AAMC FL 4 WITH CORRECT QUESTIONS
AND ANSWERS 2025/2026
A carbonyl group contains what type of bonding interaction between C and O atoms?
A. One σ only
B. One σ and one π only
C. One π only
D. One σ and two π only - correct answers B.
A carbonyl group contains a C=O double bond. The first bonding interaction between atoms is
always a σ. The second bond is formed from π symmetry orbitals.
A defining characteristic of proteins that act as transcription factors (such as STAT5b) is that
they:
A. can dimerize
B. can phosphorylate other proteins
C. contain a DNA binding domain
D. are present within the nucleus of the cell - correct answers C.
The defining characteristic of a transcription factor is that it has a DNA binding domain that
allows it to bind to a regulatory nucleic acid sequence in a gene to alter transcription.
,A peptide consisting of nine amino acids was partially hydrolysed. Three different tripeptides
were isolated. None of the tripeptides share a common amino acid. Based on the data, what is
the total number of possible structures possible for the full-length peptide?
A. 3
B. 4
C. 6
D. 27 - correct answers C.
Each of the tripeptides can appear in the first position and can combine in two possible ways
with the other two tripeptides: 3 x 2=6
A researcher measure the concentration of CO2 (aq) in a solution at equilibrium. Which
additional quantity must be measured in order to calculate the Henry's Law constant kH for the
gas?
A. atmospheric pressure
B. volume of the solvent
C. partial pressure of the gas
D. vapor pressure of water - correct answers C.
The Henry's Law constant kH relates the solubility of a gas S to the pressure of the gas Pg above
the solution and is written as S=kH.Pg. Therefore, in addition to the concentration of CO2 (aq) in
a solution at equilibrium, the partial pressure of the gas Pg must be measured.
A woman with cystic fibrosis considers having children with an asymptomatic man who is
heterozygous for the cystic fibrosis allele. What is probability of having a child with cystic
fibrosis?
,A. 25%
B. 50%
C. 75%
D. 100% - correct answers B.
A heterozygous genotype that results in an asymptomatic phenotype implies that the disorder is
due to a recessive allele. Therefore, a homozygous individual and a heterozygous individual
have 50% probability of having a child with the disorder.
According to Table 1, what is the concentration of the glucose in the blood from which the
diluted sample was taken?
A. 60 mg/dL
B. 90 mg/dL
C. 120 mg/dL
D. 150 mg/dL - correct answers D.
From Table 1, the glucose concentration in the diluted sample is (o.20/0.24) x 6.0 mg/dL = 5.0
mg/dL.
The blood then has a glucose concentration of 30 x 5.0 mg/dL= 150 mg/dL.
According to the IUPAC, which is the systematic name for the hydrocarbon shown?
A. Z-3-methylpent-2-ene
B. E-3-methylpent-2-ene
C. Z-3-ethylbut-2-ene
D. E-3-ethylbut-2-ene - correct answers A.
, By IUPAC rules, first identify the longest unbroken chain of carbon atoms. Next, number the
carbon atoms in this chain starting from the end that gives the C=C the lowest numbers. The
double bond is identified by the portion of the carbon atoms from the lowest numbers ed (2),
and then the methyl group is assigned at the 3-position. The stereochemical designator for the
double bond is X because the highest priority groups occur on the same side of the double
bond. (On Z same side)
An unknown amount of a radioactive isotope with a half-life of 2.0 h was observed for 6.0 h. If
the amount of the isotope remaining after 6.0 h was 24 g, what would the original amount have
been?
A. 3 g
B. 4 g
C. 144 g
D. 192 g - correct answers D.
Given the half-life T=2h, the duration t=6 h represents 3 half lives because 2 h x3 =6 h.
According to the radioactive decay law N(t)=No(1/2) t/T the amount of remaining isotope is
(1/2)^3= 1/8 of the original amount. the original amount was then 8 x 24= 192 g.
As blood passes through actively contracting skeletal muscle tissue, the affinity of hemoglobin
for oxygen in the muscle tissue:
A. increases as a result of an increase in plasma temperature
B. increases as a result of an increase in plasma PO2
C. decreases as a result of a decrease in plasma pH
D. decreases as a result of a decrease in plasma PCO2 - correct answers C.
AND ANSWERS 2025/2026
A carbonyl group contains what type of bonding interaction between C and O atoms?
