Molecular Biology Exam test queries and
answers graded A+
1. In the PCR samples 2 genes of interest and 1 control.
we made, each
student had 3
samples. Those sam-
ples consisted of:
2. Luciferase is fond in: Fireflies.
3. Luciferase is the negative control.
in our
experiment:
Yes it is expressed.
4. Is PC2 expressed in
pla- narians?
positive control.
5. Since PC2 is
expressed in
planarians, that
would mean that it is
the
in our
experiment.
6. The full name for PC2 is: Prohormone Convertase.
7. Methods for Analyzing chromatin status, analyzing occupancy of promoter by
detecting gene RNA Pol II.
expression using
DNA could include:
1/
93
, Molecular Biology Exam test queries and
answers graded A+
8. Methods for Analyzing RNA levels.
detecting gene
expression using
RNA could include:
Northern blot.
9. What do we use to
mea- sure the RNA
levels?
10. The two types of hybridization based, or PCR-based.
North- ern Blotting
are:
single, transcriptome-wide.
11. In a hybridization-
based Northern blot,
the strands are
and the
limits are - single (RT-qPCR) and the limits are genome-wide (RNAseq).
12. In PCR-based
Northern blotting,
the strands are
and the limits Western blotting, mass spectrometry, ribosome profiling.
are
-
13. The methods for
detect- ing gene
expression are:
2/
93
, Molecular Biology Exam test queries and
answers graded A+
14. Western blotting involves single, antibody.
strands and is
based
3/
93
, Molecular Biology Exam test queries and
answers graded A+
15. Mass-spectrometry is proteome.
wide
16. Ribosome profiling is proteome (predicted).
wide
17. How do we Sanger sequencing.
sequence DNA?
18. In 1953, the helical Watson and Crick.
struc- ture was
published by
19. Where are the nu- cleotides?
attach- ment points
for the in- coming
nucleotides in a RNA
/ DNA strand?
20. What part of the nu-
cleotide attaches to
the 3' hydroxy group?
21. What kind of bond
is formed between
4/
93
answers graded A+
1. In the PCR samples 2 genes of interest and 1 control.
we made, each
student had 3
samples. Those sam-
ples consisted of:
2. Luciferase is fond in: Fireflies.
3. Luciferase is the negative control.
in our
experiment:
Yes it is expressed.
4. Is PC2 expressed in
pla- narians?
positive control.
5. Since PC2 is
expressed in
planarians, that
would mean that it is
the
in our
experiment.
6. The full name for PC2 is: Prohormone Convertase.
7. Methods for Analyzing chromatin status, analyzing occupancy of promoter by
detecting gene RNA Pol II.
expression using
DNA could include:
1/
93
, Molecular Biology Exam test queries and
answers graded A+
8. Methods for Analyzing RNA levels.
detecting gene
expression using
RNA could include:
Northern blot.
9. What do we use to
mea- sure the RNA
levels?
10. The two types of hybridization based, or PCR-based.
North- ern Blotting
are:
single, transcriptome-wide.
11. In a hybridization-
based Northern blot,
the strands are
and the
limits are - single (RT-qPCR) and the limits are genome-wide (RNAseq).
12. In PCR-based
Northern blotting,
the strands are
and the limits Western blotting, mass spectrometry, ribosome profiling.
are
-
13. The methods for
detect- ing gene
expression are:
2/
93
, Molecular Biology Exam test queries and
answers graded A+
14. Western blotting involves single, antibody.
strands and is
based
3/
93
, Molecular Biology Exam test queries and
answers graded A+
15. Mass-spectrometry is proteome.
wide
16. Ribosome profiling is proteome (predicted).
wide
17. How do we Sanger sequencing.
sequence DNA?
18. In 1953, the helical Watson and Crick.
struc- ture was
published by
19. Where are the nu- cleotides?
attach- ment points
for the in- coming
nucleotides in a RNA
/ DNA strand?
20. What part of the nu-
cleotide attaches to
the 3' hydroxy group?
21. What kind of bond
is formed between
4/
93
