AMT MLS EXAM UPDATED QUESTIONS
AND CORRECT ANSWERS.
Which immunoassay methodology is the emission of light by molecules in an excited state with
a limited amount of heat as the result of a chemical reaction?
Question
a.Chemiluminescence
b.Immunochromatography
c.Photometry
d.Spectrophotometry - ANS a. Chemiluminescence
Chemiluminescence is the emission of light by molecules in an excited state with a limited
amount of heat (luminescence) as the result of a chemical reaction. Chemiluminescent assays
are ultrasensitive and are widely used in automated immunoassays and DNA probe assay
systems. Chemiluminescence has excellent sensitivity and dynamic range. Chemiluminescent
labels can be attached to an antigen or an antibody. Most chemiluminescent reagents and
conjugates are stable and relatively nontoxic.
What characteristics/functions do calibrators have?
Question
a.Contains a known amount of analyte being tested
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,b.Monitors the quality of reagents
c.Monitors the quality of the sample
d.Prevents equipment failure - ANS a. Contains a known amount of analyte being tested
Calibrators are used to establish a correlation between the measurement response and the
concentration or amount of the substance that is being measured by the test procedure.
Absorbance can be calculated easily from percent transmission using which calculation?
a.2 - log10 %T
b.2 + log10 %T
c.log10 %T - 2
d.log10 %T + 2 - ANS a. 2 - log10 %T
Absorbance can be calculated easily from percent transmission using 2 - log10 %T. If all the light
passes through a solution without any absorption, absorbance is zero and the %T is 100%. If all
the light is absorbed, %T is zero, and absorption is infinite.
What is a substance or constituent in which the laboratory conducts testing?
a.A solution
b.An additive
c.An analyte
d.An anticoagulant - ANS c. An analyte
In the testing process, analytes or constituents are measured.
2 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
,What does a common application of the polarized light microscope in the clinical laboratory do?
Determine FANAs with fluorescence
b.Determine neutrophilic from lymphocytic leukemia
c.Examine histochemical reactions
d.Study crystals in urine and in cells - ANS d. study crystals in urine and cells
What is the chelation of calcium in the EDTA tube?
a.Maintains a constant temperature for components
b.Prevents coagulation by inhibiting coagulation
c.Prevents the separation of blood components
d.Stops the lysing of the red blood cells - ANS b. Prevents coagulation by inhibiting
coagulation
CLSI recommends the salts of the chelating (calcium binding) agent EDTA as the anticoagulant of
choice for blood cell counting and sizing.
How must a laboratory operate to handle TB sputum and TM materials?
a.Biosafety level of 2+ or 3
b.Biosafety level 4
c.Location where air exchange is continuous
d.Location where fume hood exhausts are monitored daily - ANS a. Biosafety level of 2+ or 3
3 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
, BSL-3 builds upon the requirements of BSL-2. Organisms that can cause serious or potentially
lethal disease through respiratory transmission are typically worked with in a BSL-3 laboratory.
Example: Mycobacterium tuberculosis.
What are Class A fire extinguishers used for?
a.Electric equipment
b.Flammable liquids
c.Ordinary combustibles
d.Powdered metal (combustible) material - ANS Ordinary combustibles.
Class A fire extinguishers are classified as ordinary combustible. Additionally, Class A fire
extinguishers have a numerical rating based on UL-conducted tests and designed to determine
the extinguishing potential for each size and type of extinguisher
Which part of the microscope directs and focuses the beam of light from the bulb onto the
material under examination?
a.Aperture
b.C- mount
c.Condenser
d.Diaphragm - ANS Condenser
The condenser is the substage lens of the microscope that focuses light on the specimen slide
How many milliliters can a 3% solution make if 6 grams of solute are available?
a.100 mL
4 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
AND CORRECT ANSWERS.
Which immunoassay methodology is the emission of light by molecules in an excited state with
a limited amount of heat as the result of a chemical reaction?
Question
a.Chemiluminescence
b.Immunochromatography
c.Photometry
d.Spectrophotometry - ANS a. Chemiluminescence
Chemiluminescence is the emission of light by molecules in an excited state with a limited
amount of heat (luminescence) as the result of a chemical reaction. Chemiluminescent assays
are ultrasensitive and are widely used in automated immunoassays and DNA probe assay
systems. Chemiluminescence has excellent sensitivity and dynamic range. Chemiluminescent
labels can be attached to an antigen or an antibody. Most chemiluminescent reagents and
conjugates are stable and relatively nontoxic.
What characteristics/functions do calibrators have?
Question
a.Contains a known amount of analyte being tested
1 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
,b.Monitors the quality of reagents
c.Monitors the quality of the sample
d.Prevents equipment failure - ANS a. Contains a known amount of analyte being tested
Calibrators are used to establish a correlation between the measurement response and the
concentration or amount of the substance that is being measured by the test procedure.
Absorbance can be calculated easily from percent transmission using which calculation?
a.2 - log10 %T
b.2 + log10 %T
c.log10 %T - 2
d.log10 %T + 2 - ANS a. 2 - log10 %T
Absorbance can be calculated easily from percent transmission using 2 - log10 %T. If all the light
passes through a solution without any absorption, absorbance is zero and the %T is 100%. If all
the light is absorbed, %T is zero, and absorption is infinite.
What is a substance or constituent in which the laboratory conducts testing?
a.A solution
b.An additive
c.An analyte
d.An anticoagulant - ANS c. An analyte
In the testing process, analytes or constituents are measured.
2 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
,What does a common application of the polarized light microscope in the clinical laboratory do?
Determine FANAs with fluorescence
b.Determine neutrophilic from lymphocytic leukemia
c.Examine histochemical reactions
d.Study crystals in urine and in cells - ANS d. study crystals in urine and cells
What is the chelation of calcium in the EDTA tube?
a.Maintains a constant temperature for components
b.Prevents coagulation by inhibiting coagulation
c.Prevents the separation of blood components
d.Stops the lysing of the red blood cells - ANS b. Prevents coagulation by inhibiting
coagulation
CLSI recommends the salts of the chelating (calcium binding) agent EDTA as the anticoagulant of
choice for blood cell counting and sizing.
How must a laboratory operate to handle TB sputum and TM materials?
a.Biosafety level of 2+ or 3
b.Biosafety level 4
c.Location where air exchange is continuous
d.Location where fume hood exhausts are monitored daily - ANS a. Biosafety level of 2+ or 3
3 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
, BSL-3 builds upon the requirements of BSL-2. Organisms that can cause serious or potentially
lethal disease through respiratory transmission are typically worked with in a BSL-3 laboratory.
Example: Mycobacterium tuberculosis.
What are Class A fire extinguishers used for?
a.Electric equipment
b.Flammable liquids
c.Ordinary combustibles
d.Powdered metal (combustible) material - ANS Ordinary combustibles.
Class A fire extinguishers are classified as ordinary combustible. Additionally, Class A fire
extinguishers have a numerical rating based on UL-conducted tests and designed to determine
the extinguishing potential for each size and type of extinguisher
Which part of the microscope directs and focuses the beam of light from the bulb onto the
material under examination?
a.Aperture
b.C- mount
c.Condenser
d.Diaphragm - ANS Condenser
The condenser is the substage lens of the microscope that focuses light on the specimen slide
How many milliliters can a 3% solution make if 6 grams of solute are available?
a.100 mL
4 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
