MLT (ASCP) HEMATOLOGY EXAM:
MEDICAL LABORATORY TECHNICIAN
CERTIFIED BY THE AMERICAN SOCIETY
FOR CLINICAL PATHOLOGY (ASCP),
2025/2026 WITH CORRECT/ACCURATE
ANSWERS
It assesses your knowledge of blood cell morphology, common
hematological disorders, and the procedures and principles of laboratory
testing related to blood
AMERICAN SOCIETY FOR CLINICAL
PATHOLOGY (ASCP)
The light-colored zone adjacent to the nucleus in a plasmacyte is the:
A. ribosome
B. chromatin
C. mitochondria
D. Golgi area - CORRECT-ANSWERS--D. Golgi area >>> The light-colored
zone adjacent to the nucleus in a plasmacyte, also known as the
perinuclear halo or "hof," is an area of the cytoplasm that does not stain
well.
This region is a key morphological feature of a mature plasma cell.
The unstained area corresponds to the location of the Golgi apparatus,
which is responsible for packaging and secreting antibodies produced by
the cell.
,The majority of the iron in an adult is found as a constituent of:
A. hemoglobin
B. hemosiderin
C. myoglobin
D. transferrin - CORRECT-ANSWERS--A. hemoglobin >>> Hemoglobin, the
protein found in red blood cells, accounts for approximately 70% of the
body's total iron content. Its primary function is to transport oxygen from
the lungs to tissues throughout the body.
While the other options contain iron, they do so in much smaller
quantities compared to hemoglobin.
The main function of the hexose monophosphate shunt in the erythrocyte
is to:
A. reglate the level of 2,3-DPG
B. provide reduced glutathione to prevent oxidation of hemoglobin
C. prevent the reduction of heme iron
D. provide energy for membrane maintenance - CORRECT-ANSWERS--B.
provide the level 2,3-DPG >>> The hexose monophosphate shunt (also
known as the pentose phosphate pathway) is crucial for red blood cells
because they are constantly exposed to oxygen, which can cause
oxidative damage.
The pathway's main function is to produce NADPH, a molecule with strong
reducing power.
NADPH is then used by the enzyme glutathione reductase to maintain
high levels of reduced glutathione (GSH).
Reduced glutathione is a potent antioxidant that detoxifies hydrogen
peroxide and other free radicals that would otherwise oxidize hemoglobin
and damage the red blood cell membrane.
, Deficiencies in the key enzyme of this pathway, G-6-PD, lead to a buildup
of oxidants and result in a type of hemolytic anemia.
In order for hemoglobin to combine reversibly with oxygen, the iron must
be:
A. complexed with haptoglobin
B. freely circulating in the cytoplasm
C. attached to transferrin
D. in the ferrous state - CORRECT-ANSWERS--D. in the ferrous state >>>
For hemoglobin to effectively bind oxygen for transport throughout the
body, the iron atom within its heme group must be in the ferrous (Fe²⁺)
state.
Oxygen binding to ferrous iron is a reversible process, meaning the
oxygen molecule can be picked up and released as needed.
If the iron is oxidized to the ferric (Fe³⁺) state, the hemoglobin is
converted into methemoglobin (MetHb), which cannot bind to oxygen and
is therefore valueless as a respiratory pigment.
In which of the following disease states are teardrop cells and adnormal
platelets most characteristically seen?
A. hemolytic anemia
B. multiple myeloma
C. G-6-PD deficiency
D. myeloid metaplasma - CORRECT-ANSWERS--D. myeloid metaplasia
In the normal adult, the spleen acts as a site for:
A. storage of red blood cells
B. production of red blood cells
MEDICAL LABORATORY TECHNICIAN
CERTIFIED BY THE AMERICAN SOCIETY
FOR CLINICAL PATHOLOGY (ASCP),
2025/2026 WITH CORRECT/ACCURATE
ANSWERS
It assesses your knowledge of blood cell morphology, common
hematological disorders, and the procedures and principles of laboratory
testing related to blood
AMERICAN SOCIETY FOR CLINICAL
PATHOLOGY (ASCP)
The light-colored zone adjacent to the nucleus in a plasmacyte is the:
A. ribosome
B. chromatin
C. mitochondria
D. Golgi area - CORRECT-ANSWERS--D. Golgi area >>> The light-colored
zone adjacent to the nucleus in a plasmacyte, also known as the
perinuclear halo or "hof," is an area of the cytoplasm that does not stain
well.
This region is a key morphological feature of a mature plasma cell.
The unstained area corresponds to the location of the Golgi apparatus,
which is responsible for packaging and secreting antibodies produced by
the cell.
,The majority of the iron in an adult is found as a constituent of:
A. hemoglobin
B. hemosiderin
C. myoglobin
D. transferrin - CORRECT-ANSWERS--A. hemoglobin >>> Hemoglobin, the
protein found in red blood cells, accounts for approximately 70% of the
body's total iron content. Its primary function is to transport oxygen from
the lungs to tissues throughout the body.
While the other options contain iron, they do so in much smaller
quantities compared to hemoglobin.
The main function of the hexose monophosphate shunt in the erythrocyte
is to:
A. reglate the level of 2,3-DPG
B. provide reduced glutathione to prevent oxidation of hemoglobin
C. prevent the reduction of heme iron
D. provide energy for membrane maintenance - CORRECT-ANSWERS--B.
provide the level 2,3-DPG >>> The hexose monophosphate shunt (also
known as the pentose phosphate pathway) is crucial for red blood cells
because they are constantly exposed to oxygen, which can cause
oxidative damage.
The pathway's main function is to produce NADPH, a molecule with strong
reducing power.
NADPH is then used by the enzyme glutathione reductase to maintain
high levels of reduced glutathione (GSH).
Reduced glutathione is a potent antioxidant that detoxifies hydrogen
peroxide and other free radicals that would otherwise oxidize hemoglobin
and damage the red blood cell membrane.
, Deficiencies in the key enzyme of this pathway, G-6-PD, lead to a buildup
of oxidants and result in a type of hemolytic anemia.
In order for hemoglobin to combine reversibly with oxygen, the iron must
be:
A. complexed with haptoglobin
B. freely circulating in the cytoplasm
C. attached to transferrin
D. in the ferrous state - CORRECT-ANSWERS--D. in the ferrous state >>>
For hemoglobin to effectively bind oxygen for transport throughout the
body, the iron atom within its heme group must be in the ferrous (Fe²⁺)
state.
Oxygen binding to ferrous iron is a reversible process, meaning the
oxygen molecule can be picked up and released as needed.
If the iron is oxidized to the ferric (Fe³⁺) state, the hemoglobin is
converted into methemoglobin (MetHb), which cannot bind to oxygen and
is therefore valueless as a respiratory pigment.
In which of the following disease states are teardrop cells and adnormal
platelets most characteristically seen?
A. hemolytic anemia
B. multiple myeloma
C. G-6-PD deficiency
D. myeloid metaplasma - CORRECT-ANSWERS--D. myeloid metaplasia
In the normal adult, the spleen acts as a site for:
A. storage of red blood cells
B. production of red blood cells
