BIOL 4003 EXAM 2 QUESTIONS AND ANSWERS
100% CORRECT
Which of the following is not one of the four criteria that the genetic material must fulfill to
function?
a. store information
b. replication
c. DNA repair
d. transmission - ANSWER c
You have analyzed the composition of the genetic material in a virus and found the following:
32% adenine, 31% uracil, 18% guanine and 19% cytosine. From this analysis, which of the
following is the most likely structure of the viral genome?
a. single-stranded DNA
b. double-stranded DNA
c. single-stranded RNA
d. double-stranded RNA - ANSWER d
DNA is able to store information because of
a. the 5' to 3' direction of the strands
b. the sequence of the bases
c. the relative locations of the major and minor grooves
,d. the spiral, double-helical structure - ANSWER b
The following question is about DNA replication. In the drawing below, the top strand is the
template DNA. After the middle RNA primer (located between the two upward pointing
arrows) is removed and the gap is filled with DNA, where does DNA ligase function?
a. on the 5' end of the Okazaki fragment
b. on the 3' end of the Okazaki fragment
c. both arrows
d. neither arrow - ANSWER b
Two circular DNA molecules are topoisomers of each other; they contain the same amount of
DNA. Let's call them DNA-1 and DNA-2. Under the electron microscope, DNA-2 is more
compact compared to DNA-1. The level of gene transcription is lower for DNA-1. Which of the
following possibilities would best explain these observations?
a. DNA-1 has 4 negative supercoils and DNA-2 has 2 positive supercoils
b. DNA-1 has 4 positive supercoils and DNA-2 has 2 negative supercoils
c. DNA-1 has 2 positive supercoils and DNA-2 has 4 negative supercoils
d. DNA-1 has 2 positive supercoils and DNA-2 has 2 positive supercoils - ANSWER c
Which of the following is the correct order for the hierarchy of compaction leading to
metaphase chromosome?
a. formation of nucleosomes, formation of a 30 fiber, formation of loop domains, further
compaction of loop domains
, b. formation of nucleosomes, formation of loop domains, further compaction of loop
domains, formation of a 30 fiber
c. formation of a 30 fiber, formation of nucleosomes, formation of loop domaines, further
compaction of loop domains
d. formation of loop domains, further compaction of loop domains, formation of
nucleosomes, formation of a 30 fiber - ANSWER a
In the lagging strand, DNA is made in the direction _____ the replication fork and is made as
_______.
a. toward, one continuous strand
b. away from, one continuous strand
c. toward, Okazaki fragments
d. away from, Okazaki fragments - ANSWER d
At the E coli origin of replication, the function of DnaA protein is
a. to bind DnaA boxes and separate the AT rich region
b. to coat the DNA to prevent it from re-forming a double helix
c. to promote the binding of DNA polymerase to the origin
d. to promote the methylation of the GATC site - ANSWER a
A gene encodes a pre-mRNA with 8 axons and 7 introns. A mutation eliminates the 3' splice
site from intron 5. What exons would be found in the mRNA after splicing was complete?
100% CORRECT
Which of the following is not one of the four criteria that the genetic material must fulfill to
function?
a. store information
b. replication
c. DNA repair
d. transmission - ANSWER c
You have analyzed the composition of the genetic material in a virus and found the following:
32% adenine, 31% uracil, 18% guanine and 19% cytosine. From this analysis, which of the
following is the most likely structure of the viral genome?
a. single-stranded DNA
b. double-stranded DNA
c. single-stranded RNA
d. double-stranded RNA - ANSWER d
DNA is able to store information because of
a. the 5' to 3' direction of the strands
b. the sequence of the bases
c. the relative locations of the major and minor grooves
,d. the spiral, double-helical structure - ANSWER b
The following question is about DNA replication. In the drawing below, the top strand is the
template DNA. After the middle RNA primer (located between the two upward pointing
arrows) is removed and the gap is filled with DNA, where does DNA ligase function?
a. on the 5' end of the Okazaki fragment
b. on the 3' end of the Okazaki fragment
c. both arrows
d. neither arrow - ANSWER b
Two circular DNA molecules are topoisomers of each other; they contain the same amount of
DNA. Let's call them DNA-1 and DNA-2. Under the electron microscope, DNA-2 is more
compact compared to DNA-1. The level of gene transcription is lower for DNA-1. Which of the
following possibilities would best explain these observations?
a. DNA-1 has 4 negative supercoils and DNA-2 has 2 positive supercoils
b. DNA-1 has 4 positive supercoils and DNA-2 has 2 negative supercoils
c. DNA-1 has 2 positive supercoils and DNA-2 has 4 negative supercoils
d. DNA-1 has 2 positive supercoils and DNA-2 has 2 positive supercoils - ANSWER c
Which of the following is the correct order for the hierarchy of compaction leading to
metaphase chromosome?
a. formation of nucleosomes, formation of a 30 fiber, formation of loop domains, further
compaction of loop domains
, b. formation of nucleosomes, formation of loop domains, further compaction of loop
domains, formation of a 30 fiber
c. formation of a 30 fiber, formation of nucleosomes, formation of loop domaines, further
compaction of loop domains
d. formation of loop domains, further compaction of loop domains, formation of
nucleosomes, formation of a 30 fiber - ANSWER a
In the lagging strand, DNA is made in the direction _____ the replication fork and is made as
_______.
a. toward, one continuous strand
b. away from, one continuous strand
c. toward, Okazaki fragments
d. away from, Okazaki fragments - ANSWER d
At the E coli origin of replication, the function of DnaA protein is
a. to bind DnaA boxes and separate the AT rich region
b. to coat the DNA to prevent it from re-forming a double helix
c. to promote the binding of DNA polymerase to the origin
d. to promote the methylation of the GATC site - ANSWER a
A gene encodes a pre-mRNA with 8 axons and 7 introns. A mutation eliminates the 3' splice
site from intron 5. What exons would be found in the mRNA after splicing was complete?
