Denise M. Harmening
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,22 PART I Fundamental Concepts
7. What is the minimum number of platelets required in a
platelet concentrate prepared from whole blood by cen-
trifugation (90% of sampled units)?
1. What is the maximum volume of blood that can be col-
lected from a 110-lb donor, including samples for a. 5.5 3 1011
processing? b. 3 3 1010
c. 3 3 1011
a. 450 mL
d. 5.5 3 1010
b. 500 mL
c. 525 mL 8. RBCs can be frozen for:
d. 550 mL a. 12 months.
2. How often can a blood donor donate whole blood? b. 1 year.
c. 5 years.
a. Every 24 hours
d. 10 years.
b. Once a month
c. Every 8 weeks 9. What is the minimum number of platelets required in
d. Twice a year an apheresis component (90% of the sampled units)?
3. When RBCs are stored, there is a “shift to the left.” This a. 3 3 1011
means: b. 4 3 1011
c. 2 3 1011
a. Hemoglobin oxygen affinity increases, owing to an
d. 3.5 3 1011
increase in 2,3-DPG.
b. Hemoglobin oxygen affinity increases, owing to a de- 10. Whole blood and RBC units are stored at what
crease in 2,3-DPG. temperature?
c. Hemoglobin oxygen affinity decreases, owing to a de- a. 1°C to 6°C
crease in 2,3-DPG. b. 20°C to 24°C
d. Hemoglobin oxygen affinity decreases, owing to an c. 37°C
increase in 2,3-DPG. d. 24°C to 27°C
4. The majority of platelets transfused in the United States 11. Additive solutions are approved for storage of red blood
today are: cells for how many days?
a. Whole blood–derived platelets prepared by the a. 21
platelet-rich plasma method. b. 42
b. Whole blood–derived platelets prepared by the buffy c. 35
coat method. d. 7
c. Apheresis platelets.
d. Prestorage pooled platelets. 12. One criterion used by the FDA for approval of new preser-
vation solutions and storage containers is an average
5. Which of the following anticoagulant preservatives pro- 24-hour post-transfusion RBC survival of more than:
vides a storage time of 35 days at 1°C to 6°C for units of a. 50%.
whole blood and prepared RBCs if an additive solution b. 60%.
is not added? c. 65%.
a. ACD-A d. 75%.
b. CP2D
c. CPD 13. What is the lowest allowable pH for a platelet compo-
d. CPDA-1 nent at outdate?
a. 6
6. What are the current storage time and storage tempera- b. 5.9
ture for platelet concentrates and apheresis platelet c. 6.8
components? d. 6.2
a. 5 days at 1°C to 6°C
b. 5 days at 24°C to 27°C 14. Frozen and thawed RBCs processed in an open system
c. 5 days at 20°C to 24°C can be stored for how many days/hours?
d. 7 days at 22°C to 24°C a. 3 days
b. 6 hours
c. 24 hours
d. 15 days
, Chapter 1 Red Blood Cell and Platelet Preservation: Historical Perspectives and Current Trends 23
15. What is the hemoglobin source for hemoglobin-based
oxygen carriers in advanced clinical testing?
a. Only bovine hemoglobin
b. Only human hemoglobin
c. Both bovine and human hemoglobins
d. None of the above
16. Which of the following occurs during storage of red
blood cells?
a. pH decreases
b. 2,3-DPG increases
c. ATP increases
d. plasma K+ decreases
17. Nucleic acid amplification testing is used to test donor
blood for which of the following infectious diseases?
a. Hepatitis C virus
b. Human immunodeficiency virus
c. West Nile virus
d. All of the above
18. Which of the following is NOT an FDA-approved test
for quality control of platelets?
a. BacT/ALERT
b. eBDS
c. Gram stain
d. Pan Genera Detection (PGD) test
19. Prestorage pooled platelets can be stored for:
a. 4 hours.
b. 24 hours.
c. 5 days.
d. 7 days.
20. Which of the following is the most common cause of
bacterial contamination of platelet products?
a. Entry of skin plugs into the collection bag
b. Environmental contamination during processing
c. Bacteremia in the donor
d. Incorrect storage temperature
, Chapter 2 Basic Genetics 43
9. Transcription can be defined as:
a. Introduction of DNA into cultured cells.
1. Which of the following statements best describes mitosis? b. Reading of mRNA by the ribosome.
c. Synthesis of RNA using DNA as a template.
a. Genetic material is quadruplicated, equally divided
d. Removal of external sequences to form a mature RNA
between four daughter cells
molecule.
b. Genetic material is duplicated, equally divided
between two daughter cells 10. When a male possesses a phenotypic trait that he passes
c. Genetic material is triplicated, equally divided to all his daughters and none of his sons, the trait is said
between three daughter cells to be:
d. Genetic material is halved, doubled, then equally a. X-linked dominant.
divided between two daughter cells b. X-linked recessive.
