Genetics Exam test quiz and
answers graded A+
Tata Box - ANS✅✅binding site for RNA polymerase II in eukaryotes
polyadenylation signal - ANS✅✅the signal to add a long string of A residues at the end of
eukaryotic mRNA
carboxy terminal domain (CTD) - ANS✅✅part of beta subunit of a eukaryotic RNA polymerase II is
essential for processing of pre-mRNA
poly(a) tail - ANS✅✅added at the 3' end of eukaryotic mRNA at the end of transcription.
Tata box - ANS✅✅small DNA segment upstream of transcription initiation site binds to TBP in
eukaryotes
A residue at branch point - ANS✅✅This residue in a eukaryotic intron of pre-mRNA binds to the
small nuclear protein complex U2.
False - ANS✅✅RNA polymerases use a sense strand as a template to synthesize RNA.
False - ANS✅✅Splicing occurs in prokaryotes.
False - ANS✅✅Capping by m7G is the last step of pre-mRNA maturation.
False - ANS✅✅Like eukaryotes, prokaryotes have three RNA polymerases.
True - ANS✅✅RISC is required for miRNA to function.
False - ANS✅✅Splicing occurs in the cytoplasm.
, False - ANS✅✅Formation of a hairpin structure is a signal for transcription termination in
eukaryotes.
True - ANS✅✅The hydroxyl group (-OH) at the 2' position of adenosine ribonucleotide at the
branch point plays a key role in splicing.
False - ANS✅✅Alternative splicing is the only way to produce several isoforms from a single gene.
True - ANS✅✅The GU-AG rule in splicing was discovered by aligning 5' and 3' junctions of exons
and introns.
DNA vs RNA - ANS✅✅DNA: double stranded
a=t, g=c
H at C2
Rna: single stranded
G=C, U=A
OH at C2
purines - ANS✅✅Adenine and Guanine (2 rings)
Pyrimidines - ANS✅✅cytosine, thymine, uracil (3 rings)
3 steps of prokaryotic transcription - ANS✅✅initiation
elongation
termination
sigma factor - ANS✅✅binding to promoter (-35 to -10 region) melts the DNA double helix
RNA polymerase holoenzyme - ANS✅✅bacterial RNA polymerase that scans the DNA for a
promoter sequence
answers graded A+
Tata Box - ANS✅✅binding site for RNA polymerase II in eukaryotes
polyadenylation signal - ANS✅✅the signal to add a long string of A residues at the end of
eukaryotic mRNA
carboxy terminal domain (CTD) - ANS✅✅part of beta subunit of a eukaryotic RNA polymerase II is
essential for processing of pre-mRNA
poly(a) tail - ANS✅✅added at the 3' end of eukaryotic mRNA at the end of transcription.
Tata box - ANS✅✅small DNA segment upstream of transcription initiation site binds to TBP in
eukaryotes
A residue at branch point - ANS✅✅This residue in a eukaryotic intron of pre-mRNA binds to the
small nuclear protein complex U2.
False - ANS✅✅RNA polymerases use a sense strand as a template to synthesize RNA.
False - ANS✅✅Splicing occurs in prokaryotes.
False - ANS✅✅Capping by m7G is the last step of pre-mRNA maturation.
False - ANS✅✅Like eukaryotes, prokaryotes have three RNA polymerases.
True - ANS✅✅RISC is required for miRNA to function.
False - ANS✅✅Splicing occurs in the cytoplasm.
, False - ANS✅✅Formation of a hairpin structure is a signal for transcription termination in
eukaryotes.
True - ANS✅✅The hydroxyl group (-OH) at the 2' position of adenosine ribonucleotide at the
branch point plays a key role in splicing.
False - ANS✅✅Alternative splicing is the only way to produce several isoforms from a single gene.
True - ANS✅✅The GU-AG rule in splicing was discovered by aligning 5' and 3' junctions of exons
and introns.
DNA vs RNA - ANS✅✅DNA: double stranded
a=t, g=c
H at C2
Rna: single stranded
G=C, U=A
OH at C2
purines - ANS✅✅Adenine and Guanine (2 rings)
Pyrimidines - ANS✅✅cytosine, thymine, uracil (3 rings)
3 steps of prokaryotic transcription - ANS✅✅initiation
elongation
termination
sigma factor - ANS✅✅binding to promoter (-35 to -10 region) melts the DNA double helix
RNA polymerase holoenzyme - ANS✅✅bacterial RNA polymerase that scans the DNA for a
promoter sequence
