Molecular Biology Exam test quiz
and answers graded A+
What proteins are involved with transport of proteins into the nucleus? - ANS✅✅Ran and importin
what genes are inherited only from a person's mother? - ANS✅✅cytochrome oxidase and NADH
DH
in addition to providing evidence that DNA was the genetic material, Chargaff's rules also helped
demonstrate that: - ANS✅✅C and G as well as A and T formed Hydrogen bonds
in eukaryotic chromosomes, DNA is organized into repeating units about 200 BP long because: -
ANS✅✅DNa is wrapped around nucleosomes
what is not used in a PCR reaction? - ANS✅✅dideoxynucleoside triphosphates
unlike other methods of sequencing DNA, Oxford nanopore and Pacific Biosystems - ANS✅✅can
sequence single DNA molecules
topoisomerases are required for DNA replication in cells becuase - ANS✅✅chromosomes have
supercoiled regions
difficulties arise with the replication of DNA at the telomeres of eukaryotic chromosomes because -
ANS✅✅RNA primers cannot be replaced by DNA
when bacterial genes are transcribed, what portion of RNA polymerase recognizes the promoter? -
ANS✅✅the sigma subunit
what genes are on the mitochondrial genome? - ANS✅✅cytochrome oxidase and NADH DH,
tRNA's, ATP synthase
what protein is involved in transporting RNA's and proteins out of the nucelus? - ANS✅✅Ran and
exportin
, what must happen in order to begin DNA replication? - ANS✅✅uncoil chromosomes
when eukaryotic genes are transcribed, what portion of DNA polymerase recognizes the promoter? -
ANS✅✅the TATA binding protein (TBP)
this RNA polymerase transcribes the genes for tRNA's in human cells - ANS✅✅III
this RNA polymerase transcribes the genes for rRNA's in human cells - ANS✅✅I
this RNA polymerase transcribes the genes for mRNA's in human cells - ANS✅✅II
a frame-shift mutation which adds ________ nucleotides to a gene can often produce a functional
protein - ANS✅✅a multiple of 3 (3, 6, 9 etc)
monitoring the transcription of many genes in cells using gene microchip arrays and RNA-seq
requires - ANS✅✅an initial step with viral reverse transcriptase
a western blot is most useful to - ANS✅✅measure concentration and size of one protein
the first (amino-terminal) amino acid incorporated into a bacterial polypeptide is - ANS✅✅n-
formyl methionine (AUG)
the process occurs at the A site of the ribosome - ANS✅✅binding of amino-acyl-tRNA's
the first (amino-terminal) amino acid incorporated into a eukaryotic polypeptide is -
ANS✅✅methionine
newly-synthesized polypeptides made in the cytosol enter mitochondria through - ANS✅✅TOM
and TIM
high-level transcription of the beta-galactosidase gene in E. coli, whether or not glucose is absent
(provided that lactose is present) occurs if a mutation produces - ANS✅✅a CAP protein which is
always active
and answers graded A+
What proteins are involved with transport of proteins into the nucleus? - ANS✅✅Ran and importin
what genes are inherited only from a person's mother? - ANS✅✅cytochrome oxidase and NADH
DH
in addition to providing evidence that DNA was the genetic material, Chargaff's rules also helped
demonstrate that: - ANS✅✅C and G as well as A and T formed Hydrogen bonds
in eukaryotic chromosomes, DNA is organized into repeating units about 200 BP long because: -
ANS✅✅DNa is wrapped around nucleosomes
what is not used in a PCR reaction? - ANS✅✅dideoxynucleoside triphosphates
unlike other methods of sequencing DNA, Oxford nanopore and Pacific Biosystems - ANS✅✅can
sequence single DNA molecules
topoisomerases are required for DNA replication in cells becuase - ANS✅✅chromosomes have
supercoiled regions
difficulties arise with the replication of DNA at the telomeres of eukaryotic chromosomes because -
ANS✅✅RNA primers cannot be replaced by DNA
when bacterial genes are transcribed, what portion of RNA polymerase recognizes the promoter? -
ANS✅✅the sigma subunit
what genes are on the mitochondrial genome? - ANS✅✅cytochrome oxidase and NADH DH,
tRNA's, ATP synthase
what protein is involved in transporting RNA's and proteins out of the nucelus? - ANS✅✅Ran and
exportin
, what must happen in order to begin DNA replication? - ANS✅✅uncoil chromosomes
when eukaryotic genes are transcribed, what portion of DNA polymerase recognizes the promoter? -
ANS✅✅the TATA binding protein (TBP)
this RNA polymerase transcribes the genes for tRNA's in human cells - ANS✅✅III
this RNA polymerase transcribes the genes for rRNA's in human cells - ANS✅✅I
this RNA polymerase transcribes the genes for mRNA's in human cells - ANS✅✅II
a frame-shift mutation which adds ________ nucleotides to a gene can often produce a functional
protein - ANS✅✅a multiple of 3 (3, 6, 9 etc)
monitoring the transcription of many genes in cells using gene microchip arrays and RNA-seq
requires - ANS✅✅an initial step with viral reverse transcriptase
a western blot is most useful to - ANS✅✅measure concentration and size of one protein
the first (amino-terminal) amino acid incorporated into a bacterial polypeptide is - ANS✅✅n-
formyl methionine (AUG)
the process occurs at the A site of the ribosome - ANS✅✅binding of amino-acyl-tRNA's
the first (amino-terminal) amino acid incorporated into a eukaryotic polypeptide is -
ANS✅✅methionine
newly-synthesized polypeptides made in the cytosol enter mitochondria through - ANS✅✅TOM
and TIM
high-level transcription of the beta-galactosidase gene in E. coli, whether or not glucose is absent
(provided that lactose is present) occurs if a mutation produces - ANS✅✅a CAP protein which is
always active
