uams micro exam 5 review UPDATED ACTUAL Questions and CORRECT Answers
What disease does M. tuberculosis cause? TB (more likely in HIV & elderly)
What disease does M. bovis cause? TB
What disease does M. leprae cause? leprosy (Hansen's disease)
What disease does M. ulcerans cause? ulcer
, What disease does M. kansasii cause? pulmonary
What disease does M. marinum cause? Swimming pool granuloma
What disease does M. scrofulaceum cause? lymphadenitis
What disease does M. avium complex Pulmonary - disseminates in immunocompromised patients
cause?
What disease does M. fortuitum-chelonae M. fortuitum-chelonae-wound infections; M. abscessus-pulmonary infections
cause? M. abscessus?
Name a nonpathogenic AFB species. M. gordonae
Which organism is considered the tap M. gordonae
water scotochromogen?
How should normally sterile body fluids be Centrifuged & sediment used for stains & media
processed for mycobacteria?
Which body site is normally affected by M. Skin & can disseminate in AIDS patients
haemophilum infections?
What are the recommended collection Collect AM sputum samples; 3-5 over several days; 5-10 mls
procedures for isolating mycobacteria from
sputum?
What are the recommended collection Collect AM specimens; 3-5 over several days; 5-10 mls
procedures for isolating mycobacteria from
urine?
What reagents are used in the most N-acetyl-l-cystaine (NALC) & 2% NaOH
common decontamination procedure?
Which specimens should be Specimens with normal flora
decontaminated for mycobacteria
isolation?
Identify the reagents used in the Kinyoun Carbolfuchsin, acid alcohol, & methylene blue
stain.
Identify the functions of the reagents used CF (staining agent), AA (decolorizer), & MB (counterstain)
in the Kinyoun & Ziehl-Neelson stains:
Carbolfuchsin (CF)Acid alcohol
(AA)Methylene blue (MB)
Identify the reagents used in the Ziehl- Heated carbolifuchsin, acid alcohol, & methylene blue
Neelson stain.
Identify the reagents used in the Auramine-o-rhodamine, acid alcohol, & potassium permanganate
fluorescent stain.
Identify the functions of the reagents used AOR-stains, AA-decolorizer, & PP-quences background fluorescence
in the fluorescent stain: Auramine-o-
rhodamine (AOR)Acid alcohol
(AA)Potassium permanganate (PP)
What is the purpose of using the Typically used to confirm a positive fluorochrome stain
Kinyoun/Ziehl-Neelson stain?
Describe a positive Kinyoun/Ziehl Neelson Red rods against a blue background
stain.
What is the purpose of using the Used by most labs as screening stain
fluorescent stain?
Describe a positive fluorescent stain. Yellow to orange fluorescence against a black background
What disease does M. tuberculosis cause? TB (more likely in HIV & elderly)
What disease does M. bovis cause? TB
What disease does M. leprae cause? leprosy (Hansen's disease)
What disease does M. ulcerans cause? ulcer
, What disease does M. kansasii cause? pulmonary
What disease does M. marinum cause? Swimming pool granuloma
What disease does M. scrofulaceum cause? lymphadenitis
What disease does M. avium complex Pulmonary - disseminates in immunocompromised patients
cause?
What disease does M. fortuitum-chelonae M. fortuitum-chelonae-wound infections; M. abscessus-pulmonary infections
cause? M. abscessus?
Name a nonpathogenic AFB species. M. gordonae
Which organism is considered the tap M. gordonae
water scotochromogen?
How should normally sterile body fluids be Centrifuged & sediment used for stains & media
processed for mycobacteria?
Which body site is normally affected by M. Skin & can disseminate in AIDS patients
haemophilum infections?
What are the recommended collection Collect AM sputum samples; 3-5 over several days; 5-10 mls
procedures for isolating mycobacteria from
sputum?
What are the recommended collection Collect AM specimens; 3-5 over several days; 5-10 mls
procedures for isolating mycobacteria from
urine?
What reagents are used in the most N-acetyl-l-cystaine (NALC) & 2% NaOH
common decontamination procedure?
Which specimens should be Specimens with normal flora
decontaminated for mycobacteria
isolation?
Identify the reagents used in the Kinyoun Carbolfuchsin, acid alcohol, & methylene blue
stain.
Identify the functions of the reagents used CF (staining agent), AA (decolorizer), & MB (counterstain)
in the Kinyoun & Ziehl-Neelson stains:
Carbolfuchsin (CF)Acid alcohol
(AA)Methylene blue (MB)
Identify the reagents used in the Ziehl- Heated carbolifuchsin, acid alcohol, & methylene blue
Neelson stain.
Identify the reagents used in the Auramine-o-rhodamine, acid alcohol, & potassium permanganate
fluorescent stain.
Identify the functions of the reagents used AOR-stains, AA-decolorizer, & PP-quences background fluorescence
in the fluorescent stain: Auramine-o-
rhodamine (AOR)Acid alcohol
(AA)Potassium permanganate (PP)
What is the purpose of using the Typically used to confirm a positive fluorochrome stain
Kinyoun/Ziehl-Neelson stain?
Describe a positive Kinyoun/Ziehl Neelson Red rods against a blue background
stain.
What is the purpose of using the Used by most labs as screening stain
fluorescent stain?
Describe a positive fluorescent stain. Yellow to orange fluorescence against a black background
