CG(ASCP) EXAM QUESTIONS AND
ANSWERS WITH COMPLETE
SOLUTIONNS 100% CORRECT RATED
A+ NEWLY UPDATED 2025
The most important reason why biological safety cabinets are used instead of
horizontal laminar flow hoods in the handling of human tissues is:
✓ To protect the technologist who is processing the specimens.
In which of the following situations would one suspect bacterial contamination?
✓ Culture media is turbid in appearance.
Which of the following chemicals is used for cryopreservation?
✓ Dimethyl sulfoxide (DMSO).
Which of the following is a buffer used in cultures?
✓ HEPES.
In order for fragile X to be expressed in affected individuals, lymphocytes must be
grown in what type of media?
✓ Folic acid deficient.
The following enzymes can be used to dissociate Chronic Villus samples (CVS),
except for:
✓ RNAse.
Which of the following is not a necessary ingredient added to modified medium?
✓ Antibiotics.
,All of the following are factors which affect cryopreservation, except:
(Answer needs additional context to complete.)
A fast-growing culture shows signs of cell death. What is a possible reason for
this?
✓ Cell confluency: too many or too few cells.
Collagenase, pronase, and trypsin are used to disperse tissue into a single-cell
suspension. These substances are:
✓ Proteolytic enzymes.
When harvesting peripheral blood cultures, aseptic technique no longer matters
following incubation with:
✓ Colcemid.
Which of the following culture processes would be most useful for identifying
patients with Fanconi anemia?
✓ Mitomycin-C stress test of peripheral blood.
To avoid potential culture failure, it is important to monitor the reagents used.
Recording which of the following information will be most important when
documenting culture failure?
✓ Expiration date.
Which of the following is not a necessary ingredient which is added to modified
medium?
antibiotics
,All of the following are factors which affect the cryopreservation except
A fast growing culture shows signs of cell death. What is a possible reason for
this?
cell confluency: too many or too few cells
collagenase, pronase, and trypsin are used to disperse tissue into single-cell
suspension. These substances are:
proteolytic enzymes
When harvesting peripheral blood cultures, asceptic technique no longer matters
following incubation with:
colcemid
Which of the following culture processes would be most useful for identifying
patients w/ fanconi anemia?
mitomycin-C stress test of peripheral blood
To avoid potential culture failure, it is important to monitor the reagents used.
Recording which of the following information will be most important when
documenting culture failure?
, expiration date
The morning after being set-up, a single dish of amniotic fluid culture turns cloudy
and appears contaminated. None of the other cultures set up that day appear
contaminated. Which of the following should be done first?
harvest the culture as soon as possible
If an amniotic fluid culture from the same sample grows well in a different
incubator, the probable cause of the culture failure is
incubator failure
Positive staining w/ Hoechst 33258 reveals micronuclei. Which of the following
agents caused the culture contamination?
mycoplasma
Which of the following statements is true about trypsin>
is is useful in removing cells from culture flasks
Which of the following is the best tissue to use for both a good success rate and
abnormality detection in spontaneous abortion specimen
chorionic villi
ANSWERS WITH COMPLETE
SOLUTIONNS 100% CORRECT RATED
A+ NEWLY UPDATED 2025
The most important reason why biological safety cabinets are used instead of
horizontal laminar flow hoods in the handling of human tissues is:
✓ To protect the technologist who is processing the specimens.
In which of the following situations would one suspect bacterial contamination?
✓ Culture media is turbid in appearance.
Which of the following chemicals is used for cryopreservation?
✓ Dimethyl sulfoxide (DMSO).
Which of the following is a buffer used in cultures?
✓ HEPES.
In order for fragile X to be expressed in affected individuals, lymphocytes must be
grown in what type of media?
✓ Folic acid deficient.
The following enzymes can be used to dissociate Chronic Villus samples (CVS),
except for:
✓ RNAse.
Which of the following is not a necessary ingredient added to modified medium?
✓ Antibiotics.
,All of the following are factors which affect cryopreservation, except:
(Answer needs additional context to complete.)
A fast-growing culture shows signs of cell death. What is a possible reason for
this?
✓ Cell confluency: too many or too few cells.
Collagenase, pronase, and trypsin are used to disperse tissue into a single-cell
suspension. These substances are:
✓ Proteolytic enzymes.
When harvesting peripheral blood cultures, aseptic technique no longer matters
following incubation with:
✓ Colcemid.
Which of the following culture processes would be most useful for identifying
patients with Fanconi anemia?
✓ Mitomycin-C stress test of peripheral blood.
To avoid potential culture failure, it is important to monitor the reagents used.
Recording which of the following information will be most important when
documenting culture failure?
✓ Expiration date.
Which of the following is not a necessary ingredient which is added to modified
medium?
antibiotics
,All of the following are factors which affect the cryopreservation except
A fast growing culture shows signs of cell death. What is a possible reason for
this?
cell confluency: too many or too few cells
collagenase, pronase, and trypsin are used to disperse tissue into single-cell
suspension. These substances are:
proteolytic enzymes
When harvesting peripheral blood cultures, asceptic technique no longer matters
following incubation with:
colcemid
Which of the following culture processes would be most useful for identifying
patients w/ fanconi anemia?
mitomycin-C stress test of peripheral blood
To avoid potential culture failure, it is important to monitor the reagents used.
Recording which of the following information will be most important when
documenting culture failure?
, expiration date
The morning after being set-up, a single dish of amniotic fluid culture turns cloudy
and appears contaminated. None of the other cultures set up that day appear
contaminated. Which of the following should be done first?
harvest the culture as soon as possible
If an amniotic fluid culture from the same sample grows well in a different
incubator, the probable cause of the culture failure is
incubator failure
Positive staining w/ Hoechst 33258 reveals micronuclei. Which of the following
agents caused the culture contamination?
mycoplasma
Which of the following statements is true about trypsin>
is is useful in removing cells from culture flasks
Which of the following is the best tissue to use for both a good success rate and
abnormality detection in spontaneous abortion specimen
chorionic villi
