Molecular biology exam questions
and answers graded A+
Identify the correct complementary DNA sequence for the oligonucleotide 5'-ACTGATTCATA-3'. -
ANS✅✅5'- TATGAATCAGA -3'
Which of the following describes the second step in a DNA polymerase reaction? - ANS✅✅The 3'-
OH attacks the α phosphorus of the incoming dNTP.
You just joined a lab to design a novel protein to use in place of a core histone protein. Which sets of
amino acids would you prefer to use in your engineered histone protein? - ANS✅✅C. R, H, Y, K, F
How are the epigenetic marks of a particular chromatin state preserved during cell division? -
ANS✅✅H2A-H2B dimers and H3-H4 tetramers come off during replication and assemble with
unmarked histones. These new octamers then rebind to the newly replicated DNA to form
nucleosomes.
Which of the following is true of DNA polymerase I? - ANS✅✅It is responsible for RNA primer
removal and replacement with DNA nucleotides.
The contacts between the DNA and the histones of the nucleosome are: - ANS✅✅primarily
between the DNA and the histone peptide backbone.
Proteins that bind to [acetylated lysine // methylated arginine] residues in the histone tail contain: -
ANS✅✅bromodomains. ß acetylated lysines
chromodomains. ß methylated arginines
Choose the correct order of histone core assembly - ANS✅✅One H3/H4 tetramer binds DNA and
then two H2A/B dimers bind
Which of the following DNA polymerases is the enzyme responsible for the majority of DNA
replication in [eukaryotes / bacteria] - ANS✅✅Pol e ß eukaryotes
and answers graded A+
Identify the correct complementary DNA sequence for the oligonucleotide 5'-ACTGATTCATA-3'. -
ANS✅✅5'- TATGAATCAGA -3'
Which of the following describes the second step in a DNA polymerase reaction? - ANS✅✅The 3'-
OH attacks the α phosphorus of the incoming dNTP.
You just joined a lab to design a novel protein to use in place of a core histone protein. Which sets of
amino acids would you prefer to use in your engineered histone protein? - ANS✅✅C. R, H, Y, K, F
How are the epigenetic marks of a particular chromatin state preserved during cell division? -
ANS✅✅H2A-H2B dimers and H3-H4 tetramers come off during replication and assemble with
unmarked histones. These new octamers then rebind to the newly replicated DNA to form
nucleosomes.
Which of the following is true of DNA polymerase I? - ANS✅✅It is responsible for RNA primer
removal and replacement with DNA nucleotides.
The contacts between the DNA and the histones of the nucleosome are: - ANS✅✅primarily
between the DNA and the histone peptide backbone.
Proteins that bind to [acetylated lysine // methylated arginine] residues in the histone tail contain: -
ANS✅✅bromodomains. ß acetylated lysines
chromodomains. ß methylated arginines
Choose the correct order of histone core assembly - ANS✅✅One H3/H4 tetramer binds DNA and
then two H2A/B dimers bind
Which of the following DNA polymerases is the enzyme responsible for the majority of DNA
replication in [eukaryotes / bacteria] - ANS✅✅Pol e ß eukaryotes
