CHAMBERLAIN MICROBIOLOGY Exam 1 2025
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Conjugation differs from reproduction because conjugation
A) Replicates DNA.
B) Transfers DNA vertically, to new cells.
C) Transfers DNA horizontally, to cells in the same generation.
D) Transcribes DNA to RNA.
E) Copies RNA to make DNA. - Correct Answer :C) Transfers DNA horizontally, to
cells in the same generation.
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the antibiotic chloramphenicol binds the 50S ribosome. From this information
you can conclude that chloramphenicol
A) Prevents transcription in eukaryotes.
B) Prevents translation in eukaryotes.
C) Prevents transcription in prokaryotes.
D) Prevents translation in prokaryotes.
E) Prevents mRNA-ribosome binding. - Correct Answer :D) Prevents translation
in prokaryotes.
The use of an organism's biochemical processes to create a product is
A. genetic engineering
B. biotechnology
C. recombinant DNA
D. gel electrophoresis
E. gene probes - Correct Answer :biotechnology
Protoplast Fusion - Correct Answer :-Type of way to insert foreign DNA into cells
- Walls of cells can be enzymatically removed forming this and exchange DNA
- 2 cells fuse and exchange DNA
The various techniques by which scientists manipulate DNA in the lab are called
A. genetic engineering
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B. biotechnology
C. recombinant DNA
D. gel electrophoresis
E. gene probes - Correct Answer :genetic engineering
The deliberate removal of genetic material from one organism and combining it
with the genetic material of another organism is a specific technique called
A. genetic engineering
B. biotechnology
C. recombinant DNA technology
D. gel electrophoresis
E. gene probes - Correct Answer :recombinant DNA
Electroporation - Correct Answer :-Type of way to insert foreign DNA into cells
-Transfer of DNA can be enhanced by using an electric field to form minute
pores in cells and then inject the DNA inside
Labeled, known, short stretches of DNA used to detect a specific sequence of
nucleotides in a mixture are
A. genetic engineering
B. biotechnology
C. recombinant DNA
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D. gel electrophoresis
E. gene probes - Correct Answer :gene probes
Microinjection - Correct Answer :-Type of way to insert foreign DNA into cells
- DNA can be introduced into a cell through a glass micropipette
Hfr (High frequency of recombination) cells - Correct Answer :Result when an F
plasmid integrates into prokaryotic chromosome. Hfr cells form conjugation pili
and transfer cellular genes more frequently than normal F+ cells
Electroporation
Protoplast fusion
Injection - gene gun and microinjection - Correct Answer :Artificial
transformation methods
the polymerase chain reaction (PCR) has been used in all of the following fields,
except
A. forensics.
B. evolutionary studies.
C. gene mapping.
D. medicine.
E. None of the choices; it is useful for all these fields. - Correct Answer :E. None
of the choices; it is useful for all these fields.
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