Bio 340 Quiz Module 6 Test Questions and Answers.

The PCR step during which the double-stranded template molecule separates into two strands is called _____________. - Answer denaturation In agarose gel electrophoresis, DNA will be attracted to the negative electrode. - Answer False Which of the following are true about DNA and gel electrophoresis? - Answer All of the above: DNA is negatively charged, Small bands travel more quickly than large bands, The size of a DNA fragment is measured in base pairs Regarding Sanger's sequencing method, why does the incorporation of a ddNTP in the growing complementary strand terminates the process of DNA replication? - Answer Because the ddNTP cannot form a phosphodiester bond with the sugar from a newly recruited dNTP due to the missing hydroxyl group (-OH). The __________ blot technique is used to find an RNA fragment within a sample that is complementary to a DNA probe. - Answer Northern Insulin produced by molecular cloning: - Answer is a recombinant protein All of the following are processes used to introduce DNA molecules into bacterial cells except: - Answer transcription Which of the following is required for repairing the phosphodiester backbone of DNA during molecular cloning? - Answer DNA ligase In blue-white screening, what do blue colonies represent? - Answer Cells containing empty plasmid vectors. In the reproductive cloning of an animal, the genome of the cloned individual comes from ________. - Answer a body cell How are genomic libraries created? - Answer There are multiple methods - the main one uses molecular cloning to amplify fragments of genomes for further study and addition to a collection (the genomic library).