1|Page
NBME CBSE BIOCHEMISTRY METABOLISM
TEST EXAM VERIFIED QUESTIONS & 100%
ACCURATE ANSWERS | COMPLETELY
UPDATED 2025–2026 EDITION A+
PERFORMANCE GUARANTEED
What glucose transporter is insulin-independent and is found in the brain and
RBCs.
GLUT-1
What glucose transporter is insulin-dependent and is found in fat tissue and
skeletal muscle?
GLUT-4
What glucose transporter is insulin independent, is bidirectional, is found in the
liver, kidney, intestine, and pancrease?
GLUT-2
What enzyme functions like hexokinase in the liver and pancreas and is NOT
inhibited by G6P but rather by F6P (overcome by high glu).
Glucokinase
,2|Page
What enzyme regulates glucokinase by taking it to the nucleus and in what
condition does it inhibit the enzyme?
glucokinase regulatory protein, low blood sugar, low insulin
What enzyme deficiency results in hyperglycemia which makes the pancreas less
sensitive to glucose and is often exacerbated in pregnancy?
glucokinase deficiency (lhe livers ability to store glucose as glycogen is impaired)
T/F If F-2,6-bisphosphate is high then gluconeogenesis is occurring.
False: If F-2,6-bisphosphate is high then glycolysis is occurring. (insulin increase
PFK2 to increase F 2,6 BP levels, glucagon decreases)
What condition is characterized by low pH, low bicarbonate, elevates anion gap
acidosis, increase lactic acid production and sepsis?
lactic acidosis (low O2, low TCA and ETC)
, 3|Page
What deficiency is an autosomal recessive disorder in a newborn and is
characterized by low lactic acid production, low citrate, extravascular hemolysis,
and splenomegaly?
Pyruvate Kinase Deficiency (low pyruvate)
How much ATP is generated in an O2 condition using the malate-aspartate shuttle
(liver, heart) versus using the glycerol-3-phosphate shuttle (muscle)?
1) malate-aspartate shuttle (liver, heart): 32 ATP
2) glycerol-3-phosphate shuttle (muscle): 30 APT
How much ATP is generated in NO O2 condition?
2 ATP
What amino acid inhibits pyruvate kinase along with ATP?
alanine
NBME CBSE BIOCHEMISTRY METABOLISM
TEST EXAM VERIFIED QUESTIONS & 100%
ACCURATE ANSWERS | COMPLETELY
UPDATED 2025–2026 EDITION A+
PERFORMANCE GUARANTEED
What glucose transporter is insulin-independent and is found in the brain and
RBCs.
GLUT-1
What glucose transporter is insulin-dependent and is found in fat tissue and
skeletal muscle?
GLUT-4
What glucose transporter is insulin independent, is bidirectional, is found in the
liver, kidney, intestine, and pancrease?
GLUT-2
What enzyme functions like hexokinase in the liver and pancreas and is NOT
inhibited by G6P but rather by F6P (overcome by high glu).
Glucokinase
,2|Page
What enzyme regulates glucokinase by taking it to the nucleus and in what
condition does it inhibit the enzyme?
glucokinase regulatory protein, low blood sugar, low insulin
What enzyme deficiency results in hyperglycemia which makes the pancreas less
sensitive to glucose and is often exacerbated in pregnancy?
glucokinase deficiency (lhe livers ability to store glucose as glycogen is impaired)
T/F If F-2,6-bisphosphate is high then gluconeogenesis is occurring.
False: If F-2,6-bisphosphate is high then glycolysis is occurring. (insulin increase
PFK2 to increase F 2,6 BP levels, glucagon decreases)
What condition is characterized by low pH, low bicarbonate, elevates anion gap
acidosis, increase lactic acid production and sepsis?
lactic acidosis (low O2, low TCA and ETC)
, 3|Page
What deficiency is an autosomal recessive disorder in a newborn and is
characterized by low lactic acid production, low citrate, extravascular hemolysis,
and splenomegaly?
Pyruvate Kinase Deficiency (low pyruvate)
How much ATP is generated in an O2 condition using the malate-aspartate shuttle
(liver, heart) versus using the glycerol-3-phosphate shuttle (muscle)?
1) malate-aspartate shuttle (liver, heart): 32 ATP
2) glycerol-3-phosphate shuttle (muscle): 30 APT
How much ATP is generated in NO O2 condition?
2 ATP
What amino acid inhibits pyruvate kinase along with ATP?
alanine
