Carries genetic info out of nucleus
mRNA? Transcript translated to protein
What is the function of Carries aa to ribosome
tRNA? Anticodon pairs with codon on mRNA strand
part of ribosome structure
What is the function of
most abundant RNA
rRNA?
coordinated coupling of tRNA to mRNA codons
Product of pathway is noncompetitive inhibitor
Feedback inhibition Binds to allosteric site to slow down rxn b/c too much
product
Restriction enzymes
Cleaves phoshpodiester bonds w/i poly-nt chain
Endonucleases (Prok)
Recognition site is palindromic sequence
Types I-V
ORI sites nt sequence where replication is initiated
, Induces ss breaks
Remove DNA supercoils during TXN and DNA
Topoisomerase I replication; for strand breakage during recombination;
for chr condensation; and to disentangle intertwined
DNA during mitosis
cuts both strands of one DNA double helix, passes
topoisomerase II another unbroken DNA helix through it, and then
reanneals the cut strands
Unwinds supercoiling caused by unwinding at the rep
Gyrase (topoisomerase II)
fork by introducing DSBs
Helicase Breaks H-bonds of double helix at the replication fork
single-strand DNA binding Binds ssDNA and prevents it from re-annealing during
proteins (SSBPs) TXN, replication, repair, and recombination
Repeat sequence (TTAGGG) at the ends of chr,
Telomeres
protect chr from degradation
DNA dependent RNApol
RNA polymerase Transcribes DNA template to RNA (3'-->5'; anti-
parallel)
Complex of snRNPs
Splicesomes Removes introns from pre-mRNA and splices exons
together
Short regions of DNA that bind proteins (TXN factors)
Enhancers
that enhance TXN of a gene
enzyme that transcribes RNA to cDNA (lacks introns)
Reverse transcriptase
RNA --> RNA:DNA --> cDNA (dsDNA)
sections of DNA that begin with start codons and end
with stop codons
Open Reading Frame
DNA: 5' --> 3'
(ORF)
transcription: 3' --> 5' DNA --> RNA (promoter)
translation: 5' --> 3' mRNA
Measures amount of light absorbed
Spectrophotometer
Quantitative measurement of [DNA/RNA]