2. When a recessive trait is expressed, it means that: c. Autosomal dominant.
d. Autosomal recessive.
a. One gene carrying the trait was present.
b. Two genes carrying the trait were present. 11. When a female possesses a phenotypic trait that she
c. No gene carrying the trait was present. passes to all of her sons and none of her daughters, the
d. The trait is present but difficult to observe. trait is said to be:
3. In a pedigree, the “index case” is another name for: a. X-linked dominant.
b. X-linked recessive.
a. Stillbirth.
c. Autosomal dominant.
b. Consanguineous mating.
d. Autosomal recessive.
c. Propositus.
d. Monozygotic twins. 12. DNA is replicated:
4. Which of the following nitrogenous bases make up DNA? a. Semiconservatively from DNA.
b. In a random manner from RNA.
a. Adenine, leucine, guanine, thymine
c. By copying protein sequences from RNA.
b. Alanine, cytosine, guanine, purine
d. By first copying RNA from protein.
c. Isoleucine, lysine, uracil, leucine
d. Adenine, cytosine, guanine, thymine 13. RNA is processed:
5. Proteins and peptides are composed of: a. After RNA is copied from DNA template.
b. After protein folding and unfolding on the ribosome.
a. Golgi bodies grouped together.
c. Before DNA is copied from DNA template.
b. Paired nitrogenous bases.
d. After RNA is copied from protein on ribosomes.
c. Nuclear basic particles.
d. Linear arrangements of amino acids. 14. Translation of proteins from RNA takes place:
6. Which phenotype(s) could not result from the mating a. On the ribosomes in the cytoplasm of the cell.
of a Jk(a+b+) female and a Jk(a-b+) male? b. On the nuclear membrane.
c. Usually while attached to nuclear pores.
a. Jk(a+b–)
d. Inside the nucleolus of the cell.
b. Jk(a+b+)
c. Jk(a–b+) 15. Meiosis is necessary to:
d. Jk(a–b-) a. Keep the N number of the cell consistent within
7. Exon refers to: populations.
b. Prepare RNA for transcription.
a. The part of a gene that contains nonsense mutations.
c. Generate new DNA sequences in daughter cells.
b. The coding region of a gene.
d. Stabilize proteins being translated on the ribosome.
c. The noncoding region of a gene.
d. The enzymes used to cut DNA into fragments.
8. PCR technology can be used to:
a. Amplify small amounts of DNA.
b. Isolate intact nuclear RNA.
c. Digest genomic DNA into small fragments.
d. Repair broken pieces of DNA.
odern
1Wransfusi
Practiu
€
6TH EDITION
DavisP
DavisPlus.fada
,22 PART I Fundamental Concepts
7. What is the minimum number of platelets required in a
platelet concentrate prepared from whole blood by cen-
trifugation (90% of sampled units)?
1. What is the maximum volume of blood that can be col-
lected from a 110-lb donor, including samples for a. 5.5 3 1011
processing? b. 3 3 1010
c. 3 3 1011
a. 450 mL
d. 5.5 3 1010
b. 500 mL
c. 525 mL 8. RBCs can be frozen for:
d. 550 mL a. 12 months.
2. How often can a blood donor donate whole blood? b. 1 year.
c. 5 years.
a. Every 24 hours
d. 10 years.
b. Once a month
c. Every 8 weeks 9. What is the minimum number of platelets required in
d. Twice a year an apheresis component (90% of the sampled units)?
3. When RBCs are stored, there is a “shift to the left.” This a. 3 3 1011
means: b. 4 3 1011
c. 2 3 1011
a. Hemoglobin oxygen affinity increases, owing to an
d. 3.5 3 1011
increase in 2,3-DPG.
b. Hemoglobin oxygen affinity increases, owing to a de- 10. Whole blood and RBC units are stored at what
crease in 2,3-DPG. temperature?
c. Hemoglobin oxygen affinity decreases, owing to a de- a. 1°C to 6°C
crease in 2,3-DPG. b. 20°C to 24°C
d. Hemoglobin oxygen affinity decreases, owing to an c. 37°C
increase in 2,3-DPG. d. 24°C to 27°C
4. The majority of platelets transfused in the United States 11. Additive solutions are approved for storage of red blood
today are: cells for how many days?
a. Whole blood–derived platelets prepared by the a. 21
platelet-rich plasma method. b. 42
b. Whole blood–derived platelets prepared by the buffy c. 35
coat method. d. 7
c. Apheresis platelets.
d. Prestorage pooled platelets. 12. One criterion used by the FDA for approval of new preser-
vation solutions and storage containers is an average
5. Which of the following anticoagulant preservatives pro- 24-hour post-transfusion RBC survival of more than:
vides a storage time of 35 days at 1°C to 6°C for units of a. 50%.
whole blood and prepared RBCs if an additive solution b. 60%.
is not added? c. 65%.
a. ACD-A d. 75%.
b. CP2D
c. CPD 13. What is the lowest allowable pH for a platelet compo-
d. CPDA-1 nent at outdate?
