CG(ASCP) EXAM REVIEW QUESTIONS
AND ANSWERS WITH COMPLETE
SOLUTIONS 100% CORRECT!!!
Pepsin pretreatment
✓ A method used to enhance the intensity of FISH probes.
2x SSC
✓ A buffer solution used to improve the intensity of FISH probes.
Wilm's tumor
✓ A type of cancer associated with abnormalities in chromosome 11.
Ewing sarcoma
✓ A type of cancer associated with the t(11;22) translocation.
Retinoblastoma
✓ A type of eye cancer associated with abnormalities in chromosome 13.
Down Syndrome
✓ A genetic disorder characterized by low levels of AFP and estriol, and high
levels of HCG.
Trisomy 18
✓ A genetic disorder characterized by low levels of AFP, estriol, and HCG.
, Slides kept for 3 years -ANSWER✔✔A guideline from CAP for the retention of
slides.
Prenatal -ANSWER✔✔Referring to the period of time before birth, usually up to 3
months.
Quality assurance -ANSWER✔✔A process to ensure the quality of laboratory
results, different from quality control.
Gentamycin -ANSWER✔✔The least toxic antibacterial agent to cells.
Sodium heparin -ANSWER✔✔A solution used to transport amniotic fluid at 20C.
75% confluent -ANSWER✔✔The optimal cell culture density for harvest.
DIRECT harvest -ANSWER✔✔A method to isolate cytotrophoblast for amino
culture.
No amniocentesis -ANSWER✔✔Recommended for individuals with a family
history of autosomal recessive disorders.
Antibacterial agents -ANSWER✔✔Not necessary for long-term cultures.
Ethidium bromide -ANSWER✔✔Not used as a mitotic arrestant.
pH -ANSWER✔✔Does not increase the number of chromosome breaks.
AND ANSWERS WITH COMPLETE
SOLUTIONS 100% CORRECT!!!
Pepsin pretreatment
✓ A method used to enhance the intensity of FISH probes.
2x SSC
✓ A buffer solution used to improve the intensity of FISH probes.
Wilm's tumor
✓ A type of cancer associated with abnormalities in chromosome 11.
Ewing sarcoma
✓ A type of cancer associated with the t(11;22) translocation.
Retinoblastoma
✓ A type of eye cancer associated with abnormalities in chromosome 13.
Down Syndrome
✓ A genetic disorder characterized by low levels of AFP and estriol, and high
levels of HCG.
Trisomy 18
✓ A genetic disorder characterized by low levels of AFP, estriol, and HCG.
, Slides kept for 3 years -ANSWER✔✔A guideline from CAP for the retention of
slides.
Prenatal -ANSWER✔✔Referring to the period of time before birth, usually up to 3
months.
Quality assurance -ANSWER✔✔A process to ensure the quality of laboratory
results, different from quality control.
Gentamycin -ANSWER✔✔The least toxic antibacterial agent to cells.
Sodium heparin -ANSWER✔✔A solution used to transport amniotic fluid at 20C.
75% confluent -ANSWER✔✔The optimal cell culture density for harvest.
DIRECT harvest -ANSWER✔✔A method to isolate cytotrophoblast for amino
culture.
No amniocentesis -ANSWER✔✔Recommended for individuals with a family
history of autosomal recessive disorders.
Antibacterial agents -ANSWER✔✔Not necessary for long-term cultures.
Ethidium bromide -ANSWER✔✔Not used as a mitotic arrestant.
pH -ANSWER✔✔Does not increase the number of chromosome breaks.
