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BIO 251 EXAM -2 |QUESTIONS AND 100% CORRECT WELL DETAILED ANSWERS|LATEST UPDATE!!!2025/2026|GUARANTEED PASS|GRADED A+

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BIO 251 EXAM -2 |QUESTIONS AND 100% CORRECT WELL DETAILED ANSWERS|LATEST UPDATE!!!2025/2026|GUARANTEED PASS|GRADED A+

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CORRECT WELL DETAILED ANSWERS|LATEST
UPDATE!!!2025/2026|GUARANTEED

the study of how the heredity material (DNA and RNA) is transferred. -
ANSWER genetics



all genetic material in a cell - ANSWER genome



what is the sub division of genome - ANSWER - chromosome

- plasmid



what is the sub division of chromosomes - ANSWER genes (coding region)

non-coding region



what composes the DNA back bone - ANSWER sugar

phosphate



which are the 4 nucleotides in DNA - ANSWER Adenine

Thymine

Cytosine

Guanine



Uracil (only in RNA)




1

,what is the difference between bacterial and Eukaryota chromosomes - ANSWER Most
bacteria have only one circular chromosome, whereas eukaryotes tend to have multiple
linear chromosomes



Why is DNA semi-conservative? - ANSWER When DNA divides, each copy receives one
half of the original DNA and one half new DNA.



enzyme(s) that uncoiled the DNA - ANSWER topoisomerase and gyrase, and begins at
a region called the origin of replication



enzyme that unwinds and begins separating the DNA strands in both directions -
ANSWER helicase



enzyme that stabilize the single stranded DNA - ANSWER single strand binding
proteins



what is required for the addition of nucleotides in DNA replication? - ANSWER a free 3'
hydroxyl group



enzyme that adds an RNA primer to the strand being replicated - ANSWER primase



enzyme that makes sure the proper nucleotide is bound, and connects it to the growing DNA
strand - ANSWER DNA polymerase



DNA lagging strand - ANSWER - DNA is synthesized in pieces

- multiple RNA primers are added

- assembled together by the enzymes DNA -polymerase and ligase



3 main process of DNA replication - ANSWER - initiation



2

,- elongation

- termination



Central Dogma - ANSWER DNA -> RNA -> Protein



The process by which DNA is used to encode proteins



what some of the characteristics of transcription/RNA - ANSWER - Only specific
regions are copies

- RNA strands are produced, which do not bind with the DNA (exist as single strands)

- No proofreading



what are the 5 steps in transcription - ANSWER Step 1: RNA polymerase binds to the
promoter (a regulatory region directly upstream of the gene) and initiates DNA unraveling



Step 2 : RNA polymerase facilitates the assembly of free ribonucleotides into chains of RNA,
using the DNA as a template



Step 3: The RNA polymerase continues along the gene adding to the RNA chain, which does
not permanently pair with the DNA, but exists as a single strand



Step 4: RNA synthesis continues until the RNA polymerase encounters a specific region of
DNA known as a terminator



Step 5: Once the terminator is reached, the RNA polymerase stalls and a rho termination
protein facilitates the RNA polymerase and RNA strand to disassociate from the DNA

The DNA is re-wound and the RNA is free to go on to the next step



name for groups of 3 base pairs of the mRNA - ANSWER codons


3

, how many codons are non-coding or stop codons? - ANSWER 3 are non-coding or stop
codons

only 61 of the 64 encode amino acids



which molecule can bind both RNA and amino acids - ANSWER T-RNA



group of three bases on a tRNA molecule that are complementary to an mRNA codon -
ANSWER Anticodon



t-RNA is considered to be "charged" - ANSWER when an amino acid is bound to t-RNA



how many different t-RNA there are - ANSWER 64, one for each codon



the structures where protein synthesis (translation) occurs - ANSWER Ribosomes



which are the 2 microbial ribosome subunits - ANSWER the 30S and 50S, which come
together to form a functional 70S ribosome



what does the "S" in ribosomal subunits stands for - ANSWER Svedberg units, which
measures the rate of sedimentation during centrifugation



what are the 3 functional domains of the large microbial ribosome subunit -
ANSWER Aminoacyl site (A)

site of entry for tRNA's



Peptidyl (P) site

Site of peptide bond formation



4

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