A. One σ only
B. One σ and one π only
C. One π only
D. One σ and two π only - correct answers B.
A carbonyl group contains a C=O double bond. The first bonding interaction between atoms is
always a σ. The second bond is formed from π symmetry orbitals.
A defining characteristic of proteins that act as transcription factors (such as STAT5b) is that
they:
A. can dimerize
B. can phosphorylate other proteins
C. contain a DNA binding domain
D. are present within the nucleus of the cell - correct answers C.
The defining characteristic of a transcription factor is that it has a DNA binding domain that
allows it to bind to a regulatory nucleic acid sequence in a gene to alter transcription.
,A peptide consisting of nine amino acids was partially hydrolysed. Three different tripeptides
were isolated. None of the tripeptides share a common amino acid. Based on the data, what is
the total number of possible structures possible for the full-length peptide?
A. 3
B. 4
C. 6
D. 27 - correct answers C.
Each of the tripeptides can appear in the first position and can combine in two possible ways
with the other two tripeptides: 3 x 2=6
A researcher measure the concentration of CO2 (aq) in a solution at equilibrium. Which
additional quantity must be measured in order to calculate the Henry's Law constant kH for the
gas?
A. atmospheric pressure
B. volume of the solvent
C. partial pressure of the gas
D. vapor pressure of water - correct answers C.
The Henry's Law constant kH relates the solubility of a gas S to the pressure of the gas Pg above
the solution and is written as S=kH.Pg. Therefore, in addition to the concentration of CO2 (aq) in
a solution at equilibrium, the partial pressure of the gas Pg must be measured.
A woman with cystic fibrosis considers having children with an asymptomatic man who is
heterozygous for the cystic fibrosis allele. What is probability of having a child with cystic
fibrosis?
,A. 25%
B. 50%
C. 75%
D. 100% - correct answers B.
A heterozygous genotype that results in an asymptomatic phenotype implies that the disorder is
due to a recessive allele. Therefore, a homozygous individual and a heterozygous individual
have 50% probability of having a child with the disorder.
According to Table 1, what is the concentration of the glucose in the blood from which the
diluted sample was taken?
A. 60 mg/dL
B. 90 mg/dL
C. 120 mg/dL
D. 150 mg/dL - correct answers D.
From Table 1, the glucose concentration in the diluted sample is (o.20/0.24) x 6.0 mg/dL = 5.0
mg/dL.
The blood then has a glucose concentration of 30 x 5.0 mg/dL= 150 mg/dL.
According to the IUPAC, which is the systematic name for the hydrocarbon shown?
A. Z-3-methylpent-2-ene
B. E-3-methylpent-2-ene
C. Z-3-ethylbut-2-ene
D. E-3-ethylbut-2-ene - correct answers A.
, By IUPAC rules, first identify the longest unbroken chain of carbon atoms. Next, number the
carbon atoms in this chain starting from the end that gives the C=C the lowest numbers. The
double bond is identified by the portion of the carbon atoms from the lowest numbers ed (2),
and then the methyl group is assigned at the 3-position. The stereochemical designator for the
double bond is X because the highest priority groups occur on the same side of the double
bond. (On Z same side)
An unknown amount of a radioactive isotope with a half-life of 2.0 h was observed for 6.0 h. If
the amount of the isotope remaining after 6.0 h was 24 g, what would the original amount have
been?
A. 3 g
B. 4 g
C. 144 g
D. 192 g - correct answers D.
Given the half-life T=2h, the duration t=6 h represents 3 half lives because 2 h x3 =6 h.
According to the radioactive decay law N(t)=No(1/2) t/T the amount of remaining isotope is
(1/2)^3= 1/8 of the original amount. the original amount was then 8 x 24= 192 g.
As blood passes through actively contracting skeletal muscle tissue, the affinity of hemoglobin
for oxygen in the muscle tissue:
A. increases as a result of an increase in plasma temperature
B. increases as a result of an increase in plasma PO2
C. decreases as a result of a decrease in plasma pH
D. decreases as a result of a decrease in plasma PCO2 - correct answers C.