a. 6
6. What are the current storage time and storage tempera- b. 5.9
ture for platelet concentrates and apheresis platelet c. 6.8
components? d. 6.2
a. 5 days at 1°C to 6°C
b. 5 days at 24°C to 27°C 14. Frozen and thawed RBCs processed in an open system
c. 5 days at 20°C to 24°C can be stored for how many days/hours?
d. 7 days at 22°C to 24°C a. 3 days
b. 6 hours
c. 24 hours
d. 15 days
, Chapter 1 Red Blood Cell and Platelet Preservation: Historical Perspectives and Current Trends 23
15. What is the hemoglobin source for hemoglobin-based
oxygen carriers in advanced clinical testing?
a. Only bovine hemoglobin
b. Only human hemoglobin
c. Both bovine and human hemoglobins
d. None of the above
16. Which of the following occurs during storage of red
blood cells?
a. pH decreases
b. 2,3-DPG increases
c. ATP increases
d. plasma K+ decreases
17. Nucleic acid amplification testing is used to test donor
blood for which of the following infectious diseases?
a. Hepatitis C virus
b. Human immunodeficiency virus
c. West Nile virus
d. All of the above
18. Which of the following is NOT an FDA-approved test
for quality control of platelets?
a. BacT/ALERT
b. eBDS
c. Gram stain
d. Pan Genera Detection (PGD) test
19. Prestorage pooled platelets can be stored for:
a. 4 hours.
b. 24 hours.
c. 5 days.
d. 7 days.
20. Which of the following is the most common cause of
bacterial contamination of platelet products?
a. Entry of skin plugs into the collection bag
b. Environmental contamination during processing
c. Bacteremia in the donor
d. Incorrect storage temperature
, Chapter 2 Basic Genetics 43
9. Transcription can be defined as:
a. Introduction of DNA into cultured cells.
1. Which of the following statements best describes mitosis? b. Reading of mRNA by the ribosome.
c. Synthesis of RNA using DNA as a template.
a. Genetic material is quadruplicated, equally divided
d. Removal of external sequences to form a mature RNA
between four daughter cells
molecule.
b. Genetic material is duplicated, equally divided
between two daughter cells 10. When a male possesses a phenotypic trait that he passes
c. Genetic material is triplicated, equally divided to all his daughters and none of his sons, the trait is said
between three daughter cells to be:
d. Genetic material is halved, doubled, then equally a. X-linked dominant.
divided between two daughter cells b. X-linked recessive.
2. When a recessive trait is expressed, it means that: c. Autosomal dominant.
d. Autosomal recessive.
a. One gene carrying the trait was present.
b. Two genes carrying the trait were present. 11. When a female possesses a phenotypic trait that she
c. No gene carrying the trait was present. passes to all of her sons and none of her daughters, the
d. The trait is present but difficult to observe. trait is said to be:
3. In a pedigree, the “index case” is another name for: a. X-linked dominant.
b. X-linked recessive.
a. Stillbirth.
c. Autosomal dominant.
b. Consanguineous mating.
d. Autosomal recessive.
c. Propositus.
d. Monozygotic twins. 12. DNA is replicated:
4. Which of the following nitrogenous bases make up DNA? a. Semiconservatively from DNA.
b. In a random manner from RNA.
a. Adenine, leucine, guanine, thymine
c. By copying protein sequences from RNA.
b. Alanine, cytosine, guanine, purine
d. By first copying RNA from protein.
c. Isoleucine, lysine, uracil, leucine
d. Adenine, cytosine, guanine, thymine 13. RNA is processed:
5. Proteins and peptides are composed of: a. After RNA is copied from DNA template.
b. After protein folding and unfolding on the ribosome.
a. Golgi bodies grouped together.
c. Before DNA is copied from DNA template.
b. Paired nitrogenous bases.
d. After RNA is copied from protein on ribosomes.
c. Nuclear basic particles.
d. Linear arrangements of amino acids. 14. Translation of proteins from RNA takes place:
6. Which phenotype(s) could not result from the mating a. On the ribosomes in the cytoplasm of the cell.
of a Jk(a+b+) female and a Jk(a-b+) male? b. On the nuclear membrane.
c. Usually while attached to nuclear pores.
a. Jk(a+b–)
d. Inside the nucleolus of the cell.
b. Jk(a+b+)
c. Jk(a–b+) 15. Meiosis is necessary to:
d. Jk(a–b-) a. Keep the N number of the cell consistent within
7. Exon refers to: populations.
b. Prepare RNA for transcription.
a. The part of a gene that contains nonsense mutations.
c. Generate new DNA sequences in daughter cells.
b. The coding region of a gene.
d. Stabilize proteins being translated on the ribosome.
c. The noncoding region of a gene.
d. The enzymes used to cut DNA into fragments.
8. PCR technology can be used to:
a. Amplify small amounts of DNA.
b. Isolate intact nuclear RNA.
c. Digest genomic DNA into small fragments.
d. Repair broken pieces of DNA.
