ASCP CG EXAM (MOLECULAR CG
TESTING) QUESTIONS AND ANSWERS
WITH CORRECT RATED A+
Which counterstain dye should be used to counterstain cells with Spectrum
Green/Spectrum Red dual color signals?
✓ DAPI.
DAPI is a _______ counterstain.
✓ Blue.
The process of FISH is used to:
✓ Visualize a specific sequence of DNA with a fluorochrome.
Which factor is critical to the success of a FISH procedure?
✓ Temperature of the denature solution.
Formamide is used in the FISH procedure for which of the following
purposes?
✓ To lower the DNA melting point.
What is the purpose of the post-hybridization wash?
✓ To remove unbound probe from the slide.
What is true about slide preparation for FISH?
✓ Slides should be kept at room temperature conditions.
When analyzing a sample stained with a FISH probe, the technologist notices
a high amount of nonspecific cross-hybridization. What is most likely the
, cause?
✓ The stringency was too low.
When using the Applied Imaging system to capture the FISH image, which
program should be chosen?
✓ Probe.
Which of the following statements is true about directly labeled probes? -
ANSWER✔✔Exposure to light should be avoided.
All of the following are advantages of array-CGH except? -ANSWER✔✔The use
of metaphase chromosomes.
Which of the following regarding test resolution is correct? -ANSWER✔✔BAC
array has lower resolution than oligo array.
Which of the following are the QC measurements for array-CGH? -
ANSWER✔✔Triplicate clones used, dye swap, and equal amount
sample/reference DNA.
True/False: Array-based CGH is a molecular technique that detects balanced
genomic rearrangements (translocations and insertions ) by comparing test DNA to
normal reference DNA. -ANSWER✔✔False.
Which is not an indication of aCGH testing? -ANSWER✔✔A mother carries an
inverted 9 found by karyotyping analysis.
TESTING) QUESTIONS AND ANSWERS
WITH CORRECT RATED A+
Which counterstain dye should be used to counterstain cells with Spectrum
Green/Spectrum Red dual color signals?
✓ DAPI.
DAPI is a _______ counterstain.
✓ Blue.
The process of FISH is used to:
✓ Visualize a specific sequence of DNA with a fluorochrome.
Which factor is critical to the success of a FISH procedure?
✓ Temperature of the denature solution.
Formamide is used in the FISH procedure for which of the following
purposes?
✓ To lower the DNA melting point.
What is the purpose of the post-hybridization wash?
✓ To remove unbound probe from the slide.
What is true about slide preparation for FISH?
✓ Slides should be kept at room temperature conditions.
When analyzing a sample stained with a FISH probe, the technologist notices
a high amount of nonspecific cross-hybridization. What is most likely the
, cause?
✓ The stringency was too low.
When using the Applied Imaging system to capture the FISH image, which
program should be chosen?
✓ Probe.
Which of the following statements is true about directly labeled probes? -
ANSWER✔✔Exposure to light should be avoided.
All of the following are advantages of array-CGH except? -ANSWER✔✔The use
of metaphase chromosomes.
Which of the following regarding test resolution is correct? -ANSWER✔✔BAC
array has lower resolution than oligo array.
Which of the following are the QC measurements for array-CGH? -
ANSWER✔✔Triplicate clones used, dye swap, and equal amount
sample/reference DNA.
True/False: Array-based CGH is a molecular technique that detects balanced
genomic rearrangements (translocations and insertions ) by comparing test DNA to
normal reference DNA. -ANSWER✔✔False.
Which is not an indication of aCGH testing? -ANSWER✔✔A mother carries an
inverted 9 found by karyotyping analysis.
