Molecular biology Exam test queries
and answers graded A+
What is the location of most hydrophobic residues in a typical water soluble protein in its folded
functional conformation? - ANS✅✅26 (In the interior of the protein)
In bacteria RNA polymerase itself does not recognize promoters. Instead, RNA bacterial polymerase
forms a complex with a protein that helps recognize the promoter. This protein is: - ANS✅✅48
(Sigma factor)
Which task can you perform using a FACS (fluorescence activated cell sorter)? - ANS✅✅30 (Isolate
cells to which an antibody tagged with a fluorescent dye is bound)
The overall rate of transcription is greatly reduced during mitosis because of _________ -
ANS✅✅59 or 22 (The tight packing of the highly condensed DNA; Heterochromatin); partial credit
for 52 (The effect of histones on gene expression) Note: The answer is not 54. The ongoing process
of DNA replication. DNA replication occurs in the S phase (Synthesis), which is separated from the M
phase (Mitosis) by the G2 phase (Gap).
Which of the following types of microscopy would allow you to determine that a particular DNA
binding protein is located in the nucleus? - ANS✅✅27 (Indirect immunofluorescence microscopy)
Note: Electron microscopy has sufficient spatial resolution, but how to tell apart one protein from
another? This can be done by first incubating the sample with a labeled antibody against this nuclear
protein.
Which process occurs during horizontal transfer of genes? - ANS✅✅20 (Genes from one organism
are acquired by another organism through the uptake of DNA)
Antibiotics that block bacterial translation can be harmful to human mitochondria. This observation
can be explained by: - ANS✅✅57 (The process of endosymbiosis) Note: 42: "Reductive evolution
reduced mitochondrial ribosomes to their most simple state" unintendedly turned out to be a
popular. Maybe it sounds plausible but this simply is not the case.
The weak molecular interaction that results in the formation of lipid bilayers is: - ANS✅✅53 (The
hydrophobic effect)
and answers graded A+
What is the location of most hydrophobic residues in a typical water soluble protein in its folded
functional conformation? - ANS✅✅26 (In the interior of the protein)
In bacteria RNA polymerase itself does not recognize promoters. Instead, RNA bacterial polymerase
forms a complex with a protein that helps recognize the promoter. This protein is: - ANS✅✅48
(Sigma factor)
Which task can you perform using a FACS (fluorescence activated cell sorter)? - ANS✅✅30 (Isolate
cells to which an antibody tagged with a fluorescent dye is bound)
The overall rate of transcription is greatly reduced during mitosis because of _________ -
ANS✅✅59 or 22 (The tight packing of the highly condensed DNA; Heterochromatin); partial credit
for 52 (The effect of histones on gene expression) Note: The answer is not 54. The ongoing process
of DNA replication. DNA replication occurs in the S phase (Synthesis), which is separated from the M
phase (Mitosis) by the G2 phase (Gap).
Which of the following types of microscopy would allow you to determine that a particular DNA
binding protein is located in the nucleus? - ANS✅✅27 (Indirect immunofluorescence microscopy)
Note: Electron microscopy has sufficient spatial resolution, but how to tell apart one protein from
another? This can be done by first incubating the sample with a labeled antibody against this nuclear
protein.
Which process occurs during horizontal transfer of genes? - ANS✅✅20 (Genes from one organism
are acquired by another organism through the uptake of DNA)
Antibiotics that block bacterial translation can be harmful to human mitochondria. This observation
can be explained by: - ANS✅✅57 (The process of endosymbiosis) Note: 42: "Reductive evolution
reduced mitochondrial ribosomes to their most simple state" unintendedly turned out to be a
popular. Maybe it sounds plausible but this simply is not the case.
The weak molecular interaction that results in the formation of lipid bilayers is: - ANS✅✅53 (The
hydrophobic effect)
