Molecular Biology Exam test quiz
and answers graded A+
What are the four major macromolecules of the cell? - ANS✅✅carbohydrates, lipids, nucleic acids,
proteins
What is the function role of DNA in the cell? - ANS✅✅To store all genetic information and encode
amino acids using the genetic code
What molecular biology tool did we get from a bacterium called Thermus aquatics? - ANS✅✅Taq
polymerase
What type of enzyme do we use to "cut" DNA? - ANS✅✅restriction enzymes
What type of bonds "glue" DNA strands together? - ANS✅✅hydrogen bonds
What is the ultimate goal of PCR? - ANS✅✅to make copies of DNA
What is the purpose of DNA sequencing? - ANS✅✅to look at all of the letter in a sequence of DNA
In gel electrophoresis, why do the smaller fragments run faster? - ANS✅✅because they are able to
work their way through the gel matrix easier due to their smaller size, the larger fragments are going
to get hung up in the matrix and therefore move slower.
In gel electrophoresis, why does the positive pole need to be place at the bottom of the gel? -
ANS✅✅Because DNA is negatively charged and it will therefore make the DNA run towards the
bottom of the gel
What normal biological process does PCR most closely resemble? - ANS✅✅DNA replication
What are the three major steps in PCR? - ANS✅✅denaturation, annealing, extension
and answers graded A+
What are the four major macromolecules of the cell? - ANS✅✅carbohydrates, lipids, nucleic acids,
proteins
What is the function role of DNA in the cell? - ANS✅✅To store all genetic information and encode
amino acids using the genetic code
What molecular biology tool did we get from a bacterium called Thermus aquatics? - ANS✅✅Taq
polymerase
What type of enzyme do we use to "cut" DNA? - ANS✅✅restriction enzymes
What type of bonds "glue" DNA strands together? - ANS✅✅hydrogen bonds
What is the ultimate goal of PCR? - ANS✅✅to make copies of DNA
What is the purpose of DNA sequencing? - ANS✅✅to look at all of the letter in a sequence of DNA
In gel electrophoresis, why do the smaller fragments run faster? - ANS✅✅because they are able to
work their way through the gel matrix easier due to their smaller size, the larger fragments are going
to get hung up in the matrix and therefore move slower.
In gel electrophoresis, why does the positive pole need to be place at the bottom of the gel? -
ANS✅✅Because DNA is negatively charged and it will therefore make the DNA run towards the
bottom of the gel
What normal biological process does PCR most closely resemble? - ANS✅✅DNA replication
What are the three major steps in PCR? - ANS✅✅denaturation, annealing, extension
