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CTBS Exam Practice Test (75+ Questions) – Sterile Technique, Ischemic Time, Anatomy, Recovery Protocols | 2025/2026

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This high-yield exam prep document contains over 75 multiple-choice and definition-style questions with verified answers aligned with the Certified Tissue Banking Specialist (CTBS) Exam for the 2025/2026 cycle. It thoroughly covers critical topics and current AATB and FDA standards related to tissue recovery, sterility, anatomy, and transplant safety, making it an essential study tool for certification candidates. Core topics include: Sterile Technique & Contamination Control: Surgical scrub procedure, gowning, gloving, zoning protocols, and microbial threats such as Staphylococcus, Streptococcus, and Clostridium. Recovery Environment & Donor Safety: Site suitability criteria, culture timing, and physical positioning for various tissue types. Anatomy for Tissue Recovery: Includes musculoskeletal (e.g., fascia lata, tibialis, gracilis), cardiovascular, and skin graft procedures with anatomical landmarks, tendon insertions, and correct order of recovery. Skin & Graft Types: Detailed comparison of split thickness and full thickness grafts, autografts vs. allografts, meshed grafts, and dermal layer involvement. Ischemic Time Calculations: Warm and cold ischemic time for cardiac, vascular, and saphenous vein tissues, with strict compliance-based limits and temperature timelines. Cardiac & Vascular Tissue Protocols: Key perfusion times, disinfection requirements, recovery site conditions, and tissue labeling standards. This material is ideal for: CTBS Exam Candidates (AATB) Tissue Recovery Technicians Surgical Technologists Anatomy & Physiology Students Pathology Assistants and Biomedical Science Majors Public Health and Regulatory Compliance Officers in transplantation Whether you're actively preparing for the CTBS certification or reviewing best practices in surgical recovery and tissue banking, this practice test provides clear, structured, and certification-relevant content. Keywords: CTBS, sterile technique, ischemic time, tissue banking, graft recovery, musculoskeletal anatomy, surgical scrub, cardiovascular tissue, FDA, AATB, tendon landmarks, split thickness skin, donor eligibility, contamination control, donor anatomy, recovery protocol

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CTBS 2025 EXAM 2025/2026 EXAM
QUESTIONS AND ANSWERS | 100%
PASS



Sterile technique is required by what standards - 🧠 ANSWER ✔✔AATB

AND FDA


stewardship of the gift (MMA) - 🧠 ANSWER ✔✔1. maximize quality of donor


2. minimize infection potential

3. avoid unnecessary tissue discard


can you wear street clothes under scrubs? - 🧠 ANSWER ✔✔no


Criteria for determining suitability of recovery site - 🧠 ANSWER ✔✔1.

adequate floor and tabletop space

,2. zoning and recovery sequencing ( contamination control method to make

sure each zone is recovered and wrapped separately from other to make

sure there is no x-contam of bio burden from one zone to another.

3. lighting

4. plumbing and draining

4. controlled closed air flow (no access to outside, no windows, ac, fan etc)

5. all vents appear clean

6. no vented airflow directed onto sterile field

T/F: Handwashing can be substituted for use of approved waterless scrub

option - 🧠 ANSWER ✔✔true


when do cultures to monitor bio burden occur - 🧠 ANSWER ✔✔post

recovery

- before processing begins

post processing

- before final packaging

T/F: freezer quarantine kills all bio burden present during recovery - 🧠

ANSWER ✔✔false

, what bio burden can survive the freezer quarantine (MYEC) - 🧠 ANSWER

✔✔mold, yeast, e. coli and clostridium


5 major sources of contamination - 🧠 ANSWER ✔✔1. air


2. hair - cover our hair and shave donor

3. skin - cover our skin and leave only recover areas of donor are exposed

4. orifices - cover mouth/nose

5. fomites - dust particles.


3 things surgical scrub removes - 🧠 ANSWER ✔✔1. dirt from hands and

arms

2/ oils from skin

3. bacteria from skin, under fingernails and cuticles.


initial kill - 🧠 ANSWER ✔✔kills bacteria on contact


residual kill - 🧠 ANSWER ✔✔soap on the skin keeps killing bacteria


appropriate detergent for surgical scrub - 🧠 ANSWER ✔✔CHG, iodine,

PCMX


steps for hand scrub - 🧠 ANSWER ✔✔1. wash hands thoroughly


COPYRIGHT©NINJANERD 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER: 619652435. TERMS OF USE. PRIVACY
STATEMENT. ALL RIGHTS RESERVED
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