ASCP CG CERTIFICATION PRACTICE
QUESTIONS AND ANSWERSWITH
COMPLETE SOLUTIONS 100%
CORRECT RATED A+
What is the best agent to use against mycoplasma?
✓ BM cyclin – A combination of two antibiotics (tiamulin and minocycline) that
effectively targets mycoplasma growth.
For successful harvest, each new batch of hypotonic solution should be
evaluated by:
✓ Comparing the new lot to a lot already in use.
Collagenase, pronase, and trypsin are:
✓ Proteolytic enzymes – Break down tissues into single-cell suspensions.
When harvesting peripheral blood cultures, aseptic technique no longer
matters following incubation with:
✓ Colcemid – A mitotic spindle poison that halts cells at metaphase.
Samples that arrive at the cytogenetics lab should first be:
✓ Logged into the system.
Phytohemagglutinin (PHA) stimulates which type of cells into division?
✓ T-cell lymphocytes.
To avoid culture failure, monitoring reagents is crucial. When logging a
culture failure, what is the MOST important to document?
✓ Expiration dates of reagents used.
,Which type of microscope is used when cleaning a CVS (chorionic villus)
sample?
✓ Dissection microscope.
Which cell type is most problematic if found in a CVS (chorionic villus)
sample?
✓ Decidua (maternal tissue).
When looking at a slide under a phase contrast scope and a lot of cytoplasm is still
seen around the mets, what is the most likely cause? -ANSWER✔✔innappropriate
slide drying time
What will help prevent microbial contamination and cross contamination of
cultures? -ANSWER✔✔Asceptic technique
When there are not enough mets to complete the study of a patient/case, what
needs to be done next? -ANSWER✔✔prepare additional slides from any
remaining sample
When looking at a dropped slide under a phase scope and mets are very spread out
and sparse, what could help fix the issue? -ANSWER✔✔Increading the drying
time by using warm slides
What is true of an inverted microscope? -ANSWER✔✔the objectives are beneath
the stage
High-resolution banding is indicated for a possible diagnosis of: -
ANSWER✔✔DiGenorge syndrome -- microdeletion of 22q11, also called
velocardiofacial syndrome
, "velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent), tubular
nose, wide spread eyes, etc
When verifying if a specimen is appropriate for cytogenetic analysis, what is the
MOST important data to accompany the specimen? -ANSWER✔✔Reason for
referral
Which type of specimen should be requested for a cytogenetic analysis for turner
syndrome? -ANSWER✔✔Heparinized peripheral blood stored at room
temperature
(NOT FROZEN)
A blood sample can be used to study the cytogenetics of a malignancy only if: -
ANSWER✔✔Dividing leukemic cells are present in circulation
All of the cultures in a CO2 incubator failed, what aspects of the incubator are
important to monitor to assess the source of the problem? -
ANSWER✔✔Temperature,
CO2 level, and
Humidity level
The most important factor affecting the quality of chromosomes viewed under a
phase contrast scope is: -ANSWER✔✔Slide drying after the cells have been
dropped
QUESTIONS AND ANSWERSWITH
COMPLETE SOLUTIONS 100%
CORRECT RATED A+
What is the best agent to use against mycoplasma?
✓ BM cyclin – A combination of two antibiotics (tiamulin and minocycline) that
effectively targets mycoplasma growth.
For successful harvest, each new batch of hypotonic solution should be
evaluated by:
✓ Comparing the new lot to a lot already in use.
Collagenase, pronase, and trypsin are:
✓ Proteolytic enzymes – Break down tissues into single-cell suspensions.
When harvesting peripheral blood cultures, aseptic technique no longer
matters following incubation with:
✓ Colcemid – A mitotic spindle poison that halts cells at metaphase.
Samples that arrive at the cytogenetics lab should first be:
✓ Logged into the system.
Phytohemagglutinin (PHA) stimulates which type of cells into division?
✓ T-cell lymphocytes.
To avoid culture failure, monitoring reagents is crucial. When logging a
culture failure, what is the MOST important to document?
✓ Expiration dates of reagents used.
,Which type of microscope is used when cleaning a CVS (chorionic villus)
sample?
✓ Dissection microscope.
Which cell type is most problematic if found in a CVS (chorionic villus)
sample?
✓ Decidua (maternal tissue).
When looking at a slide under a phase contrast scope and a lot of cytoplasm is still
seen around the mets, what is the most likely cause? -ANSWER✔✔innappropriate
slide drying time
What will help prevent microbial contamination and cross contamination of
cultures? -ANSWER✔✔Asceptic technique
When there are not enough mets to complete the study of a patient/case, what
needs to be done next? -ANSWER✔✔prepare additional slides from any
remaining sample
When looking at a dropped slide under a phase scope and mets are very spread out
and sparse, what could help fix the issue? -ANSWER✔✔Increading the drying
time by using warm slides
What is true of an inverted microscope? -ANSWER✔✔the objectives are beneath
the stage
High-resolution banding is indicated for a possible diagnosis of: -
ANSWER✔✔DiGenorge syndrome -- microdeletion of 22q11, also called
velocardiofacial syndrome
, "velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent), tubular
nose, wide spread eyes, etc
When verifying if a specimen is appropriate for cytogenetic analysis, what is the
MOST important data to accompany the specimen? -ANSWER✔✔Reason for
referral
Which type of specimen should be requested for a cytogenetic analysis for turner
syndrome? -ANSWER✔✔Heparinized peripheral blood stored at room
temperature
(NOT FROZEN)
A blood sample can be used to study the cytogenetics of a malignancy only if: -
ANSWER✔✔Dividing leukemic cells are present in circulation
All of the cultures in a CO2 incubator failed, what aspects of the incubator are
important to monitor to assess the source of the problem? -
ANSWER✔✔Temperature,
CO2 level, and
Humidity level
The most important factor affecting the quality of chromosomes viewed under a
phase contrast scope is: -ANSWER✔✔Slide drying after the cells have been
dropped
